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Invitrogen
CD166 Recombinant Rabbit Monoclonal Antibody (001)
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CD166 Antibody (MA5-29859) in WB
Western Blot analysis of CD166 (ALCAM) using CD166 Recombinant Monoclonal (Product # MA5-29859) at a 1:500 dilution. Sample: Lysates/proteins at 30 μg per lane. Lane A: SH-SY5Y whole cell lysate; Lane B: Daudi whole cell lysate; Lane C: HuT78 whole cell lysate. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:65 kDa, Observed band size:100 kDa.
Product Details
MA5-29859
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
001
Immunogen
Recombinant Rat CD166/ALCAM protein (Met1-Lys527)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2785676
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
This antibody has specificity for Rat CD166/ALCAM.
Target Information
This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Activated leukocyte cell adhesion molecule; CD166; CD166 antigen; FLJ38514; HB2; KG-CAM; MGC71733; Protein MEMD; SB-10 antigen
Gene Aliases: Alcam
UniProt ID: (Rat) O35112
Entrez Gene ID: (Rat) 79559
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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