Invitrogen

CD197 (CCR7) Monoclonal Antibody (4B12), Functional Grade, eBioscience™

16 Published Figures

43 References

View all (59) CCR7 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (16)

CD197 (CCR7) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 17

CD197 (CCR7) Antibody (16-1971-85) in Flow

RBL cells were transfected to co-express mouse CCR7 and GFP from an IRES-containing retrovirus.These cells were stained on ice for 2 hours with Rat IgG2a kappa Isotype Control PE (Product # 12-4321-80) (left) or 1 µg of Anti-Mouse CD197 (CCR7) Purified (right) followed by F (ab')2 Anti-Rat IgG PE. GFP expression is shown in FL1 on the X-axis. Total viable cells were used for analysis.

CD197 (CCR7) Antibody in Immunocytochemistry (ICC/IF)

Product Image Gallery

Product Details

16-1971-85

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

1 µg/test

View 37 publications 37 publications

Immunoprecipitation (IP)

Assay-Dependent

Neutralization (Neu)

View 1 publication 1 publication

Functional Assay (Functional)

Assay-Dependent

View 1 publication 1 publication

Control (Ctrl)

Assay-Dependent

Inhibition Assays (IA)

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Functional Grade, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

4B12

Conjugate

Functional Grade Functional Grade Functional Grade

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_494123

Product Specific Information

Description: The 4B12 monoclonal antibody reacts with mouse CCR7, also known as EBI-1 and CD197. CCR7 is a chemokine receptor for the chemokines CCL19 (CKβ11, ELC, MIP3β, Scya19, Exodus-3) and CCL21 (CKβ9, SLC, MIP2β, Scya21, Exodus-2). In recent years, the role of chemokines in directing the migration of lymphocytes has been well-characterized. One of the most important mediators of homeostatic trafficking of naive T cells to secondary lymphoid organs (SLO) is the chemokine receptor CCR7. Binding of its ligands, CCL19 and CCL21, mediates the trans endothelial migration of T cells across high endothelial venules into SLO. It has also been demonstrated that CCR7 plays a role in the localization of dendritic cells and B cells during an immune response.

CCR7 is a chemokine receptor for the chemokines CCL19 (CKβ11, ELC, MIP3β, Scya19, Exodus-3) and CCL21 (CKβ9, SLC, MIP2β, Scya21, Exodus-2).

In addition to its significant role in the chemotaxis of lymphocytes, human CCR7 has also been recognized as a marker for a distinct subset of memory T cells, the central memory (TCM) population. These cells are characterized by the expression of CCR7 and CD62L and reside within peripheral lymphoid organs. CCR7 also plays a role in thymocyte development and its deficiency leads to disturbed thymic architecture, aberrant T cell development, and limited thymocyte expansion.

For optimal visualization of CCR7 expression on different cell types it is necessary to use multi-color staining to discriminate different cell subsets as well as following the protocol (incubation at 37C may be necessary). To address specificity, the staining profile of 4B12 has been compared to a polyclonal antibody generated against a CCR7 peptide (Bjorkdahl et al). This analysis confirms that the polyclonal antibody and 4B12 stain similar populations of cells. Furthermore, 4B12 stains mouse CCR7-GFP fusion protein-transfected RBL cells (see data in Product # 14-1971).

Applications Reported: This 4B12 antibody has been reported for use in flow cytometric analysis, and immunoprecipitation.

Applications Tested: The 4B12 antibody has been tested by flow cytometric analysis of mouse splenocytes and thymocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Important: Staining with the 4B12 monoclonal antibody requires different conditions than typically used for surface-antigen staining. Please use the protocol below. Moreover, we have found that staining at 37°C, rather than 2-8°C, results in brighter 4B12 staining, as well as better resolution between positive and negative populations. Please see data for the PE 4B12 (Product # 12-1971) which demonstrates a comparison of staining at 2-8°C and 37°C. Staining with 4B12 at 37°C is not expected to interfere with co-staining other antigens, however this should be evaluated for individual experiments.

