Invitrogen

CD1c Monoclonal Antibody (L161), APC, eBioscience™

5 Published Figures

8 References

View all (71) CD1c antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (5)

FIGURE: 1 / 6

CD1c Antibody (17-0015-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD19 eFluor® 450 (Product # 48-0199-42) and Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (left) or Anti-Human CD1c APC (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

17-0015-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.06 µg)/test

View 8 publications 8 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Rhesus monkey

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

L161

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10598518

Product Specific Information

Description: This L161 monoclonal antibody detects CD1c (also known as BDCA-1), a glycoprotein that is noncovalently linked to beta-2 microglobulin on thymocytes and antigen presenting cells such as dendritic and Langerhans cells. This molecule is also expressed on some circulating and marginal zone B cells, as well as in lymph nodes and germinal centers. CD1c is involved in the presentation of lipid antigens such as microbial fatty acids to effector T cells during the adaptive immune response. Finally, alternative splicing gives rise to three different isoforms of CD1c (soluble, membrane, and cytoplasmic/soluble isoforms).

Applications Reported: This L161 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This L161 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD1c is a member of the CD1 family of transmembrane glycoproteins, which are structurally related to major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. This family of proteins is involved in the presentation of lipid and glycolipid antigens, both of self and microbial origin, to T cells during the adaptive immune response. CD1c is expressed on some circulating and marginal zone B cells, as well as in lymph nodes and germinal centers. It plays a crucial role in presenting lipid antigens, such as microbial fatty acids, to effector T cells. The protein encoded by the CD1c gene localizes to late endosomes and lysosomes, utilizing a tyrosine-based motif in its cytoplasmic tail for targeting. Vesicular acidification is required for CD1c to bind lipid antigens effectively. The human genome contains five CD1 family genes organized in a cluster on chromosome 1, with each member differing in cellular localization and specificity for particular lipid ligands. CD1c undergoes alternative splicing, resulting in three different isoforms: soluble, membrane-bound, and cytoplasmic/soluble isoforms, highlighting its functional diversity in immune processes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Engineered Human Dendritic Cell Exosomes as Effective Delivery System for Immune Modulation.

International journal of molecular sciences

Elsayed R,Elashiry M,Tran C,Yang T,Carroll A,Liu Y,Hamrick M,Cutler CW

Published figure using CD1c monoclonal antibody (Product # 17-0015-42) in Flow Cytometry

Tue Jul 11 00:00:00 UTC 2023

Heme oxygenase-1 determines the cell fate of ferroptotic death of alveolar macrophages in COPD.

Frontiers in immunology

Li Y,Yang Y,Guo T,Weng C,Yang Y,Wang Z,Zhang L,Li W

17-0015-42 was used in Flow cytometry/Cell sorting to identify two populations of alveolar macrophages (AMs) in the human lung that were dysregulated in COPD patients.

Wed May 24 00:00:00 UTC 2023

Heme oxygenase-1 determines the cell fate of ferroptotic death of alveolar macrophages in COPD.

Frontiers in immunology

Li Y,Yang Y,Guo T,Weng C,Yang Y,Wang Z,Zhang L,Li W

17-0015-42 was used in Flow cytometry/Cell sorting to identify two populations of alveolar macrophages (AMs) in the human lung that were dysregulated in COPD patients.

Wed May 24 00:00:00 UTC 2023

Novel application of [¹⁸F]DPA714 for visualizing the pulmonary inflammation process of SARS-CoV-2-infection in rhesus monkeys (Macaca mulatta).

Nuclear medicine and biology

Meijer L,Böszörményi KP,Bakker J,Koopman G,Mooij P,Verel D,Fagrouch Z,Verstrepen BE,Funke U,Mooijer MPJ,Langermans JAM,Verschoor EJ,Windhorst AD,Stammes MA

17-0015-42 was used in Flow cytometry/Cell sorting to investigate the application of [18F]DPA714 to visualize the inflammation process in the lungs of SARS-CoV-2-infected rhesus monkeys, focusing on the presence of pulmonary lesions, activation of mediastinal lymph nodes and surrounded lung tissue.

Tue Oct 04 00:00:00 UTC 2022

DNAM1 and TIGIT balance the T cell response, with low T cell TIGIT expression corresponding to inflammation in psoriatic disease.

Immunotherapy advances

Jacobs ME,Pouw JN,Olde Nordkamp MA,Radstake TRDJ,Leijten EFA,Boes M

Published figure using CD1c monoclonal antibody (Product # 17-0015-42) in Flow Cytometry

Fri Jan 01 00:00:00 UTC 2021

Dendritic cells' characteristics in patients with treated systemic lupus erythematosus.

Acta biochimica Polonica

Wardowska A,Smoleńska Ż,Lisowska KA,Zdrojewski Z,Pikuła M

17-0015-42 was used in Flow Cytometry to study alterations in dendritic cells at the cellular and molecular levels in patients with treated systemic lupus erythematosus, depending on the disease activity and treatment.

Thu Jul 30 00:00:00 UTC 2020

STAT3 Inhibition Combined with CpG Immunostimulation Activates Antitumor Immunity to Eradicate Genetically Distinct Castration-Resistant Prostate Cancers.

Clinical cancer research : an official journal of the American Association for Cancer Research

Moreira D,Adamus T,Zhao X,Su YL,Zhang Z,White SV,Swiderski P,Lu X,DePinho RA,Pal SK,Kortylewski M

17-0015 was used in Flow cytometry/Cell sorting to show a novel strategy combining STAT3 inhibition with Toll-like Receptor 9 (TLR9) stimulation to unleash immune response against prostate cancers regardless of the genetic background.

Sat Dec 01 00:00:00 UTC 2018

Malaria-induced NLRP12/NLRP3-dependent caspase-1 activation mediates inflammation and hypersensitivity to bacterial superinfection.

PLoS pathogens

Ataide MA,Andrade WA,Zamboni DS,Wang D,Souza Mdo C,Franklin BS,Elian S,Martins FS,Pereira D,Reed G,Fitzgerald KA,Golenbock DT,Gazzinelli RT

Published figure using CD1c monoclonal antibody (Product # 17-0015-42) in Flow Cytometry

Wed Jan 01 00:00:00 UTC 2014

CD36 contributes to malaria parasite-induced pro-inflammatory cytokine production and NK and T cell activation by dendritic cells.

PloS one

Gowda NM,Wu X,Kumar S,Febbraio M,Gowda DC

17-0015 was used in Flow cytometry/Cell sorting to imply that the effect of CD36 on malaria immunity is imprinted early during infection when parasite load is low.

Tue Aug 05 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: CD1c; CD1C antigen, c polypeptide; cortical thymocyte antigen CD1C; differentiation antigen CD1-alpha-3; RP11-101J8.3; T-cell surface glycoprotein CD1c

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Gene Aliases: BDCA1; CD1; CD1A; CD1C; R7

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UniProt ID: (Human) P29017

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Entrez Gene ID: (Human) 911

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Function(s)

beta-2-microglobulin binding endogenous lipid antigen binding exogenous lipid antigen binding glycolipid binding lipopeptide binding

Process(es)

adaptive immune response T cell activation involved in immune response antigen processing and presentation, exogenous lipid antigen via MHC class Ib regulation of immune response

Performance Guarantee

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CD1c Monoclonal Antibody (L161), APC, eBioscience™

CD1c Antibody (17-0015-42) in Flow

Product Details

17-0015-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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