Invitrogen

CD184 (CXCR4) Monoclonal Antibody (2B11), Biotin, eBioscience™

20 Published Figures

24 References

View all (70) CXCR4 (CD184) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (20)

CD184 (CXCR4) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 21

CD184 (CXCR4) Antibody (13-9991-82) in Flow

Staining of C57Bl/6 thymocytes with Anti-Mouse CD4 eFluor® 450 (Product # 48-0042-82) and 0.5 µg of Rat IgG2b Isotype Control Biotin (Product # 13-4031-82) (left) or 0.5 µg of A nti-Mouse CD184 (CXCR4) (right) followed by Streptavidin APC (Product # 17-4314-82). Total viable cells were used for analysis.

CD184 (CXCR4) Antibody in Western Blot (WB)

CD184 (CXCR4) Antibody in Immunocytochemistry (ICC/IF)

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Product Details

13-9991-82

Applications

Tested Dilution

Publications

Western Blot (WB)

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Flow Cytometry (Flow)

1 µg/test

View 19 publications 19 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

2B11

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10609202

Product Specific Information

Description: The 2B11 monoclonal antibody reacts with mouse and human CXCR4, also known as CD184, Fusin, LESTR, or HUMSTR. Fusin is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains, and functions as a coreceptor for X4 HIV-1 entry into CD4+ cells. In addition it has been shown to play a role in lymphocyte migration. The ligand for CXCR4 is SDF1. CXCR4 is required for maintenance of granulocyte progenitors in the bone marrow and essential for B cell development. Expression of CXCR4 is different between mice and human. In the thymus CXCR4 is restricted to CD4+CD8+ cells while in the spleen predominant expression is found on B lymphocytes.

The monoclonal antibody 2B11 recognizes the amino terminus of CXCR4 and has been reported to have functional activity; it does not block SDF1a but causes internalization of the receptor.

Applications Reported: This 2B11 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 2B11 antibody has been tested by flow cytometric of mouse thymocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CXCR4 (Fusin, LESTR, HUMSTR) is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains, and functions as a co-receptor for X4 HIV-1 entry into CD4+ cells. CXCR4 has been proved to be the co-receptor for HIV-2's binding to CD4 through envelope glycoprotein gp 120 and promotes Env-mediated fusion of the virus. In other cases, CXCR4 can even function as the only receptor for HIV-2's binding to the CD4 host cells. CXCR4 is the receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. CXCR4 acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. Also, CXCR4 is involved in hematopoiesis and in cardiac ventricular septum formation, and plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. In the CNS, CXCR4 could mediate hippocampal-neuron survival and is involved in cerebellar development. CXCR4 binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells. The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV biding sites.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Designing Synthetic, Sulfated Glycosaminoglycan Mimetics That Are Orally Bioavailable and Exhibiting In Vivo Anticancer Activity.

Journal of medicinal chemistry

Morla S,Ravikumar O,O'Hara C,Boothello R,Vera A,Abdelfadiel EI,Fayyad R,Afosah DK,Sharon C,Fernandez L,Shah SA,Patel BB,Desai UR

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 13-9991-82) in Flow Cytometry

Thu Jan 26 00:00:00 UTC 2023

PHGDH is required for germinal center formation and is a therapeutic target in MYC-driven lymphoma.

The Journal of clinical investigation

D'Avola A,Legrave N,Tajan M,Chakravarty P,Shearer RL,King HW,Kluckova K,Cheung EC,Clear AJ,Gunawan AS,Zhang L,James LK,MacRae JI,Gribben JG,Calado DP,Vousden KH,Riches JC

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 13-9991-82) in Flow Cytometry

Mon May 02 00:00:00 UTC 2022

Krüppel-like factor 2 controls IgA plasma cell compartmentalization and IgA responses.

