Invitrogen

CD183 (CXCR3) Monoclonal Antibody (CXCR3-173), PE-eFluor™ 610, eBioscience™

14 Published Figures

9 References

View all (55) CXCR3 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (14)

CD183 (CXCR3) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 15

CD183 (CXCR3) Antibody (61-1831-82) in Flow

C57BL/6 mouse splenocytes were stained with CD4 Monoclonal Antibody, APC (Product # 17-0042-82) and 0.25 µg of Armenian Hamster IgG Isotype Control, PE-eFluor 610 (Product # 61-4888-82) (left) or 0.25 µg of CD183 (CXCR3) Monoclonal Antibody, PE-eFluor 610 (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

61-1831-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 9 publications 9 publications

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), PE-eFluor™ 610, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

CXCR3-173

Conjugate

PE-eFluor™ 610 PE-eFluor™ 610 PE-eFluor™ 610

Excitation/Emission Max

565/606 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2802385

Product Specific Information

Description: The monoclonal antibody CXCR3-173 recognizes mouse CD183 also known as CXCR3. CD183 is a seven transmembrane G-protein liked chemokine receptor which binds three ligands; CXCL9 (mig), CXCL10 (IP-10)and CXCL11 (ITAC). CD183 as been shown to play a role in CD4 T cell responses to grafts. CXCR3 knockout mice have compromised allograft rejection responses. Expression is found on NK cells, a subset of T lymphocytes and a subset of Tregs as well as preferential expression on Th1-polarized cells.

Applications Reported: This CXCR3-173 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This CXCR3-173 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PE-eFluor 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red). Please make sure that your instrument is capable of detecting this fluorochrome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Target Information

This gene encodes a G protein-coupled receptor with selectivity for three chemokines, termed IP10 (interferon-g-inducible 10 kDa protein), Mig (monokine induced by interferon-g) and I-TAC (interferon-inducible T cell a-chemoattractant). IP10, Mig and I-TAC belong to the structural subfamily of CXC chemokines, in which a single amino acid residue separates the first two of four highly conserved Cys residues. Binding of chemokines to this protein induces cellular responses that are involved in leukocyte traffic, most notably integrin activation, cytoskeletal changes and chemotactic migration. Inhibition by Bordetella pertussis toxin suggests that heterotrimeric G protein of the Gi-subclass couple to this protein. Signal transduction has not been further analyzed but may include the same enzymes that were identified in the signaling cascade induced by other chemokine receptors. As a consequence of chemokine-induced cellular desensitization (phosphorylation-dependent receptor internalization), cellular responses are typically rapid and short in duration. Cellular responsiveness is restored after dephosphorylation of intracellular receptors and subsequent recycling to the cell surface. This gene is prominently expressed in in vitro cultured effector/memory T cells, and in T cells present in many types of inflamed tissues. In addition, IP10, Mig and I-TAC are commonly produced by local cells in inflammatory lesion, suggesting that this gene and its chemokines participate in the recruitment of inflammatory cells. Therefore, this protein is a target for the development of small molecular weight antagonists, which may be used in the treatment of diverse inflammatory diseases. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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