Invitrogen

CD18 (LFA-1 beta) Monoclonal Antibody (M18/2), FITC, eBioscience™

3 Published Figures

14 References

View all (82) CD18 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Published Figures (3)

CD18 (LFA-1 beta) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 3

CD18 (LFA-1 beta) Antibody (11-0181-82) in Flow

Frontiers in immunology 2021 - Published figure using CD18 (LFA-1 beta) monoclonal antibody (Product # 11-0181-82) in Flow Cytometry

CD18 (LFA-1 beta) Antibody in Immunohistochemistry (IHC)

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Product Details

11-0181-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Flow Cytometry (Flow)

1 µg/test

View 13 publications 13 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

M18/2

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_464960

Product Specific Information

Description: The M18/2 monoclonal antibody reacts with mouse CD18, the 90-95 kDa integrin beta 2 glycoprotein. CD18 is the common beta subunit that non-covalently associates with CD11a (alpha L), CD11b (alpha M) and CD11c (alpha X) to form LFA-1, Mac-1 and gp150/95. Heterodimers of CD18 and the a subunits are expressed broadly by all leukocytes with NK and T cells showing higher density of surface expression. Through the interactions of LFA-1, Mac-1 and gp150/95 with their counter-receptors, CD18 is involved in cellular adhesion and the immune response.

Applications Reported: The M18/2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The M18/2 antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD18 integrin beta 2 subunit is a 90 kDa type I transmembrane protein expressed on all leucocytes. CD18 can combine with integrin molecules CD11a-c to form heterodimers at the cell surface, and these heterodimers are known to participate in the process of cell adhesion as well as cell-surface mediated signaling. CD18 forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits, and these integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1, severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis. Defects in the CD18 gene are the cause of leukocyte adhesion deficiency type I (LAD1). Two transcript variants encoding the CD18 protein have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Cellular Membrane-Engineered Nanovesicles as a Three-Stage Booster to Target the Lesion Core.

Advanced materials (Deerfield Beach, Fla.)

Huang F,Du Y,Chen Y,Qiu Z,Wang Z,Fan Y,Shi Y,Li Z,Yang K,Cui W,Jin W

11-0181-82 was used in Flow Cytometry to demonstrate that a three-stage booster nanovesicle, constructed through the double emulsion-extrusion method and incorporating a neutrophil membrane, functional antibody, and gelled drug-loaded core, enables targeted delivery of catestatin for ischemic cardiomyopathy therapy.

Wed Nov 01 00:00:00 UTC 2023

Protein kinase D3 promotes neutrophil migration during viral infection.

Immunology and cell biology

Veazey JM,Wong GS,Eliseeva SI,Smyth TR,Chapman TJ,Lim K,Kim M,Georas SN

Published figure using CD18 (LFA-1 beta) monoclonal antibody (Product # 11-0181-82) in Flow Cytometry

Wed Feb 01 00:00:00 UTC 2023

Central Nervous System Barriers Impact Distribution and Expression of iNOS and Arginase-1 in Infiltrating Macrophages During Neuroinflammation.

Frontiers in immunology

Ivan DC,Walthert S,Locatelli G

Published figure using CD18 (LFA-1 beta) monoclonal antibody (Product # 11-0181-82) in Flow Cytometry

Mon Oct 25 00:00:00 UTC 2021

Cell-permeable transgelin-2 as a potent therapeutic for dendritic cell-based cancer immunotherapy.

Journal of hematology & oncology

Kim HR,Park JS,Park JH,Yasmin F,Kim CH,Oh SK,Chung IJ,Jun CD

11-0181 was used in Flow cytometry/Cell sorting to indicate that cell-permeable transgelin-2 have a potential clinical value as a cancer immunotherapy based on DCs.

Wed Mar 17 00:00:00 UTC 2021

Macrophage migration inhibitory factor promotes the migration of dendritic cells through CD74 and the activation of the Src/PI3K/myosin II pathway.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Ives A,Le Roy D,Théroude C,Bernhagen J,Roger T,Calandra T

11-0181-82 was used in Flow Cytometry to investigate whether migration inhibitory factor promoted the migration of Dendritic cells.

Sat May 01 00:00:00 UTC 2021

Interaction between ICAM1 in endothelial cells and LFA1 in T cells during the pathogenesis of experimental Parkinson's disease.

Experimental and therapeutic medicine

Li W,Chen S,Luo Y,Xia Y,Ma Q,Yao Q,Wu J

11-0181 was used in Flow cytometry/Cell sorting to investigate the infiltration of T cells in lesions in the central nervous system, in patients with and without Parkinson's Disease.

Sat Aug 01 00:00:00 UTC 2020

Sustained inflammation after pericyte depletion induces irreversible blood-retina barrier breakdown.

