Invitrogen

CD195 (CCR5) Monoclonal Antibody (NP-6G4), PE, eBioscience™

2 Published Figures

7 References

View all (44) CCR5 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (2)

CD195 (CCR5) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 3

CD195 (CCR5) Antibody (12-1956-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD3 APC (Product # 17-0037-42) and 0.125 µg of Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or 0.125 µg of Anti-Human CD195 (CCR5) PE (right). Staining was performed at 37°C for hour and cells in the lymphocyte gate were used for analysis.

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Product Details

12-1956-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 7 publications 7 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

NP-6G4

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2572593

Product Specific Information

Description: The NP-6G4 monoclonal antibody reacts with human CD195 (CCR5), an approximately 45 kDa member of the G protein-coupled receptor (GPCR) superfamily that binds chemokines including MIP-1a, MIP-1b and RANTES. Human CCR5 is expressed on resting T cells with a memory/effector phenotype, monocytes, macrophages and immature dendritic cells. CCR5 functions as a receptor to direct the chemotaxis of lymphocytes, and is the primary co-receptor for macrophage-tropic Human Immunodeficiency Virus Type 1 (HIV-1), which binds CCR5 through gp120.

Applications Reported: This NP-6G4 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This NP-6G4 antibody has been pre-titrated and tested on normal human peripheral blood cells. For optimal performance, it is recommended that staining with NP-6G4 be done at 37°C for 1 hour in Flow Cytometry Staining Buffer (Product # 00-4222-57). This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Human immunodeficiency virus (HIV) and related virus require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors. Among them, CCR5 (CC-CKR-5) is a principal coreceptor for macrophage- and dual-tropic HIV-1 strains fusion and entry of human white blood cells. CCR5 is required for the infection by HIV-1, HIV-2, and SIV. The beta-chemokines RANTES, MIP-alpha and MIP-beta are the ligands for CCR5 and prevent infection by M-tropic HIV-1. CCR5 associates with the surface CD4-gp120 of HIV complex and leads to membrane fusion and virus entry of target cells. The amino-terminal domain and the extracellular loops of CCR5 serve as HIV biding sites. CCR5 messenger RNA is expressed in lymphoid organs and monocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Recombinant human IL-37 inhibited endometriosis development in a mouse model through increasing Th1/Th2 ratio by inducing the maturation of dendritic cells.

Reproductive biology and endocrinology : RB&E

Li L,Liao Z,Ye M,Jiang J

12-1956-42 was used in Flow cytometry/Cell sorting to study recombinant human IL-37 (rhIL-37) is an inhibitor of inflammation.

Tue Aug 24 00:00:00 UTC 2021

Application of the chemokine-chemokine receptor axis increases the tumor-targeted migration ability of cytokine-induced killer cells in patients with colorectal cancer.

Oncology letters

Zou Y,Liang J,Li D,Fang J,Wang L,Wang J,Zhang J,Guo Q,Yan X,Tang H

12-1956 was used in Flow cytometry/Cell sorting to demonstrate that modification of the CK-CKR axis using exogenous recombinant chemokines at the proper time point enhanced CIK cell trafficking ability and improved CIK antitumor effects.

Wed Jul 01 00:00:00 UTC 2020

Application of the chemokine-chemokine receptor axis increases the tumor-targeted migration ability of cytokine-induced killer cells in patients with colorectal cancer.

Oncology letters

Zou Y,Liang J,Li D,Fang J,Wang L,Wang J,Zhang J,Guo Q,Yan X,Tang H

Wed Jul 01 00:00:00 UTC 2020

T-bet+ Memory B Cells Link to Local Cross-Reactive IgG upon Human Rhinovirus Infection.

Cell reports

Eccles JD,Turner RB,Kirk NA,Muehling LM,Borish L,Steinke JW,Payne SC,Wright PW,Thacker D,Lahtinen SJ,Lehtinen MJ,Heymann PW,Woodfolk JA

12-1956-42 was used in Flow Cytometry to identify T-bet+ B cells binding both viruses that account for ∼5% of CXCR5- memory B cells.

Tue Jan 14 00:00:00 UTC 2020

Identification of Functionally Distinct Mx1+αSMA+ Periosteal Skeletal Stem Cells.