1. Prepare cell suspension as normal and block Fc gamma III R/Fc gamma II R with 5 µg/million cells purified anti-mouse CD16/32 (Product # 14-0161) for 15 minutes on ice. If red blood cell lysis is carried out as part of cell preparation, ensure that fixatives are not present in the red blood cell lysis solution as this will eliminate 4B12 staining.

2. Without washing, add 1 µg/million cells 4B12 and incubate in a 37°C waterbath or at 2-8°C (please see notes above) for 0.5 hours.

3. Wash cells 1X with 3 mL of Flow Cytometry Staining Buffer (Product # 00-4222) and decant supernatant.

4. Add secondary antibody and incubate at 2-8°C for 30 minutes.

5. Wash cells 1X with 3 mL of Flow Cytometry Staining Buffer (Product # 00-4222) and decant supernatant.Analyze cells on flow cytometer or proceed with secondary staining on ice as normal.

Note: Co-staining mouse CCR7 with the 4B12 antibody and the CCR7 ligand CCL19-Fc (Product # 14-1972) may be difficult due to different binding conditions required for the antibody versus the ligand, and steric hindrance which may prevent co-staining of 4B12 and CCL19-Fc. Cross-blocking experiments have demonstrated that 4B12 binding is able to prevent the detectable binding of CCL19-Fc, however not the opposite. Furthermore, the correlation between 4B12 and CCL19-Fc staining may be difficult to predict due to the presence of unknown CCL19-Fc receptors in addition to CCR7.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

CCR7 is a member of the G protein coupled receptor family, which is a subfamily of chemokines. CCR7 was identified to be induced by the Epstein Barr virus (EBV), and is thought to be a mediator of EBV effects on B lymphocytes. CCR7 has been reported to be expressed in blood, bone marrow, lymph node, and intestine. CCR7 is particularly expressed in lymphoid tissues and in activated B and T lymphocytes and has been shown to control the migration of memory T cells to inflamed tissues, as well as stimulate dendritic cell maturation. The chemokine (C-C motif) ligand 19 (CCL19/ECL) has been reported to be a specific ligand of this receptor. ESTs have been isolated from blood, embryo, lymph node, and thymus libraries. Receptors for the C - C chemokine family include CCR 1, CCR 2A, CCR 3, CCR 4, CCR 5 and the Duffy blood group antigen. The C-C receptors are important in the function of T cell chemotaxis and migration of phagocytic cells to sites of inflammation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Profiling of Tregs across tissues reveals plasticity in ST2 expression and hierarchies in tissue-specific phenotypes.

iScience

Spath S,Roan F,Presnell SR,Höllbacher B,Ziegler SF

Published figure using CD197 (CCR7) monoclonal antibody (Product # 16-1971-85) in Flow Cytometry

Fri Sep 16 00:00:00 UTC 2022

The allergy mediator histamine confers resistance to immunotherapy in cancer patients via activation of the macrophage histamine receptor H1.

Cancer cell

Li H,Xiao Y,Li Q,Yao J,Yuan X,Zhang Y,Yin X,Saito Y,Fan H,Li P,Kuo WL,Halpin A,Gibbons DL,Yagita H,Zhao Z,Pang D,Ren G,Yee C,Lee JJ,Yu D

16-1971-85 was used in Flow Cytometry to uncover that histamine and histamine receptor H1 (HRH1) are frequently increased in the tumor microenvironment and induce T cell dysfunction.

Mon Jan 10 00:00:00 UTC 2022

Mapping and role of T cell response in SARS-CoV-2-infected mice.

The Journal of experimental medicine

Zhuang Z,Lai X,Sun J,Chen Z,Zhang Z,Dai J,Liu D,Li Y,Li F,Wang Y,Zhu A,Wang J,Yang W,Huang J,Li X,Hu L,Wen L,Zhuo J,Zhang Y,Chen D,Li S,Huang S,Shi Y,Zheng K,Zhong N,Zhao J,Zhou D,Zhao J

Published figure using CD197 (CCR7) monoclonal antibody (Product # 16-1971-85) in Flow Cytometry

Mon Apr 05 00:00:00 UTC 2021

CD8⁺ T cells inhibit metastasis and CXCL4 regulates its function.

