Invitrogen

CD195 (CCR5) Monoclonal Antibody (HM-CCR5 (7A4)), PerCP-eFluor™ 710, eBioscience™

2 Published Figures

5 References

View all (44) CCR5 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (2)

CD195 (CCR5) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 3

CD195 (CCR5) Antibody (46-1951-82) in Flow

Staining of FcR-blocked (Anti-Mouse CD16/CD32 Purified, Product # 14-0161-82) BALB/c peritoneal exudate cells with Anti-Mouse CD11b FITC (Product # 11-0112-41) and 0.125 µg of Armenian Hamster IgG Isotype Control PerCP-eFluor® 710 (Product # 46-4888-82) (left) or 0.125 µg of Anti-Mouse CD195 (CCR5) PerCP-eFluor® 710 (right). Cells in the large scatter population were used for analysis.

Product Image Gallery

Product Details

46-1951-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

View 5 publications 5 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), PerCP-eFluor™ 710, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

HM-CCR5 (7A4)

Conjugate

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

Excitation/Emission Max

482/708 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_11149119

Product Specific Information

Description: CCR5 is the major co-receptor for macrophage-tropic isolates of HIV-1. CCR5, also known as CD195, is a seven transmembrane chemokine receptor capable of binding RANTES, MIP-1 alpha and MIP-1 beta. Knockout mice show a decreased recruitment of macrophages and increased susceptibility to infection. Protein and mRNA has been found in macrophages from thioglycolate-treated and activated T cells.

The HM-CCR5(7A4) monoclonal antibody binds the N terminal extracellular domain of mouse CCR5 with no crossreactivity to human CCR5.

Applications Reported: This HM-CCR5 (7A4) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HM-CCR5 (7A4) antibody has been tested by flow cytometric analysis of mouse thioglycolated- induced peritoneal exudate cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.

Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Human immunodeficiency virus (HIV) and related virus require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors. Among them, CCR5 (CC-CKR-5) is a principal coreceptor for macrophage- and dual-tropic HIV-1 strains fusion and entry of human white blood cells. CCR5 is required for the infection by HIV-1, HIV-2, and SIV. The beta-chemokines RANTES, MIP-alpha and MIP-beta are the ligands for CCR5 and prevent infection by M-tropic HIV-1. CCR5 associates with the surface CD4-gp120 of HIV complex and leads to membrane fusion and virus entry of target cells. The amino-terminal domain and the extracellular loops of CCR5 serve as HIV biding sites. CCR5 messenger RNA is expressed in lymphoid organs and monocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

BCL-xL inhibition potentiates cancer therapies by redirecting the outcome of p53 activation from senescence to apoptosis.

Cell reports

Bharti V,Watkins R,Kumar A,Shattuck-Brandt RL,Mossing A,Mittra A,Shen C,Tsung A,Davies AE,Hanel W,Reneau JC,Chung C,Sizemore GM,Richmond A,Weiss VL,Vilgelm AE

46-1951-82 was used in Flow Cytometry to show a crosstalk between the mitochondrial apoptotic pathway and cell cycle regulation that can be targeted to augment therapeutic efficacy in cancers with wild-type p53.

Tue Dec 20 00:00:00 UTC 2022

Role of nuclear localization in the regulation and function of T-bet and Eomes in exhausted CD8 T cells.

Cell reports

McLane LM,Ngiow SF,Chen Z,Attanasio J,Manne S,Ruthel G,Wu JE,Staupe RP,Xu W,Amaravadi RK,Xu X,Karakousis GC,Mitchell TC,Schuchter LM,Huang AC,Freedman BD,Betts MR,Wherry EJ

46-1951-82 was used in Flow cytometry-immunoflourescence to investigate if subcellular localization of T-bet and Eomes dictate their regulatory activity in exhausted T cells (TEXs).

Tue May 11 00:00:00 UTC 2021

Growth Factor Screening in Dystrophic Muscles Reveals PDGFB/PDGFRB-Mediated Migration of Interstitial Stem Cells.

International journal of molecular sciences

Camps J,Grosemans H,Gijsbers R,Maes C,Sampaolesi M

Published figure using CD195 (CCR5) monoclonal antibody (Product # 46-1951-82) in Flow Cytometry

Tue Mar 05 00:00:00 UTC 2019

CD4⁺ T cells are activated in regional lymph nodes and migrate to skin to initiate lymphedema.

Nature communications

García Nores GD,Ly CL,Cuzzone DA,Kataru RP,Hespe GE,Torrisi JS,Huang JJ,Gardenier JC,Savetsky IL,Nitti MD,Yu JZ,Rehal S,Mehrara BJ

Published figure using CD195 (CCR5) monoclonal antibody (Product # 46-1951-82) in Flow Cytometry

Thu May 17 00:00:00 UTC 2018

Mitogen-Activated Protein Kinase 2 Signaling Shapes Macrophage Plasticity in Aggregatibacter actinomycetemcomitans-Induced Bone Loss.

Infection and immunity

Herbert BA,Steinkamp HM,Gaestel M,Kirkwood KL

46-1951 was used in Flow cytometry/Cell sorting to suggest that MK2 in macrophages contributes to regulation of chemokine signaling during A. actinomycetemcomitans-induced inflammation and bone loss.

Sun Jan 01 00:00:00 UTC 2017

Bioinformatics

Protein Aliases: C-C chemokine receptor type 5; C-C CKR-5; CC-CKR-5; CCR-5; CD195; CD195 antigen; chemokine (C-C) receptor 5; MIP-1 alpha receptor

View more View less

Gene Aliases: AM4-7; Ccr5; CD195; Cmkbr5

View more View less

UniProt ID: (Mouse) P51682

View more View less

Entrez Gene ID: (Mouse) 12774

View more View less

Function(s)

glycoprotein binding actin binding signal transducer activity G-protein coupled receptor activity chemokine receptor activity C-C chemokine receptor activity protein kinase binding C-C chemokine binding chemokine (C-C motif) ligand 5 binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD195 (CCR5) Monoclonal Antibody (HM-CCR5 (7A4)), PerCP-eFluor™ 710, eBioscience™

CD195 (CCR5) Antibody (46-1951-82) in Flow

Product Details

46-1951-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!