Invitrogen

CD196 (CCR6) Monoclonal Antibody (R6H1), PE, eBioscience™

1 Published Figure

6 References

View all (27) CCR6 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (1)

CD196 (CCR6) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 2

CD196 (CCR6) Antibody (12-1969-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD19 APC (Product # 17-0199-42) and Mouse IgG1 kappa Isotype Control PE (Product # 12-4714-81) (left) or Anti-Human CD196 (CCR6) PE (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

12-1969-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.06 µg)/test

View 6 publications 6 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

R6H1

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_1272087

Product Specific Information

Description: This R6H1 monoclonal antibody reacts with CD196 (also known as CCR6), a seven transmembrane G protein-coupled receptor expressed on T, B, dendritic, natural killer, and Langerhans cells. This CC chemokine receptor uniquely binds MIP-3a/CCL20, a chemoattractant for dendritic cells, effector/memory T cells, and B cells. CD196 is also involved in host defense and inflammation at epithelial sites. Furthermore, this receptor has been implicated in Th17 differentiation and CD4+FoxP3+ regulatory T cell development.

Applications Reported: This R6H1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This R6H1 antibody has been pre-titrated and tested by flow cytometric analysis on normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CCR6 is a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. The gene is preferentially expressed by immature dendritic cells and memory T cells. The ligand of this receptor is macrophage inflammatory protein 3 alpha (MIP-3 alpha). This receptor has been shown to be important for B-lineage maturation and antigen-driven B-cell differentiation, and it may regulate the migration and recruitment of dentritic and T cells during inflammatory and immunological responses. Alternatively spliced transcript variants that encode the same protein have been described for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

CD39⁺ regulatory T cells accumulate in colon adenocarcinomas and display markers of increased suppressive function.

Oncotarget

Ahlmanner F,Sundström P,Akeus P,Eklöf J,Börjesson L,Gustavsson B,Lindskog EB,Raghavan S,Quiding-Järbrink M

12-1969 was used in Flow cytometry/Cell sorting to treg therapy may hamper development of long-term tolerance and should be approached with caution in the clinical autologous setting.

Tue Dec 11 00:00:00 UTC 2018

Selective Expression of CCR10 and CXCR3 by Circulating Human Herpes Simplex Virus-Specific CD8 T Cells.

Journal of virology

Hensel MT,Peng T,Cheng A,De Rosa SC,Wald A,Laing KJ,Jing L,Dong L,Magaret AS,Koelle DM

12-1969 was used in Flow cytometry/Cell sorting to show that CCL27 drives baseline recruitment of HSV-specific CD8 T cells expressing CCR10.

Sun Oct 01 00:00:00 UTC 2017

Circulating and tumor-infiltrating mucosal associated invariant T (MAIT) cells in colorectal cancer patients.

Scientific reports

Ling L,Lin Y,Zheng W,Hong S,Tang X,Zhao P,Li M,Ni J,Li C,Wang L,Jiang Y

12-1969 was used in Flow cytometry/Cell sorting to examine the phenotype, distribution, clinical relevance, and potential function of mucosal associated invariant T cells in colorectal cancer patients.

Wed Feb 03 00:00:00 UTC 2016

Human Mucosa-Associated Invariant T Cells Accumulate in Colon Adenocarcinomas but Produce Reduced Amounts of IFN-γ.

Journal of immunology (Baltimore, Md. : 1950)

Sundström P,Ahlmanner F,Akéus P,Sundquist M,Alsén S,Yrlid U,Börjesson L,Sjöling Å,Gustavsson B,Wong SB,Quiding-Järbrink M

12-1969 was used in Flow cytometry/Cell sorting to elucidate the presence and function of mucosa-associated invariant T cells in tumours.

Thu Oct 01 00:00:00 UTC 2015

Secondary lymphoid organ homing phenotype of human myeloid dendritic cells disrupted by an intracellular oral pathogen.

Infection and immunity

Miles B,Zakhary I,El-Awady A,Scisci E,Carrion J,O'Neill JC,Rawlings A,Stern JK,Susin C,Cutler CW

12-1969 was used in Flow cytometry/Cell sorting to investigate how the alteration in chemokine-chemokine receptor expression contributes to pathogen trafficking via myeloid dendritic cells, showing that P.gingivalis infection induces an angiogenic migratory profile and disrupts secondary lymphoid organ-homing CCR7.

Wed Jan 01 00:00:00 UTC 2014

CCL20 Secretion from the Nucleus Pulposus Improves the Recruitment of CCR6-Expressing Th17 Cells to Degenerated IVD Tissues.

PloS one

Zhang W,Nie L,Wang Y,Wang XP,Zhao H,Dongol S,Maharjan S,Cheng L

Published figure using CD196 (CCR6) monoclonal antibody (Product # 12-1969-42) in Flow Cytometry

Fri Oct 13 00:00:00 UTC 2017

Bioinformatics

Protein Aliases: C-C chemokine receptor type 6; C-C CKR-6; CD196; chemokine (C-C motif) receptor 6; chemokine (C-C) receptor 6; Chemokine receptor-like 3; CKR-L3; CKR6; DRY-6; DRY6; G protein-coupled receptor 29; G-protein coupled receptor 29; GPR-CY4; GPRCY4; LARC receptor; seven-transmembrane receptor, lymphocyte, 22

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Gene Aliases: BN-1; C-C CKR-6; CC-CKR-6; CCR-6; CCR6; CD196; CKR-L3; CKRL3; CMKBR6; DCR2; DRY6; GPR29; GPRCY4; STRL22

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UniProt ID: (Human) P51684

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Entrez Gene ID: (Human) 1235

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Function(s)

receptor activity chemokine receptor activity protein binding C-C chemokine receptor activity C-C chemokine binding

Process(es)

dendritic cell chemotaxis leukocyte migration involved in inflammatory response movement of cell or subcellular component chemotaxis immune response humoral immune response cellular defense response signal transduction G-protein coupled receptor signaling pathway positive regulation of cytosolic calcium ion concentration positive regulation of epithelial cell migration calcium-mediated signaling isotype switching to IgA isotypes cell chemotaxis positive regulation of sperm motility involved in capacitation chemokine-mediated signaling pathway lymphocyte migration T cell migration thymocyte migration DN2 thymocyte differentiation DN3 thymocyte differentiation regulation of T cell migration positive regulation of dendritic cell chemotaxis

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CD196 (CCR6) Monoclonal Antibody (R6H1), PE, eBioscience™

CD196 (CCR6) Antibody (12-1969-42) in Flow

Product Details

12-1969-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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