British journal of cancer

Joseph R,Soundararajan R,Vasaikar S,Yang F,Allton KL,Tian L,den Hollander P,Isgandarova S,Haemmerle M,Mino B,Zhou T,Shin C,Martinez-Paniagua M,Sahin AA,Rodriguez-Canales J,Gelovani J,Chang JT,Acharya G,Sood AK,Wistuba II,Gibbons DL,Solis LM,Barton MC,Varadarajan N,Rosen JM,Zhang XH,Mani SA

16-1971-85 was used in Mass cytometry to reveal that intratumoral immune infiltration differs between metastatic and non-metastatic tumours.

Thu Jul 01 00:00:00 UTC 2021

Carbomer-based adjuvant elicits CD8 T-cell immunity by inducing a distinct metabolic state in cross-presenting dendritic cells.

PLoS pathogens

Lee W,Kingstad-Bakke B,Paulson B,Larsen A,Overmyer K,Marinaik CB,Dulli K,Toy R,Vogel G,Mueller KP,Tweed K,Walsh AJ,Russell J,Saha K,Reyes L,Skala MC,Sauer JD,Shayakhmetov DM,Coon J,Roy K,Suresh M

Published figure using CD197 (CCR7) monoclonal antibody (Product # 16-1971-85) in Flow Cytometry

Fri Jan 01 00:00:00 UTC 2021

Prostaglandin E2 serves a dual role in regulating the migration of dendritic cells.

International journal of molecular medicine

Diao G,Huang J,Zheng X,Sun X,Tian M,Han J,Guo J

Published figure using CD197 (CCR7) monoclonal antibody (Product # 16-1971-85) in Flow Cytometry

Fri Jan 01 00:00:00 UTC 2021

A Versatile Toolkit for Semi-Automated Production of Fluorescent Chemokines to Study CCR7 Expression and Functions.

International journal of molecular sciences

Artinger M,Matti C,Gerken OJ,Veldkamp CT,Legler DF

Published figure using CD197 (CCR7) monoclonal antibody (Product # 16-1971-85) in Flow Cytometry

Fri Apr 16 00:00:00 UTC 2021

Anti-PD-1/L1 lead-in before MAPK inhibitor combination maximizes antitumor immunity and efficacy.

Cancer cell

Wang Y,Liu S,Yang Z,Algazi AP,Lomeli SH,Wang Y,Othus M,Hong A,Wang X,Randolph CE,Jones AM,Bosenberg MW,Byrum SD,Tackett AJ,Lopez H,Yates C,Solit DB,Ribas A,Piva M,Moriceau G,Lo RS

16-1971-85 was used in Flow Cytometry to demonstrate that anti-PD-1/L1 lead-in preceding MAPKi combination optimizes response durability by promoting pro-inflammatory polarization of macrophages and clonal expansion of interferon-γhi, and CD8+ cytotoxic and proliferative T cells.

Mon Oct 11 00:00:00 UTC 2021

E6‑regulated overproduction of prostaglandin E2 may inhibit migration of dendritic cells in human papillomavirus 16‑positive cervical lesions.

International journal of oncology

Huang J,Diao G,Zhang Q,Chen Y,Han J,Guo J

Published figure using CD197 (CCR7) monoclonal antibody (Product # 16-1971-85) in Flow Cytometry

Wed Apr 01 00:00:00 UTC 2020

Fluvastatin-Pretreated Donor Cells Attenuated Murine aGVHD by Balancing Effector T Cell Distribution and Function under the Regulation of KLF2.

BioMed research international

Zhao K,Tian Y,Wang J,Chen C,Pan B,Yan Z,Zhu S,Xu K

Published figure using CD197 (CCR7) monoclonal antibody (Product # 16-1971-85) in Flow Cytometry

Fri Sep 10 00:00:00 UTC 2021

Follicular Regulatory T Cells Can Access the Germinal Center Independently of CXCR5.

