Invitrogen

CD18 (LFA-1 beta) Monoclonal Antibody (M18/2), FITC, eBioscience™

3 Published Figures

14 References

View all (89) CD18 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Published Figures (3)

CD18 (LFA-1 beta) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 3

CD18 (LFA-1 beta) Antibody (11-0181-82) in Flow

Frontiers in immunology 2021 - Published figure using CD18 (LFA-1 beta) monoclonal antibody (Product # 11-0181-82) in Flow Cytometry

CD18 (LFA-1 beta) Antibody in Immunohistochemistry (IHC)

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Product Details

11-0181-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Flow Cytometry (Flow)

1 µg/test

View 13 publications 13 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

M18/2

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_464960

Product Specific Information

Description: The M18/2 monoclonal antibody reacts with mouse CD18, the 90-95 kDa integrin beta 2 glycoprotein. CD18 is the common beta subunit that non-covalently associates with CD11a (alpha L), CD11b (alpha M) and CD11c (alpha X) to form LFA-1, Mac-1 and gp150/95. Heterodimers of CD18 and the a subunits are expressed broadly by all leukocytes with NK and T cells showing higher density of surface expression. Through the interactions of LFA-1, Mac-1 and gp150/95 with their counter-receptors, CD18 is involved in cellular adhesion and the immune response.

Applications Reported: The M18/2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The M18/2 antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD18, also known as the integrin beta 2 subunit, is a 90-95 kDa type I transmembrane protein expressed on all leukocytes. It heterodimerizes with the alpha chains of CD11a, CD11b, CD11c, and CD11d to form various leukocyte (beta2) integrins, which are crucial for cellular adhesion and signaling. The CD18/CD11a complex, known as lymphocyte function-associated antigen-1 (LFA-1), plays a significant role in cellular adhesion and inflammatory reactions. CD18 also forms heterodimers with CD11b (Mac-1, CR3), CD11c, and CD11d, contributing to the processes of cell adhesion and cell-surface mediated signaling. These integrins are essential for proper leukocyte migration and mediating intercellular contacts. The absence of CD18 leads to leukocyte adhesion deficiency type I (LAD1), a condition characterized by impaired leukocyte migration. Severe reduction in CD18 expression can result in the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin, which can mediate leukotoxin-induced cytolysis. Defects in the CD18 gene are the cause of LAD1, and two transcript variants encoding the CD18 protein have been identified. The integrin-mediated responses are often a composite of the functions of its individual subunits, highlighting the importance of CD18 in immune function and disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Cellular Membrane-Engineered Nanovesicles as a Three-Stage Booster to Target the Lesion Core.

Advanced materials (Deerfield Beach, Fla.)

Huang F,Du Y,Chen Y,Qiu Z,Wang Z,Fan Y,Shi Y,Li Z,Yang K,Cui W,Jin W

11-0181-82 was used in Flow Cytometry to demonstrate that a three-stage booster nanovesicle, constructed through the double emulsion-extrusion method and incorporating a neutrophil membrane, functional antibody, and gelled drug-loaded core, enables targeted delivery of catestatin for ischemic cardiomyopathy therapy.

Wed Nov 01 00:00:00 UTC 2023

Protein kinase D3 promotes neutrophil migration during viral infection.

Immunology and cell biology

Veazey JM,Wong GS,Eliseeva SI,Smyth TR,Chapman TJ,Lim K,Kim M,Georas SN

Published figure using CD18 (LFA-1 beta) monoclonal antibody (Product # 11-0181-82) in Flow Cytometry

Wed Feb 01 00:00:00 UTC 2023

Central Nervous System Barriers Impact Distribution and Expression of iNOS and Arginase-1 in Infiltrating Macrophages During Neuroinflammation.

Frontiers in immunology

Ivan DC,Walthert S,Locatelli G

Published figure using CD18 (LFA-1 beta) monoclonal antibody (Product # 11-0181-82) in Flow Cytometry

Mon Oct 25 00:00:00 UTC 2021

Cell-permeable transgelin-2 as a potent therapeutic for dendritic cell-based cancer immunotherapy.

Journal of hematology & oncology

Kim HR,Park JS,Park JH,Yasmin F,Kim CH,Oh SK,Chung IJ,Jun CD

11-0181 was used in Flow cytometry/Cell sorting to indicate that cell-permeable transgelin-2 have a potential clinical value as a cancer immunotherapy based on DCs.

Wed Mar 17 00:00:00 UTC 2021

Macrophage migration inhibitory factor promotes the migration of dendritic cells through CD74 and the activation of the Src/PI3K/myosin II pathway.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Ives A,Le Roy D,Théroude C,Bernhagen J,Roger T,Calandra T

11-0181-82 was used in Flow Cytometry to investigate whether migration inhibitory factor promoted the migration of Dendritic cells.

Sat May 01 00:00:00 UTC 2021

Interaction between ICAM1 in endothelial cells and LFA1 in T cells during the pathogenesis of experimental Parkinson's disease.

Experimental and therapeutic medicine

Li W,Chen S,Luo Y,Xia Y,Ma Q,Yao Q,Wu J

11-0181 was used in Flow cytometry/Cell sorting to investigate the infiltration of T cells in lesions in the central nervous system, in patients with and without Parkinson's Disease.