Mucosal immunology

Wittner J,Schulz SR,Steinmetz TD,Berges J,Hauke M,Channell WM,Cunningham AF,Hauser AE,Hutloff A,Mielenz D,Jäck HM,Schuh W

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 13-9991-82) in Flow Cytometry

Fri Apr 01 00:00:00 UTC 2022

Smc3 dosage regulates B cell transit through germinal centers and restricts their malignant transformation.

Nature immunology

Rivas MA,Meydan C,Chin CR,Challman MF,Kim D,Bhinder B,Kloetgen A,Viny AD,Teater MR,McNally DR,Doane AS,Béguelin W,Fernández MTC,Shen H,Wang X,Levine RL,Chen Z,Tsirigos A,Elemento O,Mason CE,Melnick AM

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 13-9991-82) in Flow Cytometry

Mon Feb 01 00:00:00 UTC 2021

SARS-CoV-2 mRNA Vaccines Foster Potent Antigen-Specific Germinal Center Responses Associated with Neutralizing Antibody Generation.

Immunity

Lederer K,Castaño D,Gómez Atria D,Oguin TH,Wang S,Manzoni TB,Muramatsu H,Hogan MJ,Amanat F,Cherubin P,Lundgreen KA,Tam YK,Fan SHY,Eisenlohr LC,Maillard I,Weissman D,Bates P,Krammer F,Sempowski GD,Pardi N,Locci M

13-9991 was used in Flow cytometry/Cell sorting to demonstrated that a single immunization with SARS-CoV-2 mRNA, but not with the recombinant protein vaccine, elicited potent SARS-CoV-2-specific GC B and T follicular helper (Tfh) cell responses as well as LLPCs and MBCs.

Tue Dec 15 00:00:00 UTC 2020

SARS-CoV-2 mRNA Vaccines Foster Potent Antigen-Specific Germinal Center Responses Associated with Neutralizing Antibody Generation.

Immunity

Tue Dec 15 00:00:00 UTC 2020

PECAM1 Combines With CXCR4 to Trigger Inflammatory Cell Infiltration and Pulpitis Progression Through Activating the NF-κB Signaling Pathway.

Frontiers in cell and developmental biology

Liu Y,Zhang Z,Li W,Tian S

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 13-9991-82) in Western Blot

Tue Jan 12 00:00:00 UTC 2021

Inhibition of Migration, Invasion and Drug Resistance of Pancreatic Adenocarcinoma Cells - Role of Snail, Slug and Twist and Small Molecule Inhibitors.

OncoTargets and therapy

Kaşıkcı E,Aydemir E,Bayrak ÖF,Şahin F

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 13-9991-82) in Flow Cytometry

Tue Jul 30 00:00:00 UTC 2024

Metabolic Imaging Reveals a Unique Preference of Symmetric Cell Division and Homing of Leukemia-Initiating Cells in an Endosteal Niche.

Cell metabolism

Hao X,Gu H,Chen C,Huang D,Zhao Y,Xie L,Zou Y,Shu HS,Zhang Y,He X,Lai X,Zhang X,Zhou BO,Zhang CC,Chen GQ,Yu Z,Yang Y,Zheng J

13-9991 was used in Flow cytometry/Cell sorting to provide a unique angle for the study of metabolisms in stem cells, and may lead to development of novel strategies for cancer treatment.

Tue Apr 02 00:00:00 UTC 2019

Enhanced germinal center reaction by targeting vaccine antigen to major histocompatibility complex class II molecules.

NPJ vaccines

Andersen TK,Huszthy PC,Gopalakrishnan RP,Jacobsen JT,Fauskanger M,Tveita AA,Grødeland G,Bogen B

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 13-9991-82) in Flow Cytometry

Thu Oct 05 00:00:00 UTC 2023

Skeletal Muscle Stem Cells from PSC-Derived Teratomas Have Functional Regenerative Capacity.