Cell reports

Vanderleyden I,Fra-Bido SC,Innocentin S,Stebegg M,Okkenhaug H,Evans-Bailey N,Pierson W,Denton AE,Linterman MA

Published figure using CD197 (CCR7) monoclonal antibody (Product # 16-1971-85) in Flow Cytometry

Tue Jan 21 00:00:00 UTC 2020

Inhibition of EZH2 ameliorates bacteria-induced liver injury by repressing RUNX1 in dendritic cells.

Cell death & disease

Wang Y,Wang Q,Wang B,Gu Y,Yu H,Yang W,Ren X,Qian F,Zhao X,Xiao Y,Zhang Y,Jin M,Zhu M

Published figure using CD197 (CCR7) monoclonal antibody (Product # 16-1971-85) in Flow Cytometry

Tue Dec 01 00:00:00 UTC 2020

MiR-103 protects from recurrent spontaneous abortion via inhibiting STAT1 mediated M1 macrophage polarization.

International journal of biological sciences

Zhu X,Liu H,Zhang Z,Wei R,Zhou X,Wang Z,Zhao L,Guo Q,Zhang Y,Chu C,Wang L,Li X

Published figure using CD197 (CCR7) monoclonal antibody (Product # 16-1971-85) in Flow Cytometry

Fri Nov 12 00:00:00 UTC 2021

Dendritic Cells and Myeloid Derived Suppressor Cells Fully Responsive to Stimulation via Toll-Like Receptor 4 Are Rapidly Induced from Bone-Marrow Cells by Granulocyte-Macrophage Colony-Stimulating Factor.

Vaccines

Kong YY,Wilson K,Apostolopoulos V,Plebanski M

Published figure using CD197 (CCR7) monoclonal antibody (Product # 16-1971-85) in Flow Cytometry

Sat Sep 12 00:00:00 UTC 2020

Sex Differences in Mouse Popliteal Lymph Nodes.

Scientific reports

Dill-Garlow R,Chen KE,Walker AM

Published figure using CD197 (CCR7) monoclonal antibody (Product # 16-1971-85) in Flow Cytometry

Wed Jan 30 00:00:00 UTC 2019

Aquaporin 4 inhibition alters chemokine receptor expression and T cell trafficking.

Scientific reports

Nicosia M,Miyairi S,Beavers A,Farr GW,McGuirk PR,Pelletier MF,Valujskikh A

Published figure using CD197 (CCR7) monoclonal antibody (Product # 16-1971-85) in Flow Cytometry

Wed May 15 00:00:00 UTC 2019

Induced migration of endothelial cells into 3D scaffolds by chemoattractants secreted by pro-inflammatory macrophages in situ.

Regenerative biomaterials

Li X,Dai Y,Shen T,Gao C

Published figure using CD197 (CCR7) monoclonal antibody (Product # 16-1971-85) in Immunofluorescence

Thu Jun 01 00:00:00 UTC 2017

Ginkgolide B Protects Against Ischemic Stroke Via Modulating Microglia Polarization in Mice.

CNS neuroscience & therapeutics

Shu ZM,Shu XD,Li HQ,Sun Y,Shan H,Sun XY,Du RH,Lu M,Xiao M,Ding JH,Hu G

Published figure using CD197 (CCR7) monoclonal antibody (Product # 16-1971-85) in Flow Cytometry

Thu Sep 01 00:00:00 UTC 2016

Thymic exosomes promote the final maturation of thymocytes.

Scientific reports

Lundberg V,Berglund M,Skogberg G,Lindgren S,Lundqvist C,Gudmundsdottir J,Thörn K,Telemo E,Ekwall O

Published figure using CD197 (CCR7) monoclonal antibody (Product # 16-1971-85) in Flow Cytometry

Tue Nov 08 00:00:00 UTC 2016

The E-Id protein axis modulates the activities of the PI3K-AKT-mTORC1-Hif1a and c-myc/p19Arf pathways to suppress innate variant TFH cell development, thymocyte expansion, and lymphomagenesis.

Genes & development

Miyazaki M,Miyazaki K,Chen S,Chandra V,Wagatsuma K,Agata Y,Rodewald HR,Saito R,Chang AN,Varki N,Kawamoto H,Murre C

16-1971 was used in Flow cytometry/Cell sorting to show that Id2 and Id3 suppress the development and expansion of innate variant follicular helper T cells.