Sat Aug 01 00:00:00 UTC 2020

Sustained inflammation after pericyte depletion induces irreversible blood-retina barrier breakdown.

JCI insight

Ogura S,Kurata K,Hattori Y,Takase H,Ishiguro-Oonuma T,Hwang Y,Ahn S,Park I,Ikeda W,Kusuhara S,Fukushima Y,Nara H,Sakai H,Fujiwara T,Matsushita J,Ema M,Hirashima M,Minami T,Shibuya M,Takakura N,Kim P,Miyata T,Ogura Y,Uemura A

Published figure using CD18 (LFA-1 beta) monoclonal antibody (Product # 11-0181-82) in Immunohistochemistry

Thu Feb 09 00:00:00 UTC 2017

Antigen presenting cell abnormalities in the Cln3(-/-) mouse model of juvenile neuronal ceroid lipofuscinosis.

Biochimica et biophysica acta

Hersrud SL,Kovács AD,Pearce DA

11-0181 was used in Flow cytometry/Cell sorting to show that CLN3 deficiency alters antigen presenting cells, which can contribute to the autoimmune response in juvenile neuronal ceroid lipofuscinosis.

Fri Jul 01 00:00:00 UTC 2016

Glutaminyl cyclases as novel targets for the treatment of septic arthritis.

The Journal of infectious diseases

Hellvard A,Maresz K,Schilling S,Graubner S,Heiser U,Jonsson R,Cynis H,Demuth HU,Potempa J,Mydel P

11-0181 was used in Flow cytometry/Cell sorting to assess the antiarthritic efficiency of glutaminyl cyclase inhibitors.

Fri Mar 01 00:00:00 UTC 2013

Monoacylglycerol lipase (MAGL) inhibition attenuates acute lung injury in mice.

PloS one

Costola-de-Souza C,Ribeiro A,Ferraz-de-Paula V,Calefi AS,Aloia TP,Gimenes-Júnior JA,de Almeida VI,Pinheiro ML,Palermo-Neto J

11-0181 was used in Flow cytometry/Cell sorting to conclude that monoacylglycerol lipase inhibition and increased 2-Arachidonoylglycerol levels, produce anti-inflammatory effects in murine models of LPS-induced acute lung injury.

Tue Aug 05 00:00:00 UTC 2014

Impairment of the hematological response and interleukin-1β production in protein-energy malnourished mice after endotoxemia with lipopolysaccharide.

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas

Fock RA,Vinolo MA,Blatt SL,Borelli P

11-0181 was used in Flow cytometry/Cell sorting to determine to what extent protein-energy malnutrition could affect the haematologic response.

Sat Dec 01 00:00:00 UTC 2012

Dependence of proliferative vascular smooth muscle cells on CD98hc (4F2hc, SLC3A2).

The Journal of experimental medicine

Fogelstrand P,Féral CC,Zargham R,Ginsberg MH

11-0181 was used in Flow cytometry/Cell sorting to establish the importance of CD98hc in vascular smooth muscle cell function and survival.

Mon Oct 26 00:00:00 UTC 2009

Adsorbed serum albumin is permissive to macrophage attachment to perfluorocarbon polymer surfaces in culture.

Journal of biomedical materials research. Part A

Godek ML,Michel R,Chamberlain LM,Castner DG,Grainger DW

11-0181 was used in Flow cytometry/Cell sorting to investigate the mechanism by which macrophages bind to albumin-coated substrates and hydrophobic fluorinated surfaces in culture.

Sun Feb 01 00:00:00 UTC 2009

Galectin-9 suppresses tumor metastasis by blocking adhesion to endothelium and extracellular matrices.

Glycobiology

Nobumoto A,Nagahara K,Oomizu S,Katoh S,Nishi N,Takeshita K,Niki T,Tominaga A,Yamauchi A,Hirashima M

11-0181 was used in Flow cytometry/Cell sorting to investigate the efficiency and mechanism by which galectin-9 inhibits lung metastasis in experimental mouse models.

Mon Sep 01 00:00:00 UTC 2008

Bioinformatics

Protein Aliases: CD18; Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; Complement receptor C3 subunit beta; Integrin beta-2; lymphocyte function associated antigen 1; Mac-1 beta; macrophage antigen-1 beta

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Gene Aliases: 2E6; AI528527; Cd18; Itgb2; LAD; LCAMB; Lfa1; MF17

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UniProt ID: (Mouse) P11835

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Entrez Gene ID: (Mouse) 16414

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Function(s)

glycoprotein binding receptor activity protein binding protein kinase binding ICAM-3 receptor activity protein complex binding metal ion binding protein heterodimerization activity cell adhesion molecule binding

Process(es)

leukocyte migration involved in inflammatory response cell adhesion leukocyte cell-cell adhesion cell-matrix adhesion signal transduction integrin-mediated signaling pathway single organismal cell-cell adhesion natural killer cell activation neutrophil chemotaxis heterotypic cell-cell adhesion receptor clustering endothelial cell migration cellular extravasation positive regulation of nitric oxide biosynthetic process positive regulation of angiogenesis regulation of peptidyl-tyrosine phosphorylation activated T cell proliferation positive regulation of NF-kappaB transcription factor activity

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD18 (LFA-1 beta) Monoclonal Antibody (M18/2), FITC, eBioscience™

CD18 (LFA-1 beta) Antibody (11-0181-82) in Flow

Product Details

11-0181-82

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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