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Invitrogen
CD185 (CXCR5) Monoclonal Antibody (87.1), PE, eBioscience™
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FIGURE: 1 / 1
CD185 (CXCR5) Antibody (12-1859-42) in Flow
Staining of normal rhesus peripheral blood cells with Anti-Human CD20 APC (Product # 17-0209-42) and Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or Anti-Non-Human Primate CD185 (CXCR5) PE (right). Cells in the lymphocyte gate were used for analysis.
Product Details
12-1859-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Non-human primate,
Rhesus monkey
Published species
Cynomolgus monkey
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
87.1
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_11218708
Product Specific Information
Description: This 87.1 monoclonal antibody reacts with non-human primate (specifically rhesus) CD185. CD185, which is also known as C-X-C chemokine receptor 5 (CXCR5) and Burkitt lymphoma receptor 1 (BLR1), is a seven transmembrane G protein-coupled receptor originally identified in Burkitt's lymphoma. In peripheral blood, CD185 is expressed on B cells, CD4+ T cells (but not Th1 or Th2 cells), and a subpopulation of memory (CD45RO+) T cells. CD185+ circulating T cells are in a resting state and migrate to the lymph nodes due to CCR7 and CD62L expression. In tonsils, CD185 is expressed on nearly all CD4+ cells along with CD45RO and the activation markers CD69 and ICOS. Tonsillar CD185+ cells have been shown to induce antibody production when co-cultured with B cells, thus supporting their role in providing B cell help. Furthermore, this chemokine receptor plays a critical role in lymphocyte trafficking, in particular CXCL13-induced T cell migration into the B cell follicles of germinal centers. Thus, CD185 is an established marker of follicular helper T cells (Tfh). In rhesus macaques, infection with simian immunodeficiency virus (SIV) has been reported to increase the number of germinal centers and Tfh cells within the lymph nodes.
Refer to the rhesus reactive alternative clone MU5UBEE (Product # 12-9185) for identification of CXCR5 on T cells in addition to B cells.
Applications Reported: This 87.1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 87.1 antibody has been pre-titrated and tested by flow cytometric analysis of normal rhesus peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD185, also known as CXCR5 or Burkitt lymphoma receptor 1 (BLR1), is a seven-transmembrane G protein-coupled receptor in the CXC chemokine receptor family. It plays a crucial role in lymphocyte trafficking, particularly in T cell migration into B cell follicles of germinal centers in response to CXCL13, making it a key marker of follicular helper T cells. In peripheral blood, CXCR5 is expressed on B cells, CD4+ T cells (excluding Th1 and Th2 cells), and a subset of memory (CD45RO+) T cells. These CD185+ T cells are in a resting state and migrate to lymph nodes due to CCR7 and CD62L expression. In tonsils, CD185 is found on nearly all CD4+ cells, along with activation markers like CD69 and ICOS, and can induce antibody production when co-cultured with B cells. CXCR5 is expressed in lymphatic tissues such as lymph node follicles and the spleen, and is also found in human blood and salivary glands, as well as various animal tissues. It is essential for B cell migration and is part of the larger family of G protein-coupled receptors (GPCRs), which have seven membrane-spanning helices and are involved in numerous signaling pathways. GPCRs, including CXCR5, are regulated by various ligands that affect their conformation and signaling through G-proteins and downstream effectors. The regulation of GPCRs, including their deactivation, has significant implications for cellular functions and is a major focus of pharmacological research, leading to the development of drugs like Zyprexa and Claritin for mental health and allergy treatments.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Blr-1; Burkitt lymphoma receptor 1; C Cmotif chemokine; C X C motif chemokine; CC motif chemokine; CCmotif chemokine; CD185 antigen; chemokine (C-X-C motif) receptor 5; CXC; CXC motif chemokine; Fusin; GPR9; HUMSTR; LESTR
Gene Aliases: BLR1; EGK_06966
Entrez Gene ID: (Rhesus monkey) 701792
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