Sun Feb 15 00:00:00 UTC 2015

Effector lymphocyte-induced lymph node-like vasculature enables naive T-cell entry into tumours and enhanced anti-tumour immunity.

Nature communications

Peske JD,Thompson ED,Gemta L,Baylis RA,Fu YX,Engelhard VH

16-1971 was used in Blocking experiments to establish LN-like vasculature as both a consequence of and key contributor to anti-tumour immunity.

Wed May 13 00:00:00 UTC 2015

Histone deacetylation critically determines T cell subset radiosensitivity.

Journal of immunology (Baltimore, Md. : 1950)

Pugh JL,Sukhina AS,Seed TM,Manley NR,Sempowski GD,van den Brink MR,Smithey MJ,Nikolich-Žugich J

16-1971 was used in Flow cytometry/Cell sorting to investigate the radiosensitivity of native, effector and central memory T cell subsets.

Fri Aug 01 00:00:00 UTC 2014

Temporal requirements for B cells in the establishment of CD4 T cell memory.

Journal of immunology (Baltimore, Md. : 1950)

Mollo SB,Zajac AJ,Harrington LE

16-1971 was used in Flow cytometry/Cell sorting to demonstrate the temporally and functionally distinct roles for B cells in regulating CD4 T cell responses.

Sun Dec 15 00:00:00 UTC 2013

Memory CD4(+)CCR5(+) T cells are abundantly present in the gut of newborn infants to facilitate mother-to-child transmission of HIV-1.

Blood

Bunders MJ,van der Loos CM,Klarenbeek PL,van Hamme JL,Boer K,Wilde JC,de Vries N,van Lier RA,Kootstra N,Pals ST,Kuijpers TW

16-1971 was used in Immunohistochemistry to investigate a large subset of mucosal memory CD4(+)CCR5(+) T cells with predominantly a Th1 and Th17 phenotype in the human foetal and infant gut mucosa.

Thu Nov 22 00:00:00 UTC 2012

Exposure to nicotine adversely affects the dendritic cell system and compromises host response to vaccination.

Journal of immunology (Baltimore, Md. : 1950)

Nouri-Shirazi M,Guinet E

16-1971 was used in Flow cytometry/Cell sorting to investigate the causal relationship between nicotine-induced dendritic cell alterations and host response to vaccines.

Thu Mar 01 00:00:00 UTC 2012

Optimal population of FoxP3+ T cells in tumors requires an antigen priming-dependent trafficking receptor switch.

PloS one

Wang C,Lee JH,Kim CH

16-1971 was used in Flow cytometry/Cell sorting to investigate the migration requirement and stability of tumour-associated FoxP3(+) T cells.

Mon Jun 04 00:00:00 UTC 2012

Sequential induction of effector function, tissue migration and cell death during polyclonal activation of mouse regulatory T-cells.

PloS one

Langenhorst D,Gogishvili T,Ribechini E,Kneitz S,McPherson K,Lutz MB,Hünig T

16-1971 was used in Flow cytometry/Cell sorting to investigate the origin and fate of IL-10 producing Tregs.

Mon May 20 00:00:00 UTC 2013

The Journal of clinical investigation

Zhao J,Zhao J,Legge K,Perlman S

16-1971 was used in Flow cytometry/Cell sorting to study the effect of increased PGD(2) expression on the viral immune response.

Thu Dec 01 00:00:00 UTC 2011

Retinoic acid as a vaccine adjuvant enhances CD8+ T cell response and mucosal protection from viral challenge.

Journal of virology

Tan X,Sande JL,Pufnock JS,Blattman JN,Greenberg PD

16-1971 was used in Flow cytometry/Cell sorting to test whether the administration of retinoic acid could establish better protection from viral infection via mucosal routes.

Mon Aug 01 00:00:00 UTC 2011

The phosphatase SRC homology region 2 domain-containing phosphatase-1 is an intrinsic central regulator of dendritic cell function.

