Invitrogen

CD195 (CCR5) Monoclonal Antibody (eBioT21/8 (T21/8)), eBioscience™

CD195 (CCR5) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 1

CD195 (CCR5) Antibody (14-1957-82) in Flow

Staining of normal human peripheral blood cells with 0.25 µg of Mouse IgG1 kappa Isotype Control Purified (Product # 14-4714-82) (open histogram) or 0.25 µg of Anti-Human CD195 (CCR5) Purified (filled histogram) followed by Anti-Mouse IgG Biotin (Product # 13-4013-85) and Streptavidin PE (Product # 12-4317-87). Cells in the lymphocyte gate were used for analysis.

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Product Details

14-1957-82

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-Dependent

View 1 publication 1 publication

Flow Cytometry (Flow)

0.5 µg/test

View 3 publications 3 publications

ELISA (ELISA)

Assay-Dependent

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-Dependent

Product Specifications

Species Reactivity

Human

Published species

Hamster, Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

eBioT21/8 (T21/8)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_657675

Product Specific Information

Description: The eBioT21/8 monoclonal antibody reacts with the amino terminus of human CCR5 (CD195), an approximately 45 kDa member of the G protein-coupled receptor (GPCR) superfamily that binds chemokines including MIP-1alpha, MIP-1beta and RANTES. Human CCR5 is expressed on resting T cells with a memory/effector phenotype, monocytes, macrophages and immature dendritic cells. CCR5 functions as a receptor to direct the chemotaxis of lymphocytes, and is the primary co-receptor for macrophage-tropic Human Immunodeficiency Virus Type 1 (HIV-1), which binds CCR5 through gp120. Binding of the eBioT21/8 monoclonal antibody displays donor variability and may be affected by sub-optimal staining conditions.

Applications Reported: This eBioT21/8 (T21/8) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB) and immunocytochemical staining. The eBioT21/8 monoclonal antibody has also been used as the capture antibody for the ELISA detection of human CCR5 in cell lysates.

Applications Tested: This eBioT21/8 (T21/8) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. For optimal performance, it is recommended that staining with NP-6G4 be done at 37°C for 1 hour in Flow Cytometry Staining Buffer (Product # 00-4222-57). This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Binding of the eBioT21/8 monoclonal antibody displays donor variability and may be affected by sup-optimal staining conditions. Therefore, staining conditions should be closely monitored to ensure consistent staining.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Human immunodeficiency virus (HIV) and related virus require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors. Among them, CCR5 (CC-CKR-5) is a principal coreceptor for macrophage- and dual-tropic HIV-1 strains fusion and entry of human white blood cells. CCR5 is required for the infection by HIV-1, HIV-2, and SIV. The beta-chemokines RANTES, MIP-alpha and MIP-beta are the ligands for CCR5 and prevent infection by M-tropic HIV-1. CCR5 associates with the surface CD4-gp120 of HIV complex and leads to membrane fusion and virus entry of target cells. The amino-terminal domain and the extracellular loops of CCR5 serve as HIV biding sites. CCR5 messenger RNA is expressed in lymphoid organs and monocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Immuno-oncological role of 20S proteasome alpha-subunit 3 in aggravating the progression of esophageal squamous cell carcinoma.

European journal of immunology

Liu J,Shao J,Zhang C,Qin G,Liu J,Li M,Wu P,Zhao X,Zhang Y

14-1957-82 was used in Flow cytometry/Cell sorting to demonstrate the role of PSMA3 in ESCC cancer stemness and the negative regulation of CD8 T-cells infiltration mediated by PSMA3 and provide a potential target for the immuno-oncology effect of PSMA3 in ESCC therapy.

Tue Feb 01 00:00:00 UTC 2022

Pericytes augment glioblastoma cell resistance to temozolomide through CCL5-CCR5 paracrine signaling.

Cell research

Zhang XN,Yang KD,Chen C,He ZC,Wang QH,Feng H,Lv SQ,Wang Y,Mao M,Liu Q,Tan YY,Wang WY,Li TR,Che LR,Qin ZY,Wu LX,Luo M,Luo CH,Liu YQ,Yin W,Wang C,Guo HT,Li QR,Wang B,Chen W,Wang S,Shi Y,Bian XW,Ping YF

14-1957-82 was used in Immunocytochemistry to reveal the role of pericytes as an extrinsic stimulator potentiating DDR signaling in GBM cells.

