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          • Primary Antibodies ›
          • CXCR3 Antibodies
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          Invitrogen

          CD183 (CXCR3) Monoclonal Antibody (CXCR3-173), Functional Grade, eBioscience™

          21 Published Figures
          35 References
          View all (61) CXCR3 antibodies

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          Datasheet
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          Datasheet
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          Cite CD183 (CXCR3) Monoclonal Antibody (CXCR3-173), Functional Grade, eBioscience™

          • Antibody Testing Data (1)
          • Published Figures (21)
          CD183 (CXCR3) Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          CD183 (CXCR3) Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 22

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          CD183 (CXCR3) Antibody (16-1831-025) in Flow

          Staining of C57Bl/6 splenocytes with Anti-Mouse CD8a APC (Product # 17-0081-82) and 0.25 µg of Armenian Hamster IgG Isotype Control Functional Grade Purified (Product # 16-4888-81) (left) or 0.25 µg of Anti-Mouse CD183 (CXCR3) Functional Grade Purified (right) followed by Anti-Armenian Hamster IgG PE (Product # 12-4112-83).Total viable cells were used for analysis. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          CD183 (CXCR3) Antibody in Flow Cytometry (Flow)
          CD183 (CXCR3) Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          CD183 (CXCR3) Antibody in Flow Cytometry (Flow)
          CD183 (CXCR3) Antibody in Flow Cytometry (Flow)
          CD183 (CXCR3) Antibody in Flow Cytometry (Flow)
          CD183 (CXCR3) Antibody in Flow Cytometry (Flow)
          CD183 (CXCR3) Antibody in Flow Cytometry (Flow)
          CD183 (CXCR3) Antibody in Flow Cytometry (Flow)
          CD183 (CXCR3) Antibody in Flow Cytometry (Flow)
          CD183 (CXCR3) Antibody in Flow Cytometry (Flow)
          CD183 (CXCR3) Antibody in Flow Cytometry (Flow)
          CD183 (CXCR3) Antibody in Flow Cytometry (Flow)
          CD183 (CXCR3) Antibody in Flow Cytometry (Flow)
          CD183 (CXCR3) Antibody in Flow Cytometry (Flow)
          CD183 (CXCR3) Antibody in Flow Cytometry (Flow)
          CD183 (CXCR3) Antibody in Flow Cytometry (Flow)
          CD183 (CXCR3) Antibody in Flow Cytometry (Flow)
          CD183 (CXCR3) Antibody in Flow Cytometry (Flow)
          CD183 (CXCR3) Antibody in Flow Cytometry (Flow)
          CD183 (CXCR3) Antibody in Flow Cytometry (Flow)
          CD183 (CXCR3) Antibody in Flow Cytometry (Flow)
          CD183 (CXCR3) Antibody in Flow Cytometry (Flow)

          Product Details

          16-1831-025

          Applications
          Tested Dilution
          Publications

          Immunohistochemistry (IHC)

          -
          View 2 publications 2 publications

          Immunocytochemistry (ICC/IF)

          -
          View 1 publication 1 publication

          Flow Cytometry (Flow)

          0.5 µg/test
          View 28 publications 28 publications

          Neutralization (Neu)

          -
          View 1 publication 1 publication

          Functional Assay (Functional)

          Assay-Dependent
          View 3 publications 3 publications
          Product Specifications

          Published species

          Mouse

          Host/Isotype

          Armenian hamster / IgG

          Recommended Isotype Control

          Armenian Hamster IgG Isotype Control (eBio299Arm), Functional Grade, eBioscience™

          Class

          Monoclonal

          Type

          Antibody

          Clone

          CXCR3-173

          Conjugate

          Functional Grade Functional Grade Functional Grade
          • APC 
          • Biotin 
          • FITC
          • NovaFluor Blue 610-70S
          • NovaFluor Blue 660-120S
          • NovaFluor Blue 690
          • NovaFluor Blue 800
          • NovaFluor Yellow 810
          • PE 
          • PE-Cyanine7
          • PE-eFluor 610
          • PerCP-Cyanine5.5
          • Super Bright 436
          • Super Bright 600
          • Request custom conjugation

          Form

          Liquid

          Concentration

          1 mg/mL

          Storage conditions

          4°C

          Shipping conditions

          Wet ice

          RRID

          AB_2865908

          Product Specific Information

          Description: The monoclonal antibody CXCR3-173 recognizes mouse CD183 also known as CXCR3. CD183 is a seven transmembrane G-protein liked chemokine receptor which binds three ligands; CXCL9 (mig), CXCL10 (IP-10)and CXCL11 (ITAC). CD183 as been shown to play a role in CD4 T cell responses to grafts. CXCR3 knockout mice have compromised allograft rejection responses. Expression is found on NK cells, a subset of T lymphocytes and a subset of Tregs as well as preferential expression on Th1-polarized cells.



          The antibody CXCR3-173 has been shown to affect chemotaxis in response to ligand. The presence of ligand eliminates staining with the antibody. In vivo addition of the antibody delays cardiac and pancreatic islet allograft rejection.

          Applications Reported: This CXCR3-173 antibody has been reported for use in flow cytometric analysis.

          Applications Tested: This CXCR3-173 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Storage and handling: Use in a sterile environment.

          Filtration: 0.2 µm post-manufacturing filtered.

          Purity: Greater than 90%, as determined by SDS-PAGE.

          Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

          Aggregation: Less than 10%, as determined by HPLC.

          Target Information

          CD183, also known as CXCR3, is a seven-transmembrane G protein-coupled chemokine receptor that binds CXCL9 (Mig), CXCL10 (IP-10), and CXCL11 (I-TAC), which are part of the CXC chemokine subfamily. CD183 plays a crucial role in leukocyte traffic, influencing integrin activation, cytoskeletal changes, and chemotactic migration. It is expressed on NK cells, subsets of T lymphocytes, regulatory T cells (Tregs), and is preferentially found on Th1-polarized cells. CD183 is prominently expressed in effector/memory T cells and T cells in inflamed tissues, contributing to CD4 T cell responses to grafts, as evidenced by compromised allograft rejection in CXCR3 knockout mice. Chemokine binding induces rapid, short-lived cellular responses due to receptor internalization, with responsiveness restored after receptor recycling. Inhibition by Bordetella pertussis toxin suggests coupling with Gi subclass G proteins. The production of IP-10, Mig, and I-TAC in inflammatory lesions indicates CD183 role in recruiting inflammatory cells, making it a target for developing antagonists to treat inflammatory diseases. Multiple transcript variants encoding different isoforms of CD183 have been identified.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          It has to be done as per old AB suggested Products section.
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