Cell stem cell

Ortinau LC,Wang H,Lei K,Deveza L,Jeong Y,Hara Y,Grafe I,Rosenfeld SB,Lee D,Lee B,Scadden DT,Park D

12-1956 was used in Flow cytometry/Cell sorting to report animal models that selectively and durably label postnatal Mx1+αSMA+ periosteal stem cells (P-SSCs) and establish that P-SSCs are a long-term repopulating, functionally distinct SSC subset responsible for lifelong generation of periosteal osteoblasts.

Thu Dec 05 00:00:00 UTC 2019

Early Pregnancy Human Decidua is Enriched with Activated, Fully Differentiated and Pro-Inflammatory Gamma/Delta T Cells with Diverse TCR Repertoires.

International journal of molecular sciences

Terzieva A,Dimitrova V,Djerov L,Dimitrova P,Zapryanova S,Hristova I,Vangelov I,Dimova T

12-1956-42 was used in Flow Cytometry to investigate the proportion phenotype and TCR γ and δ repertoires of γδ T cells at the maternal?fetal interface and in the blood of pregnant women.

Tue Feb 05 00:00:00 UTC 2019

Vitamin D regulates cytokine patterns secreted by dendritic cells to promote differentiation of IL-22-producing T cells.

PloS one

Sommer A,Fabri M

12-1956 was used in Flow cytometry/Cell sorting to study the role of vitamin D in regulating dendritic cell-mediated immune responses in humans.

Tue Apr 26 00:00:00 UTC 2016

Failure to upregulate cell surface PD-1 is associated with dysregulated stimulation of T cells by TGN1412-like CD28 superagonist.

mAbs

Thaventhiran T,Alhumeed N,Yeang HX,Sethu S,Downey JS,Alghanem AF,Olayanju A,Smith EL,Cross MJ,Webb SD,Williams DP,Bristow A,Ball C,Stebbings R,Sathish JG

12-1956 was used in Flow cytometry/Cell sorting to understand the underlying molecular mechanisms of T-cell activation in response to TGN1412-like CD28 superagonist, showing that failure to upregulate the inhibitory PD-1 cell surface receptor is part of this mechanism.

Mon Aug 24 00:00:00 UTC 2015

Failure to upregulate cell surface PD-1 is associated with dysregulated stimulation of T cells by TGN1412-like CD28 superagonist.

mAbs

Thaventhiran T,Alhumeed N,Yeang HX,Sethu S,Downey JS,Alghanem AF,Olayanju A,Smith EL,Cross MJ,Webb SD,Williams DP,Bristow A,Ball C,Stebbings R,Sathish JG

Mon Aug 24 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: C-C chemokine receptor type 5; C-C CKR-5; C-C motif chemokine receptor 5 A159A; CD195; CD195 antigen; chemokine (C-C motif) receptor 5; chemokine receptor CCR5; CHEMR13; HIV-1 fusion coreceptor

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Gene Aliases: CC-CKR-5; CCCKR5; CCR-5; CCR5; CD195; CKR-5; CKR5; CMKBR5; IDDM22

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UniProt ID: (Human) P51681

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Entrez Gene ID: (Human) 1234

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Function(s)

virus receptor activity actin binding phosphatidylinositol phospholipase C activity chemokine receptor activity protein binding coreceptor activity C-C chemokine receptor activity C-C chemokine binding chemokine (C-C motif) ligand 5 binding

Process(es)

MAPK cascade dendritic cell chemotaxis calcium ion transport chemotaxis inflammatory response immune response cellular defense response cell surface receptor signaling pathway G-protein coupled receptor signaling pathway positive regulation of cytosolic calcium ion concentration cell-cell signaling release of sequestered calcium ion into cytosol by sarcoplasmic reticulum fusion of virus membrane with host plasma membrane calcium-mediated signaling signaling entry into host cell chemokine-mediated signaling pathway response to cholesterol cellular response to lipopolysaccharide

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CD195 (CCR5) Monoclonal Antibody (NP-6G4), PE, eBioscience™

CD195 (CCR5) Antibody (12-1956-42) in Flow

Product Details

12-1956-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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