Invitrogen

CD171 Monoclonal Antibody (UJ127)

CD171 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 10

CD171 Antibody (MA5-14140) in ICC/IF

Immunofluorescence analysis of CD171 was performed using 70% confluent log phase SW480 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD171 Monoclonal Antibody (UJ127) (Product # MA5-14140) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product ... View More

CD171 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CD171 Antibody in Immunohistochemistry (Frozen) (IHC (F))

CD171 Antibody in Immunoprecipitation (IP)

Product Image Gallery

CD171 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of CD171 was observed in in SW480 and PANC-1 in comparison to Caco-2 using Anti-CD171 Monoclonal Antibody (UJ127) (Product # MA5-14140) in Western Blot. {RE}

Product Details

MA5-14140

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

Immunohistochemistry (Frozen) (IHC (F))

1:1,000

Immunocytochemistry (ICC/IF)

1:200

View 2 publications 2 publications

Flow Cytometry (Flow)

View 2 publications 2 publications

Immunoprecipitation (IP)

2 µg/mg protein lysate

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Pig

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

UJ127

Immunogen

Homogenous suspension of 16 week human fetal brain

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4, with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10979034

Product Specific Information

MA5-14140 targets L1 Cell Adhesion Molecule in immunohistochemistry (paraffin), immunoprecipitation, and Western blot applications and shows reactivity with human samples.

The MA5-14140 immunogen is homogenous suspension of 16 week human fetal brain.

MA5-14140 has been successfully used in immunohistochemistry analysis of NCAM1 in mouse E16.5 frozen lung sections.

Target Information

L1CAM/CD171 is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause three X-linked neurological syndromes known by the acronym CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of a neuron-specific exon is thought to be functionally relevant.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Inhibition of Pannexin 1 Reduces the Tumorigenic Properties of Human Melanoma Cells.

Cancers

Freeman TJ,Sayedyahossein S,Johnston D,Sanchez-Pupo RE,O'Donnell B,Huang K,Lakhani Z,Nouri-Nejad D,Barr KJ,Harland L,Latosinsky S,Grant A,Dagnino L,Penuela S

MA5-14140 was used in Western Blotting to demonstrate that pannexin 1 is highly expressed in human melanoma tumours at all stages of disease progression.

Wed Jan 16 00:00:00 UTC 2019

Targeting FER Kinase Inhibits Melanoma Growth and Metastasis.

Cancers

Ivanova IA,Arulanantham S,Barr K,Cepeda M,Parkins KM,Hamilton AM,Johnston D,Penuela S,Hess DA,Ronald JA,Dagnino L

MA5-14140 was used in Western Blotting to examine the role that Fes-related kinase kinase plays in melanoma at the molecular level.

Sun Mar 24 00:00:00 UTC 2019

Targeting L1 cell adhesion molecule expression using liposome-encapsulated siRNA suppresses prostate cancer bone metastasis and growth.

Oncotarget

Sung SY,Wu IH,Chuang PH,Petros JA,Wu HC,Zeng HJ,Huang WC,Chung LW,Hsieh CL

MA5-14140 was used in immunohistochemistry to determine how using liposome-encapsulated siRNA to target L1 cell adhesion molecule expression suppresses prostate cancer bone metastasis and growth

Thu Oct 30 00:00:00 UTC 2014

Inflammatory responses in the muscle coat of stomach and small bowel in the postoperative ileus model of guinea pig.

Yonsei medical journal

Choi HK,Lee YJ,Lee YH,Park JP,Min K,Park H

MA5-14140 was used in immunohistochemistry to use a guinea pig model of postoperative ileus to study the muscle coat inflammatory responses of the stomach and small intestine

Fri Nov 01 00:00:00 UTC 2013

Adhesion molecule L1 is down-regulated in malignant peripheral nerve sheath tumors versus benign neurofibromatosis type 1-associated tumors.

Oral surgery, oral medicine, oral pathology and oral radiology

Blessmann M,Gröbe A,Quaas A,Kaifi JT,Mistakidis G,Bernreuther C,Sauter G,Gros S,Rawnaq T,Friedrich R,Mautner VF,Smeets R,Heiland M,Schachner M,Izbicki JR

MA5-14140 was used in immunohistochemistry to study the expression of the L1 adhesion molecule in malignant peripheral nerve sheath tumors and benign neurofibromatosis type 1-associated tumors

Wed Feb 01 00:00:00 UTC 2012

Two alcohol binding residues interact across a domain interface of the L1 neural cell adhesion molecule and regulate cell adhesion.

The Journal of biological chemistry

Dou X,Menkari CE,Shanmugasundararaj S,Miller KW,Charness ME

MA5-14140 was used in flow cytometry to characterize two alcohol binding residues in L1 neural cell adhesion molecule and their involvement in cell adhesion

Fri May 06 00:00:00 UTC 2011

DOG1 and CD117 are the antibodies of choice in the diagnosis of gastrointestinal stromal tumours.

Histopathology

Novelli M,Rossi S,Rodriguez-Justo M,Taniere P,Seddon B,Toffolatti L,Sartor C,Hogendoorn PC,Sciot R,Van Glabbeke M,Verweij J,Blay JY,Hohenberger P,Flanagan A,Dei Tos AP

MA5-14140 was used in immunohistochemistry to study the value of COG1 and CD117 immunohistochemistry in the diagnosis of gastrointestinal stromal tumors

Sun Aug 01 00:00:00 UTC 2010

SSEA-1 is an enrichment marker for tumor-initiating cells in human glioblastoma.

