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Invitrogen
CD19 Monoclonal Antibody (6D5), Qdot™ 655
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FIGURE: 1 / 1
CD19 Antibody (Q10379) in Flow
Log fluorescence intensity profiles of mouse splenocytes gated on lymphocytes and analyzed on a BD LSR II flow cytometer (BD Biosciences, San Jose, CA) using 405 nm excitation and the specified emission filters. The black line represents cells stained with anti-mouse CD19 antibody conjugate and the gray line represents unstained cells. Note: Flow cytometric data shown may not necessarily have been generated using the enclosed lot of reagent. For this reason, and due to differences in flow cytometers and cytometer settings, results may vary... View More
Product Details
Q10379
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
6D5
Immunogen
mouse CD19 antigen
Conjugate
Excitation/Emission Max
300/654 nm
View spectra
Form
Liquid
Purification
Affinity chromatography
Storage buffer
0.05M borate, pH 8.3, with 1M betaine
Contains
0.05% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10563403
Target Information
CD19 is a member of the immunoglobulin superfamily, characterized by two Ig-like domains, and is expressed on B cells throughout all stages of development, excluding terminally differentiated plasma cells. It is also expressed on follicular dendritic cells and has been observed on myeloid leukemia cells, particularly those of monocytic lineage. CD19 is considered the earliest and broadest B cell-restricted antigen, and its expression is found in all B cell precursor leukemias. CD19 forms a multimolecular complex with CD21, CD81, Leu13, MHC class II, and the B cell receptor (BCR), playing a crucial role in B cell signaling. As a signal-amplifying coreceptor for the BCR, CD19 lowers the threshold for antigen receptor-dependent stimulation, allowing B cells to respond specifically and sensitively to various antigens through low-affinity antigen receptors. Signaling through CD19 induces tyrosine phosphorylation, calcium flux, and proliferation of B cells. Beyond its role as a BCR coreceptor, CD19 can also signal independently of BCR co-ligation, serving as a central regulatory component upon which multiple signaling pathways converge. This makes CD19 an important functional regulator of both normal and malignant B cell proliferation. Mutations in the CD19 gene can result in hypogammaglobulinemia, a condition characterized by low levels of immunoglobulins, while CD19 overexpression can lead to B cell hyperactivity. CD19 is expressed on 100% of peripheral B cells, as defined by the expression of kappa or lambda light chains, underscoring its significance in B cell function and immune regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B-lymphocyte antigen CD19; CD19; Differentiation antigen CD19; Leu-12
Gene Aliases: AW495831; Cd19
UniProt ID: (Mouse) P25918
Entrez Gene ID: (Mouse) 12478
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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