Journal of immunology (Baltimore, Md. : 1950)

Ramachandran IR,Song W,Lapteva N,Seethammagari M,Slawin KM,Spencer DM,Levitt JM

16-1971-85 was used in Neutralization to demonstrate that SHP-1 is an intrinsic global regulator of dendritic cell function, controlling many facets of T cell-mediated immune responses.

Fri Apr 01 00:00:00 UTC 2011

Rescue of tumor-infiltrating lymphocytes from activation-induced cell death enhances the antitumor CTL response in CD5-deficient mice.

Journal of immunology (Baltimore, Md. : 1950)

Tabbekh M,Franciszkiewicz K,Haouas H,Lécluse Y,Benihoud K,Raman C,Mami-Chouaib F

16-1971 was used in Flow cytometry/Cell sorting to compare the T cell response to the B16F10 melanoma engrafted into CD5-deficient and wild-type C57BL/6 mice.

Fri Jul 01 00:00:00 UTC 2011

Novel CCL21-vault nanocapsule intratumoral delivery inhibits lung cancer growth.

PloS one

Kar UK,Srivastava MK,Andersson A,Baratelli F,Huang M,Kickhoefer VA,Dubinett SM,Rome LH,Sharma S

16-1971 was used in Western Blotting to demonstrate that the vault nanocapsule can efficiently deliver CCL21 to recruit antitumour effectors, sustain antitumour activity and inhibit tumour growth.

Tue May 03 00:00:00 UTC 2011

Emergence of anthrax edema toxin as a master manipulator of macrophage and B cell functions.

Toxins

Gnade BT,Moen ST,Chopra AK,Peterson JW,Yeager LA

16-1971 was used in Flow cytometry/Cell sorting to investigate the influence of anthrax edema toxin on macrophage and B-cell functions.

Thu Jul 01 00:00:00 UTC 2010

Professional memory CD4+ T lymphocytes preferentially reside and rest in the bone marrow.

Immunity

Tokoyoda K,Zehentmeier S,Hegazy AN,Albrecht I,Grün JR,Löhning M,Radbruch A

16-1971 was used in Flow cytometry/Cell sorting to show that in the memory phase, memory helper T cells are maintained in BM as resting but highly reactive cells in survival niches defined by IL-7-expressing stroma cells.

Fri May 01 00:00:00 UTC 2009

Lymphoid tissue inducer-like cells are an innate source of IL-17 and IL-22.

The Journal of experimental medicine

Takatori H,Kanno Y,Watford WT,Tato CM,Weiss G,Ivanov II,Littman DR,O'Shea JJ

16-1971 was used in Flow cytometry/Cell sorting to investigate immune cells as a source of interleukins for host defence.

Fri Jan 16 00:00:00 UTC 2009

Migratory and antigen presentation functions of IFN-producing killer dendritic cells.

Cancer research

Himoudi N,Yan M,Bouma G,Morgenstern D,Wallace R,Seddon B,Buddle J,Eddaoudi A,Howe SJ,Cooper N,Anderson J

16-1971 was used in Flow cytometry/Cell sorting to study how BM-IKDCs are capable of cytotoxic killing of tumour targets, and also of potent antigen presentation after encountering antigen in the context of a viable target cell.

Sat Aug 15 00:00:00 UTC 2009

Co-immunization with an optimized plasmid-encoded immune stimulatory interleukin, high-mobility group box 1 protein, results in enhanced interferon-gamma secretion by antigen-specific CD8 T cells.

Immunology

Muthumani G,Laddy DJ,Sundaram SG,Fagone P,Shedlock DJ,Kannan S,Wu L,Chung CW,Lankaraman KM,Burns J,Muthumani K,Weiner DB

Published figure using CD197 (CCR7) monoclonal antibody (Product # 16-1971-85) in Flow Cytometry

Tue Sep 01 00:00:00 UTC 2009

IFN-gamma promotes generation of IL-10 secreting CD4+ T cells that suppress generation of CD8 responses in an antigen-experienced host.

