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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • CD20 Antibodies

          Invitrogen

          CD20 Polyclonal Antibody

          Advanced Verification
          26 Published Figures
          74 References
          View all (223) CD20 antibodies

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          Datasheet
          Protocols
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          Datasheet
          Protocols
          Questions & Answers

          Cite CD20 Polyclonal Antibody

          • Antibody Testing Data (8)
          • Published Figures (26)
          • Advanced Verification (2)
          CD20 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          CD20 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 36

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          CD20 Antibody (PA5-16701) in ICC/IF

          Immunofluorescence analysis of CD20 was performed using 70% confluent log phase Ramos cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CD20 Polyclonal Antibody (Product # PA5-16701) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790) at a dilu... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          CD20 Antibody in Immunocytochemistry (ICC/IF)
          CD20 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CD20 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CD20 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CD20 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CD20 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CD20 Antibody in Western Blot (WB)
          CD20 Antibody in Western Blot (WB)
          CD20 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CD20 Antibody in Immunohistochemistry (IHC)
          CD20 Antibody in Immunohistochemistry (IHC)
          CD20 Antibody in Immunocytochemistry (ICC/IF)
          CD20 Antibody in Immunohistochemistry (IHC)
          CD20 Antibody in Immunohistochemistry (IHC)
          CD20 Antibody in Immunohistochemistry (IHC)
          CD20 Antibody in Immunohistochemistry (IHC)
          CD20 Antibody in Immunohistochemistry (IHC)
          CD20 Antibody in Immunohistochemistry (IHC)
          CD20 Antibody in Immunohistochemistry (IHC)
          CD20 Antibody in Immunohistochemistry (IHC)
          CD20 Antibody in Immunohistochemistry (IHC)
          CD20 Antibody in Immunohistochemistry (IHC)
          CD20 Antibody in Immunohistochemistry (IHC)
          CD20 Antibody in Immunohistochemistry (IHC)
          CD20 Antibody in Immunohistochemistry (IHC)
          CD20 Antibody in Immunohistochemistry (IHC)
          CD20 Antibody in Immunohistochemistry (IHC)
          CD20 Antibody in Immunohistochemistry (IHC)
          CD20 Antibody in Immunohistochemistry (IHC)
          CD20 Antibody in Immunohistochemistry (IHC)
          CD20 Antibody in Immunohistochemistry (IHC)
          CD20 Antibody in Immunohistochemistry (IHC)
          CD20 Antibody in Western Blot (WB)
          CD20 Antibody in Western Blot (WB)
          CD20 Antibody
          CD20 Antibody

          Product Details

          PA5-16701

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:2,000
          View 2 publications 2 publications

          Immunohistochemistry (IHC)

          -
          View 54 publications 54 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:300
          View 10 publications 10 publications

          Immunohistochemistry (Frozen) (IHC (F))

          -
          View 2 publications 2 publications

          Immunocytochemistry (ICC/IF)

          1:100
          View 5 publications 5 publications

          Miscellaneous PubMed (MISC)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Dog, Cat, Human, Mouse

          Published species

          Bovine, Cat, Dog, Horse, Human, Mink, Mouse, Mustelid, Non-human primate, Pig, Rat, Rhesus monkey, Sheep

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Synthetic peptide derived from C-terminus of human CD20 protein
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.08 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.6, with 1% BSA

          Contains

          0.1% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          RRID

          AB_10980806

          Product Specific Information

          Upon delivery aliquot and store at -20ºC, avoiding freeze/thaw cycles.

          For IHC staining, antigen retrieval is not essential but may optimize staining. Recommended positive controls: BJAB WCL and tonsil.

          Target Information

          CD20 is a non-glycosylated surface phosphoprotein that has a molecular weight range of 33-37 kDa depending on the degree of phosphorylation. CD20 is expressed on mature and most malignant B cells, in a subpopulation of T lymphocytes and follicular dendritic cells. CD20 expression on B cells is synchronous with the expression of surface IgM and it regulates transmembrane calcium conductance, cell cycle progression and B-cell proliferation. CD20 is also associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change, and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following monoclonal antibody treatment. Diseases associated with CD20 dysfunction include Ms4a1-related common variable immune deficiency.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: APY; ATOPY; B-cell differentiation antigen Ly-44; B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; B-lymphocyte surface antigen B1; Bp35; CD20; CD20 antigen; CD20 cell surface protein; CD20 receptor; Fc epsilon receptor I beta chain; Fc Fragment of IgE high affinity I receptor for beta polypeptide; FCER1B; High affinity immunoglobulin epsilon receptor subunit beta; IgE Fc receptor subunit beta; IGEL; IGER; IGHER; LEU16; Leukocyte surface antigen Leu-16; Ly44; Lymphocyte antigen 44; Membrane-spanning 4-domains subfamily A member 1; membrane-spanning 4-domains, subfamily A, member 1; membrane-spanning 4-domains, subfamily A, member 2; MGC3969

          View more View less

          Gene Aliases: AA960661; B1; Bp35; CD20; CVID5; LEU-16; Ly-44; MS4A1; MS4A2; S7

          View more View less

          UniProt ID: (Human) P11836, (Dog) Q3C2E2, (Mouse) P19437

          View more View less

          Entrez Gene ID: (Human) 931, (Cat) 494216, (Dog) 485430, (Mouse) 12482

          View more View less

          Function(s)
          epidermal growth factor receptor binding protein binding MHC class II protein complex binding
          Process(es)
          humoral immune response B cell proliferation B cell activation
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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