Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
CD172a (SIRP alpha) Monoclonal Antibody (DH59B)
FIGURE: 1 / 1
CD172a (SIRP alpha) Antibody (MA5-28298) in Flow
Flow cytometry of CD172a (SIRP alpha) in bovine peripheral blood monocytes. Sample was incubated with CD172a (SIRP alpha) monoclonal antibody (Product # MA5-28298). Figures are arranged as follows: A) Mouse IgG1 negative control followed by Goat anti Mouse IgG1 - PE. B) Mouse anti Bovine CD172a antibody, clone DH59B followed by Goat anti Mouse IgG1 - PE secondary antibody.
Product Details
MA5-28298
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
DH59B
Immunogen
Cells from multiple species with the final screening of the fusion on cells from dog
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
tissue culture supernatant
Contains
0.09% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2745261
Product Specific Information
This antibody recognizes bovine CD172a also known as SIRP alpha or MyD-1 antigen. CD172a is a transmembrane signal regulatory protein expressed primarily by macrophages, monocytes, dendritic cells, granulocytes, myeloid progenitors, hematopoietic stem cells, and neurons.
Target Information
SIRP alpha (CD172a, signal-regulatory protein alpha) is a receptor-type transmembrane glycoprotein expressed on cells of myeloid origin, including granulocytes, dendritic cells (DCs), macrophages, mast cells and hematopoietic stem cells. SIRP alpha acts as a substrate for several activated tyrosine kinases, including EGFR, PDGFR, src and insulin receptor and is involved in the negative regulation of receptor tyrosine kinase-coupled signaling pathways. The ligand binding of SIRP alpha to integrin-associated protein CD47 results in tyrosine kinase phosphorylation of immunoreceptor tyrosine-based inhibitory motifs (ITIMs) within the cytoplasmic region of SIRP alpha, which mediates the recruitment and activation of the tyrosine phosphatases SHP-1 and SHP-2. Ligation of SIRP alpha with CD47 has been demonstrated in several regulatory processes, including the inhibition of host cell phagocytosis by macrophages and the bi-directional activation of T cells and DCs. SIRP alpha has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones and cell adhesion, and plays a general role in different physiological and pathological processes. Cancer cells highly express CD47, which activates SIRP alpha and inhibits macrophage-mediated destruction of cancerous cell growth.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: CD172 alpha; CD172 antigen-like family member A; CD172a; Inhibitory receptor SHPS-1; MyD-1 antigen; protein tyrosine phosphatase, non-receptor type substrate 1; SHP substrate 1; SHPS-1; signal-regulatory protein alpha; Signal-regulatory protein alpha-1; Sirp-alpha-1; SIRPalpha; SIRPalpha2; Tyrosine-protein phosphatase non-receptor type substrate 1
Gene Aliases: BOS_13304; MYD-1; MYD1; PTPNS1; SHPS1; SIRP; SIRPA
UniProt ID: (Bovine) O46631
Entrez Gene ID: (Dog) 609452, (Bovine) 327666
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support