Journal of immunology (Baltimore, Md. : 1950)

Liu XS,Leerberg J,MacDonald K,Leggatt GR,Frazer IH

16-1971 was used in Western Blotting to study the mechanism by which CD8 T cell response is inhibited by CD4(+) T cells in antigen-experienced hosts, showing that IL-10 secretion by CD4(+) cells is enhanced by IFM-gamma signalling, which then inhibits CD8 generation, suggesting that inhibition of IL-10 and IFN-gamma could increase antitumor effect when used in conjunction with antigen immunotherapy.

Wed Jul 01 00:00:00 UTC 2009

CD40L+ CD4+ memory T cells migrate in a CD62P-dependent fashion into reactive lymph nodes and license dendritic cells for T cell priming.

The Journal of experimental medicine

Martín-Fontecha A,Baumjohann D,Guarda G,Reboldi A,Hons M,Lanzavecchia A,Sallusto F

16-1971 was used in Flow cytometry/Cell sorting to show that T(EM) cells can behave as endogenous adjuvants.

Mon Oct 27 00:00:00 UTC 2008

Selected natural and synthetic retinoids impair CCR7- and CXCR4-dependent cell migration in vitro and in vivo.

Journal of leukocyte biology

Villablanca EJ,Zhou D,Valentinis B,Negro A,Raccosta L,Mauri L,Prinetti A,Sonnino S,Bordignon C,Traversari C,Russo V

16-1971 was used in Flow cytometry/Cell sorting to study a novel function of retinoids in impairing CCR7- and CXCR4-dependent cell migration in vitro and in vivo.

Mon Sep 01 00:00:00 UTC 2008

CCR7 mediates the migration of Foxp3+ regulatory T cells to the paracortical areas of peripheral lymph nodes through high endothelial venules.

Journal of leukocyte biology

Ueha S,Yoneyama H,Hontsu S,Kurachi M,Kitabatake M,Abe J,Yoshie O,Shibayama S,Sugiyama T,Matsushima K

16-1971 was used in Flow cytometry/Cell sorting to provide the evidence that CCR7 mediates nTreg migration to the paracortical areas of lymph nodes under steady-state conditions, but also takes place in the medulla.

Thu Nov 01 00:00:00 UTC 2007

The autoimmune diabetes locus Idd9 regulates development of type 1 diabetes by affecting the homing of islet-specific T cells.

Journal of immunology (Baltimore, Md. : 1950)

Waldner H,Sobel RA,Price N,Kuchroo VK

Mon May 01 00:00:00 UTC 2006

Characterization of CC-chemokine receptor 7 expression on murine T cells in lymphoid tissues.

Immunology

Bjorkdahl O,Barber KA,Brett SJ,Daly MG,Plumpton C,Elshourbagy NA,Tite JP,Thomsen LL

Published figure using CD197 (CCR7) monoclonal antibody (Product # 16-1971-85) in Flow Cytometry

Wed Oct 01 00:00:00 UTC 2003

Bioinformatics

Protein Aliases: C-C chemokine receptor type 7; C-C CKR-7; CD197; chemokine (C-C) receptor 7; EBI1; EBV-induced G-protein coupled receptor 1; Epstein-Barr virus-induced G-protein coupled receptor 1; MIP-3 beta receptor

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Gene Aliases: CC-CKR-7; CCR-7; Ccr7; CD197; Cdw197; Cmkbr7; EBI1; Ebi1h

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UniProt ID: (Mouse) P47774

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Entrez Gene ID: (Mouse) 12775

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Function(s)

signal transducer activity G-protein coupled receptor activity chemokine receptor activity protein binding C-C chemokine receptor activity chemokine (C-C motif) ligand 19 binding chemokine (C-C motif) ligand 21 binding C-C motif chemokine 19 receptor activity C-C motif chemokine 21 receptor activity

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD197 (CCR7) Monoclonal Antibody (4B12), Functional Grade, eBioscience™

CD197 (CCR7) Antibody (16-1971-85) in Flow

Product Details

16-1971-85

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Neutralization (Neu)

Functional Assay (Functional)

Control (Ctrl)

Inhibition Assays (IA)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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