Invitrogen

CD1d Monoclonal Antibody (1B1), Alexa Fluor™ 488, eBioscience™

5 Published Figures

7 References

View all (45) CD1d antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (5)

FIGURE: 1 / 6

CD1d Antibody (53-0011-82) in Flow

Staining of C57BL/6 splenocytes with Anti-Human/Mouse CD45R (B220) APC (Product # 17-0452-82) and Rat IgG2b K Isotype Control Alexa Fluor® 488 (Product # 53-4031-80) (left) or 0.5 µg of Anti-Mouse CD1d Alexa Fluor® 488 (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

53-0011-82

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Flow Cytometry (Flow)

1 µg/test

View 6 publications 6 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), Alexa Fluor™ 488, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

1B1

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_1944357

Product Specific Information

Description: The 1B1 monoclonal antibody reacts with mouse CD1d, a 48 kDa glycoprotein with structural homology to MHC class I molecules. While similar to MHC Class I, CD1d associates with beta2m, functionally CD1d is similar to MHC Class II. 1B1 detects CD1d at varying levels on mouse leukocytes. 1B1 detects beta2m associated CD1d.

Applications Reported: This 1B1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 1B1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD1d is a heavy chain associated with Beta 2 microglobulin on cortical thymocytes. Beta 2 microglobulin independent expression of CD1d has also been demonstrated on human intestinal epithelial cells. CD1d is the sole group 2 member of the CD1 family of major histocompatibility (MHC) like glycoproteins. The CD1d gene encodes a divergent member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by the CD1d gene localizes to late endosomes and lysosomes via a tyrosine-based motif in the cytoplasmic tail. Diseases associated with CD1D include Mycobacterium Malmoense and Autoimmune Disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Methotrexate-loaded nanoparticles ameliorate experimental model of autoimmune arthritis by regulating the balance of interleukin-17-producing T cells and regulatory T cells.

Journal of translational medicine

Park JS,Lee D,Yang S,Jeong HY,Na HS,Cho KH,Choi J,Koo H,Cho ML,Park SH

Published figure using CD1d monoclonal antibody (Product # 53-0011-82) in Flow Cytometry

Fri Feb 11 00:00:00 UTC 2022

Tregitopes regulate the tolerogenic immune response and decrease the foetal death rate in abortion-prone mouse matings.

Scientific reports

Kedzierska AE,Lorek D,Slawek A,Chelmonska-Soyta A

Published figure using CD1d monoclonal antibody (Product # 53-0011-82) in Flow Cytometry

Mon Jun 29 00:00:00 UTC 2020

Loss of IP₃ Receptor-Mediated Ca²⁺ Release in Mouse B Cells Results in Abnormal B Cell Development and Function.

Journal of immunology (Baltimore, Md. : 1950)

Tang H,Wang H,Lin Q,Fan F,Zhang F,Peng X,Fang X,Liu J,Ouyang K

53-0011 was used in Flow cytometry/Cell sorting to determine the function of 1,4,5-trisphosphate receptor-mediated Ca2+ release in B cells.

Sat Jul 15 00:00:00 UTC 2017

Multiple functional therapeutic effects of TnP: A small stable synthetic peptide derived from fish venom in a mouse model of multiple sclerosis.

PloS one

Komegae EN,Souza TA,Grund LZ,Lima C,Lopes-Ferreira M

Published figure using CD1d monoclonal antibody (Product # 53-0011-82) in Flow Cytometry

Thu Aug 17 00:00:00 UTC 2017

NFATc1 supports imiquimod-induced skin inflammation by suppressing IL-10 synthesis in B cells.

Nature communications

Alrefai H,Muhammad K,Rudolf R,Pham DA,Klein-Hessling S,Patra AK,Avots A,Bukur V,Sahin U,Tenzer S,Goebeler M,Kerstan A,Serfling E

Published figure using CD1d monoclonal antibody (Product # 53-0011-82) in Flow Cytometry

Wed May 25 00:00:00 UTC 2016

FHL1 reduces dystrophy in transgenic mice overexpressing FSHD muscular dystrophy region gene 1 (FRG1).

PloS one

Feeney SJ,McGrath MJ,Sriratana A,Gehrig SM,Lynch GS,D'Arcy CE,Price JT,McLean CA,Tupler R,Mitchell CA

Published figure using CD1d monoclonal antibody (Product # 53-0011-82) in Western Blot

Mon Nov 16 00:00:00 UTC 2015

Nuclear export of the NF-κB inhibitor IκBα is required for proper B cell and secondary lymphoid tissue formation.

Immunity

Wuerzberger-Davis SM,Chen Y,Yang DT,Kearns JD,Bates PW,Lynch C,Ladell NC,Yu M,Podd A,Zeng H,Huang TT,Wen R,Hoffmann A,Wang D,Miyamoto S

Published figure using CD1d monoclonal antibody (Product # 53-0011-82) in Flow Cytometry

Fri Feb 25 00:00:00 UTC 2011

Bioinformatics

Protein Aliases: Antigen-presenting glycoprotein CD1d1; CD1d.1; T-cell surface glycoprotein CD1d1

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Gene Aliases: AI747460; CD1.1; Cd1a; Cd1d; Cd1d1; Ly-38

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UniProt ID: (Mouse) P11609

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Entrez Gene ID: (Mouse) 12479

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Function(s)

protein binding beta-2-microglobulin binding lipid antigen binding endogenous lipid antigen binding exogenous lipid antigen binding histone binding T cell receptor binding cell adhesion molecule binding lipopeptide binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD1d Monoclonal Antibody (1B1), Alexa Fluor™ 488, eBioscience™

CD1d Antibody (53-0011-82) in Flow

Product Details

53-0011-82

Western Blot (WB)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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