Cell stem cell

Chan SS,Arpke RW,Filareto A,Xie N,Pappas MP,Penaloza JS,Perlingeiro RCR,Kyba M

13-9991 was used in Flow cytometry/Cell sorting to show that teratomas formed in adult skeletal muscle differentiate in vivo to produce large numbers of α7-Integrin+ VCAM-1+ myogenic progenitors.

Thu Jul 05 00:00:00 UTC 2018

Precardiac organoids form two heart fields via Bmp/Wnt signaling.

Nature communications

Andersen P,Tampakakis E,Jimenez DV,Kannan S,Miyamoto M,Shin HK,Saberi A,Murphy S,Sulistio E,Chelko SP,Kwon C

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 13-9991-82) in Immunofluorescence

Tue Aug 07 00:00:00 UTC 2018

A Functional Bioluminescent Zebrafish Screen for Enhancing Hematopoietic Cell Homing.

Stem cell reports

Astuti Y,Kramer AC,Blake AL,Blazar BR,Tolar J,Taisto ME,Lund TC

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 13-9991-82) in Flow Cytometry

Tue Jan 10 00:00:00 UTC 2017

Non-genotoxic conditioning for hematopoietic stem cell transplantation using a hematopoietic-cell-specific internalizing immunotoxin.

Nature biotechnology

Palchaudhuri R,Saez B,Hoggatt J,Schajnovitz A,Sykes DB,Tate TA,Czechowicz A,Kfoury Y,Ruchika F,Rossi DJ,Verdine GL,Mansour MK,Scadden DT

13-9991 was used in Flow cytometry/Cell sorting to show that CD45-SAP efficiently conditions immunocompetent mice for autologous HSCT in a way that minimises toxicity and promotes rapid immunological recovery.

Fri Jul 01 00:00:00 UTC 2016

Optochemokine Tandem for Light-Control of Intracellular Ca2.

PloS one

Feldbauer K,Schlegel J,Weissbecker J,Sauer F,Wood PG,Bamberg E,Terpitz U

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 13-9991-82) in Immunofluorescence

Tue Jun 20 00:00:00 UTC 2017

Induced Pluripotent Stem Cells derived Muscle Progenitors Effectively Mitigate Muscular Dystrophy through Restoring the Dystrophin Distribution.

Journal of stem cell research & therapy

Cai WF,Huang W,Wang L,Wang JP,Zhang L,Ashraf M,Wu S,Wang Y

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 13-9991-82) in Flow Cytometry

Wed Sep 30 00:00:00 UTC 2020

Inhibition of insulin-like growth factor receptor/AKT/mammalian target of rapamycin axis targets colorectal cancer stem cells by attenuating mevalonate-isoprenoid pathway in vitro and in vivo.

Oncotarget

Sharon C,Baranwal S,Patel NJ,Rodriguez-Agudo D,Pandak WM,Majumdar AP,Krystal G,Patel BB

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 13-9991-82) in Flow Cytometry

Sat Jun 20 00:00:00 UTC 2015

Intravenous transplantation of very small embryonic like stem cells in treatment of diabetes mellitus.

Avicenna journal of medical biotechnology

Abouzaripour M,Ragerdi Kashani I,Pasbakhsh P,Atlasy N

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 13-9991-82) in Flow Cytometry

Thu Apr 30 00:00:00 UTC 2015

Fibrocytes in the fibrotic lung: altered phenotype detected by flow cytometry.

Frontiers in pharmacology

Reese C,Lee R,Bonner M,Perry B,Heywood J,Silver RM,Tourkina E,Visconti RP,Hoffman S

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 13-9991-82) in Flow Cytometry

Mon Jul 07 00:00:00 UTC 2014

Peyer's patch inducer cells play a leading role in the formation of B and T cell zone architecture.

Journal of immunology (Baltimore, Md. : 1950)

Nakagawa R,Togawa A,Nagasawa T,Nishikawa S

13-9991 was used in Flow cytometry/Cell sorting to analyse evidence that CD3(-)IL-7Rα(+) PP inducer cells are involved in the formation of B and T cell zones in neonatal mice.