Cell stem cell

Son MJ,Woolard K,Nam DH,Lee J,Fine HA

MA5-14140 was used in western blot to investigate the distribution and localization of SSEA-1 in human glioblastoma multiforme cells

Fri May 08 00:00:00 UTC 2009

Subset of esophageal adenocarcinoma expresses adhesion molecule l1 in contrast to squamous cell carcinoma.

Anticancer research

Rawnaq T,Kleinhans H,Uto M,Schurr PG,Reichelt U,Cataldegirmen G,Gawad KA,Yekebas EF,Schachner M,Izbicki JR,Kaifi JT

MA5-14140 was used in immunohistochemistry to study neural cell adhesion molecule L1 overexpression in a subgroup of esophageal cancer

Wed Apr 01 00:00:00 UTC 2009

L1 is associated with micrometastatic spread and poor outcome in colorectal cancer.

Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc

Kaifi JT,Reichelt U,Quaas A,Schurr PG,Wachowiak R,Yekebas EF,Strate T,Schneider C,Pantel K,Schachner M,Sauter G,Izbicki JR

MA5-14140 was used in immunohistochemistry to study the role of the L1 adhesion molecule in micrometastatic spread and poor outcome in colorectal cancer

Thu Nov 01 00:00:00 UTC 2007

L1 is associated with favorable outcome in neuroblastomas in contrast to adult tumors.

Annals of surgical oncology

Wachowiak R,Fiegel HC,Kaifi JT,Quaas A,Krickhahn A,Schurr PG,Erttmann R,Schachner M,Kluth D,Sauter G,Izbicki JR

MA5-14140 was used in immunohistochemistry to study the association of the L1 neuronal adhesion molecule with favorable outcome in childhood neuroblastomas

Sat Dec 01 00:00:00 UTC 2007

L1 (CD171) is highly expressed in gastrointestinal stromal tumors.

Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc

Kaifi JT,Strelow A,Schurr PG,Reichelt U,Yekebas EF,Wachowiak R,Quaas A,Strate T,Schaefer H,Sauter G,Schachner M,Izbicki JR

MA5-14140 was used in immunohistochemistry to study the expression of L1 (CD171) in gastrointestinal stromal tumors

Wed Mar 01 00:00:00 UTC 2006

Identification of cell surface and secreted proteins essential for tumor cell survival using a genetic suppressor element screen.

Oncogene

Gelman MS,Ye XK,Stull R,Suhy D,Jin L,Ng D,Than B,Ji M,Pan A,Perez P,Sun Y,Yeung P,Garcia LM,Harte R,Lu Y,Lamar E,Tavassoli R,Kennedy S,Osborn S,Chin DJ,Meshaw K,Holzmayer TA,Axenovich SA,Abo A

MA5-14140 was used in flow cytometry to identify molecular markers and therapeutic targets for tumors

Thu Oct 21 00:00:00 UTC 2004

Characterization of gene expression in mucinous cystic neoplasms of the pancreas using oligonucleotide microarrays.

Oncogene

Fukushima N,Sato N,Prasad N,Leach SD,Hruban RH,Goggins M

MA5-14140 was used in immunohistochemistry to investigate the changes of gene expression in pancreatic mucinous cystic neoplasms

Thu Dec 02 00:00:00 UTC 2004

Extracellular signal-regulated kinase (ERK)-dependent gene expression contributes to L1 cell adhesion molecule-dependent motility and invasion.

The Journal of biological chemistry

Silletti S,Yebra M,Perez B,Cirulli V,McMahon M,Montgomery AM

MA5-14140 was used in western blot to elucidate the association between L1 expression and motile processes involved in metastasis and development

Fri Jul 09 00:00:00 UTC 2004

Patterning axonal guidance molecules using a novel strategy for microcontact printing.

Neurochemical research

Oliva AA,James CD,Kingman CE,Craighead HG,Banker GA

MA5-14140 was used in western blot to develop a protocol for microprinting proteins on a substrate in order to control neurite growth in culture

Sat Nov 01 00:00:00 UTC 2003

Bioinformatics

Protein Aliases: antigen identified by monoclonal antibody R1; CD 171; CD171; CD171 molecule; N-CAM-L1; Neural cell adhesion molecule L1; sCD171; sL1 CAM; sL1CAM; soluble CD 171; soluble CD171; soluble L1 CAM; soluble L1CAM

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Gene Aliases: CAML1; CD171; HSAS; HSAS1; L1; L1CAM; MASA; MIC5; N-CAM-L1; N-CAML1; NCAM-L1; S10; SPG1

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UniProt ID: (Human) P32004

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Entrez Gene ID: (Human) 3897, (Mouse) 16728

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Function(s)

integrin binding protein binding PDZ domain binding sialic acid binding identical protein binding protein self-association

Process(es)

chemotaxis cell adhesion nervous system development axon guidance positive regulation of axon extension leukocyte migration homophilic cell adhesion via plasma membrane adhesion molecules heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules leukocyte cell-cell adhesion cell surface receptor signaling pathway multicellular organism development positive regulation of cell-cell adhesion cell differentiation neuron projection development cell-cell adhesion mediated by integrin homotypic cell-cell adhesion positive regulation of calcium-mediated signaling

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD171 Monoclonal Antibody (UJ127)

CD171 Antibody (MA5-14140) in ICC/IF

Product Details

MA5-14140

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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