JCI insight

Ogura S,Kurata K,Hattori Y,Takase H,Ishiguro-Oonuma T,Hwang Y,Ahn S,Park I,Ikeda W,Kusuhara S,Fukushima Y,Nara H,Sakai H,Fujiwara T,Matsushita J,Ema M,Hirashima M,Minami T,Shibuya M,Takakura N,Kim P,Miyata T,Ogura Y,Uemura A

Published figure using CD18 (LFA-1 beta) monoclonal antibody (Product # 11-0181-82) in Immunohistochemistry

Thu Feb 09 00:00:00 UTC 2017

Antigen presenting cell abnormalities in the Cln3(-/-) mouse model of juvenile neuronal ceroid lipofuscinosis.

Biochimica et biophysica acta

Hersrud SL,Kovács AD,Pearce DA

11-0181 was used in Flow cytometry/Cell sorting to show that CLN3 deficiency alters antigen presenting cells, which can contribute to the autoimmune response in juvenile neuronal ceroid lipofuscinosis.

Fri Jul 01 00:00:00 UTC 2016

Glutaminyl cyclases as novel targets for the treatment of septic arthritis.

The Journal of infectious diseases

Hellvard A,Maresz K,Schilling S,Graubner S,Heiser U,Jonsson R,Cynis H,Demuth HU,Potempa J,Mydel P

11-0181 was used in Flow cytometry/Cell sorting to assess the antiarthritic efficiency of glutaminyl cyclase inhibitors.

Fri Mar 01 00:00:00 UTC 2013

Monoacylglycerol lipase (MAGL) inhibition attenuates acute lung injury in mice.

PloS one

Costola-de-Souza C,Ribeiro A,Ferraz-de-Paula V,Calefi AS,Aloia TP,Gimenes-Júnior JA,de Almeida VI,Pinheiro ML,Palermo-Neto J

11-0181 was used in Flow cytometry/Cell sorting to conclude that monoacylglycerol lipase inhibition and increased 2-Arachidonoylglycerol levels, produce anti-inflammatory effects in murine models of LPS-induced acute lung injury.

Tue Aug 05 00:00:00 UTC 2014

Impairment of the hematological response and interleukin-1β production in protein-energy malnourished mice after endotoxemia with lipopolysaccharide.

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas

Fock RA,Vinolo MA,Blatt SL,Borelli P

11-0181 was used in Flow cytometry/Cell sorting to determine to what extent protein-energy malnutrition could affect the haematologic response.

Sat Dec 01 00:00:00 UTC 2012

Dependence of proliferative vascular smooth muscle cells on CD98hc (4F2hc, SLC3A2).

The Journal of experimental medicine

Fogelstrand P,Féral CC,Zargham R,Ginsberg MH

11-0181 was used in Flow cytometry/Cell sorting to establish the importance of CD98hc in vascular smooth muscle cell function and survival.

Mon Oct 26 00:00:00 UTC 2009

Adsorbed serum albumin is permissive to macrophage attachment to perfluorocarbon polymer surfaces in culture.

Journal of biomedical materials research. Part A

Godek ML,Michel R,Chamberlain LM,Castner DG,Grainger DW

11-0181 was used in Flow cytometry/Cell sorting to investigate the mechanism by which macrophages bind to albumin-coated substrates and hydrophobic fluorinated surfaces in culture.

Sun Feb 01 00:00:00 UTC 2009

Galectin-9 suppresses tumor metastasis by blocking adhesion to endothelium and extracellular matrices.

Glycobiology

Nobumoto A,Nagahara K,Oomizu S,Katoh S,Nishi N,Takeshita K,Niki T,Tominaga A,Yamauchi A,Hirashima M

11-0181 was used in Flow cytometry/Cell sorting to investigate the efficiency and mechanism by which galectin-9 inhibits lung metastasis in experimental mouse models.

Mon Sep 01 00:00:00 UTC 2008

Bioinformatics

Protein Aliases: CD18; Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; Complement receptor C3 subunit beta; Integrin beta-2; lymphocyte function associated antigen 1; Mac-1 beta; macrophage antigen-1 beta

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Gene Aliases: 2E6; AI528527; Cd18; Itgb2; LAD; LCAMB; Lfa1; MF17

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UniProt ID: (Mouse) P11835

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Entrez Gene ID: (Mouse) 16414

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Function(s)

glycoprotein binding receptor activity protein binding protein kinase binding ICAM-3 receptor activity protein complex binding metal ion binding protein heterodimerization activity cell adhesion molecule binding

Process(es)

leukocyte migration involved in inflammatory response cell adhesion leukocyte cell-cell adhesion cell-matrix adhesion signal transduction integrin-mediated signaling pathway single organismal cell-cell adhesion natural killer cell activation neutrophil chemotaxis heterotypic cell-cell adhesion receptor clustering endothelial cell migration cellular extravasation positive regulation of nitric oxide biosynthetic process positive regulation of angiogenesis regulation of peptidyl-tyrosine phosphorylation activated T cell proliferation positive regulation of NF-kappaB transcription factor activity

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD18 (LFA-1 beta) Monoclonal Antibody (M18/2), FITC, eBioscience™

CD18 (LFA-1 beta) Antibody (11-0181-82) in Flow

Product Details

11-0181-82

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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