Mon Apr 01 00:00:00 UTC 2013

Intramuscular VEGF activates an SDF1-dependent progenitor cell cascade and an SDF1-independent muscle paracrine cascade for cardiac repair.

American journal of physiology. Heart and circulatory physiology

Zisa D,Shabbir A,Mastri M,Taylor T,Aleksic I,McDaniel M,Suzuki G,Lee T

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 13-9991-82) in Immunofluorescence

Thu Dec 01 00:00:00 UTC 2011

Intramuscular VEGF activates an SDF1-dependent progenitor cell cascade and an SDF1-independent muscle paracrine cascade for cardiac repair.

American journal of physiology. Heart and circulatory physiology

Zisa D,Shabbir A,Mastri M,Taylor T,Aleksic I,McDaniel M,Suzuki G,Lee T

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 13-9991-82) in Immunofluorescence

Thu Dec 01 00:00:00 UTC 2011

Intramuscular VEGF repairs the failing heart: role of host-derived growth factors and mobilization of progenitor cells.

American journal of physiology. Regulatory, integrative and comparative physiology

Zisa D,Shabbir A,Mastri M,Suzuki G,Lee T

Published figure using CD184 (CXCR4) monoclonal antibody (Product # 13-9991-82) in Flow Cytometry

Sun Nov 01 00:00:00 UTC 2009

The murine chemokine receptor CXCR4 is tightly regulated during T cell development and activation.

Journal of leukocyte biology

Schabath R,Müller G,Schubel A,Kremmer E,Lipp M,Förster R

Wed Dec 01 00:00:00 UTC 1999

The murine chemokine receptor CXCR4 is tightly regulated during T cell development and activation.

Journal of leukocyte biology

Schabath R,Müller G,Schubel A,Kremmer E,Lipp M,Förster R

Wed Dec 01 00:00:00 UTC 1999

Bioinformatics

Protein Aliases: C Cmotif chemokine; C X C motif chemokine; C-X-C chemokine receptor type 4; CC motif chemokine; CCmotif chemokine; CD antigen CD184; CD184; chemokine (C-X-C) receptor 4; chemokine receptor 4; CXC; CXC motif chemokine; CXC-R4; CXCR-4; Fusin; HUMSTR; LESTR; Leukocyte-derived seven transmembrane domain receptor; leukocyte-expressed seven-transmembrane-domain; PB-CKR; Pre-B-cell-derived chemokine receptor; SDF-1 receptor; Stromal cell-derived factor 1 receptor

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Gene Aliases: b2b220Clo; CD184; Cmkar4; Cxcr4; LESTR; PB-CKR; PBSF/SDF-1; Sdf1r

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UniProt ID: (Mouse) P70658

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Entrez Gene ID: (Mouse) 12767

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Function(s)

actin binding signal transducer activity G-protein coupled receptor activity chemokine receptor activity protein binding drug binding C-X-C chemokine receptor activity cytokine binding ubiquitin protein ligase binding myosin light chain binding ubiquitin binding

Process(es)

patterning of blood vessels ameboidal-type cell migration neuron migration epithelial cell development chemotaxis signal transduction G-protein coupled receptor signaling pathway germ cell development nervous system development brain development motor neuron axon guidance germ cell migration organ morphogenesis cell migration calcium-mediated signaling telencephalon cell migration regulation of cell migration T cell proliferation neutrophil activation regulation of programmed cell death myelin maintenance positive regulation of oligodendrocyte differentiation regulation of chemotaxis regulation of calcium ion transport cardiac muscle contraction chemokine-mediated signaling pathway cellular response to cytokine stimulus

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD184 (CXCR4) Monoclonal Antibody (2B11), Biotin, eBioscience™

CD184 (CXCR4) Antibody (13-9991-82) in Flow

Product Details

13-9991-82

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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