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Invitrogen
CD201 (EPCR) Monoclonal Antibody (eBio1560 (1560)), Functional Grade, eBioscience™
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CD201 (EPCR) Antibody (16-2012-83) in Flow
Staining of bEnd-3 cell line with 0.25 µg of Rat IgG2b Isotype Control Purified (Product # 14-4031-82) (open histogram) or 0.06 µg of Anti-Mouse CD201 (EPCR) Functional Grade Purified (filled histogram) followed by Anti-Rat IgG PE (Product # 12-4822).Total viable cells were used for analysis.
Product Details
16-2012-83
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Human,
Mouse,
Non-human primate
Host/Isotype
Rat
/ IgG2b, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
eBio1560 (1560)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_657696
Product Specific Information
Description: The eBio1560 monoclonal antibody reacts with mouse Endothelial Protein C Receptor (EPCR, CD201), a 25 kDa Type 1 transmembrane protein expressed on endothelial cells. EPCR exhibits sequence and structural homology with the MHC class I/CD1 family of proteins. EPCR is a ligand for Protein C and plays an important role in augmenting Protein C activation by the thrombin-thrombomodulin complex and in regulating blood coagulation and inflammation. Deletion of EPCR results in embryonic lethality, at least partically due to placental thrombosis.
Recently, it was demonstrated that EPCR expression identified cells in the bone marrow that are capable of hematopoietic reconstitution activity comparable to hematopoietic stem cells isolated with conventional methods. The eBio1560 monoclonal antibody can be used for the detection of these hematopoietic stem cells, however the eBiomRCR-16 monoclonal antibody should only be used for the detection of CD201 on endothelial cells.
Applications Reported: This eBio1560 (1560) antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining. The eBio1560 monoclonal antibody has been reported to block protein C activation by thrombin on cells expressing both thrombomodulin and CD201.
Applications Tested: This eBio1560 (1560) antibody has been tested by flow cytometric analysis of bEnd.3 cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest, particµLarly when analyzing expression on endothelial cells where CD201 expression is very high.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
Target Information
The protein encoded by this gene is a receptor for activated protein C, a serine protease activated by and involved in the blood coagulation pathway. The encoded protein is an N-glycosylated type I membrane protein that enhances the activation of protein C. Mutations in this gene have been associated with venous thromboembolism and myocardial infarction, as well as with late fetal loss during pregnancy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Activated protein C receptor; APC receptor; bA42O4.2; CD antigen CD201; CD201; Centrocyclin; Centrosomal protein CCD41; Endothelial cell protein C receptor; Endothelial protein C receptor
Gene Aliases: AI325044; Ccca; Ccd41; Epcr; Procr
UniProt ID: (Mouse) Q64695
Entrez Gene ID: (Mouse) 19124
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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