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Invitrogen
CD20 Monoclonal Antibody (B9E9)
FIGURE: 1 / 4
CD20 Antibody (MA1-7634) in ICC/IF
Immunofluorescence analysis of CD20 was performed using 70 % confluent log phase Ramos cells. The cells were fixed with 4% paraformaldehyde for 5 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD20 Monoclonal Antibody (B9E9) (Product # MA1-7634) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # ... View More
Product Details
MA1-7634
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse,
Rat,
Rhesus monkey
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
B9E9
Immunogen
CD20, Human white blood cell fraction
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.2
Contains
0.01% sodium azide
Storage conditions
4°C short term, -80°C long term
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2250679
Product Specific Information
MA1-7634 detects CD20 from human and mouse samples.
MA1-7634 has been successfully used in ELISA, IF, flow cytometry, immunoprecipitation, and immunohistochemistry (frozen) procedures.
The MA1-7634 immunogen is a CD20 protein.
Store at 4ºC short term. Store at -80ºC long term.
Target Information
CD20 is a non-glycosylated surface phosphoprotein that has a molecular weight range of 33-37 kDa depending on the degree of phosphorylation. CD20 is expressed on mature and most malignant B cells, in a subpopulation of T lymphocytes and follicular dendritic cells. CD20 expression on B cells is synchronous with the expression of surface IgM and it regulates transmembrane calcium conductance, cell cycle progression and B-cell proliferation. CD20 is also associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change, and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following monoclonal antibody treatment. Diseases associated with CD20 dysfunction include Ms4a1-related common variable immune deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: APY; ATOPY; B-cell differentiation antigen Ly-44; B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; B-lymphocyte surface antigen B1; Bp35; CD20; CD20 antigen; CD20 receptor; Fc epsilon receptor I beta chain; Fc Fragment of IgE high affinity I receptor for beta polypeptide; FCER1B; High affinity immunoglobulin epsilon receptor subunit beta; IgE Fc receptor subunit beta; IGEL; IGER; IGHER; LEU16; Leukocyte surface antigen Leu-16; Ly44; Lymphocyte antigen 44; Membrane-spanning 4-domains subfamily A member 1; membrane-spanning 4-domains, subfamily A, member 1; membrane-spanning 4-domains, subfamily A, member 2; MGC3969
Gene Aliases: AA960661; B1; Bp35; CD20; CVID5; LEU-16; Ly-44; MS4A1; MS4A2; S7
UniProt ID: (Human) P11836, (Mouse) P19437
Entrez Gene ID: (Human) 931, (Mouse) 12482
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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