Fri Oct 01 00:00:00 UTC 2021

CCR5 susceptibility to ligand-mediated down-modulation differs between human T lymphocytes and myeloid cells.

Journal of leukocyte biology

Fox JM,Kasprowicz R,Hartley O,Signoret N

14-1957 was used in Flow cytometry/Cell sorting to compare the behavior of CCR5 on in vitro generated human T-cell blasts, monocytes and MDMs and CHO-CCR5 transfectants.

Wed Jul 01 00:00:00 UTC 2015

CCR5 susceptibility to ligand-mediated down-modulation differs between human T lymphocytes and myeloid cells.

Journal of leukocyte biology

Fox JM,Kasprowicz R,Hartley O,Signoret N

14-1957 was used in Flow cytometry/Cell sorting to compare the behavior of CCR5 on in vitro generated human T-cell blasts, monocytes and MDMs and CHO-CCR5 transfectants.

Wed Jul 01 00:00:00 UTC 2015

Prevailing role of contact guidance in intrastromal T-cell trapping in human pancreatic cancer.

Clinical cancer research : an official journal of the American Association for Cancer Research

Hartmann N,Giese NA,Giese T,Poschke I,Offringa R,Werner J,Ryschich E

14-1957 was used in Immunohistochemistry to analyse how chemokines and extracellular matrix collagen interact in mediating T-cell infiltration in pancreatic ductal adenocarcinoma.

Tue Jul 01 00:00:00 UTC 2014

Antibody epitopes on g protein-coupled receptors mapped with genetically encoded photoactivatable cross-linkers.

Biochemistry

Ray-Saha S,Huber T,Sakmar TP

14-1957 was used in an ELISA assay to develop a targeted photo-cross-linking strategy for mapping monoclonal antibody epitopes on G protein-coupled receptors.

Tue Mar 04 00:00:00 UTC 2014

Natural killer cells generated from cord blood hematopoietic progenitor cells efficiently target bone marrow-residing human leukemia cells in NOD/SCID/IL2Rg(null) mice.

PloS one

Cany J,van der Waart AB,Tordoir M,Franssen GM,Hangalapura BN,de Vries J,Boerman O,Schaap N,van der Voort R,Spanholtz J,Dolstra H

14-1957 was used in Flow cytometry/Cell sorting to support the therapeutic potential of ex vivo-generated UCB-NK cells in the treatment of myeloid leukemia after immunosuppressive chemotherapy.

Tue Jan 14 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: C-C chemokine receptor type 5; C-C CKR-5; C-C motif chemokine receptor 5 A159A; CD195; CD195 antigen; chemokine (C-C motif) receptor 5; chemokine receptor CCR5; CHEMR13; HIV-1 fusion coreceptor

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Gene Aliases: CC-CKR-5; CCCKR5; CCR-5; CCR5; CD195; CKR-5; CKR5; CMKBR5; IDDM22

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UniProt ID: (Human) P51681

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Entrez Gene ID: (Human) 1234

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Function(s)

virus receptor activity actin binding phosphatidylinositol phospholipase C activity chemokine receptor activity protein binding coreceptor activity C-C chemokine receptor activity C-C chemokine binding chemokine (C-C motif) ligand 5 binding

Process(es)

MAPK cascade dendritic cell chemotaxis calcium ion transport chemotaxis inflammatory response immune response cellular defense response cell surface receptor signaling pathway G-protein coupled receptor signaling pathway positive regulation of cytosolic calcium ion concentration cell-cell signaling release of sequestered calcium ion into cytosol by sarcoplasmic reticulum fusion of virus membrane with host plasma membrane calcium-mediated signaling signaling entry into host cell chemokine-mediated signaling pathway response to cholesterol cellular response to lipopolysaccharide

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD195 (CCR5) Monoclonal Antibody (eBioT21/8 (T21/8)), eBioscience™

CD195 (CCR5) Antibody (14-1957-82) in Flow

Product Details

14-1957-82

Western Blot (WB)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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