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Invitrogen
CD202b (TIE2) Monoclonal Antibody (33.1), eBioscience™
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CD202b (TIE2) Antibody (14-2029-80) in WB
Western blot was performed using CD202b (TIE2) Monoclonal Antibody (33.1), eBioscience™ (Product # 14-2029-82) and a 140 kDa band corresponding to TEK/TIE2 was observed across cell lines and tissues tested except U-87 MG and Mouse adipose which are reported to be negative. Whole cell extracts (30 µg lysate) of U-87 MG (Lane 1), HUVEC (Lane 2), Mouse lung (Lane 3), Mouse kidney (Lane 4), Mouse adipose (Lane 5) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitroce... View More
Product Details
14-2029-80
Applications
Tested Dilution
Publications
Product Specifications
Published species
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
33.1
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Storage conditions
4°C
Shipping conditions
Wet ice
RRID
AB_10854562
Product Specific Information
Description: This 33.1 monoclonal antibody reacts with human, mouse, rabbit, rat, pig, and zebrafish CD202b. Also known as Tie-2, this molecule is a member of the tyrosine kinase receptor family. CD202b is expressed on endothelial and a subset of hematopoietic cells and is believed to play a role in both angiogenesis and hematopoiesis during development of the mouse embryo. In fetal liver and adult bone marrow, Tie-2 is expressed by a subpopulation of hematopoietic progenitor cells characterized as Lineage markers-, c-Kit+, Sca1+ cells. Long-term multilineage repopulating cells were detected in Tie-2+, Lineage-, c-Kit+, Sca1+ cells but not in Tie-2-, Lineage-, c-Kit+, Sca1+ cells.
Applications Reported: This 33.1 antibody has been reported for use in western blotting.
Applications Tested: This 33.1 antibody has been tested by immunoblotting of reduced cell lysate prepared from the bEND3 cell line at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells. TEK signaling appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis, and defects in TEK are associated with inherited venous malformations. The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. Immunoblotting showed that TIE2 expression was increased by thyroid-stimulating hormone and agents that increased intracellular cAMP. HSCs expressing the receptor tyrosine kinase TIE2 are quiescent and antiapoptotic and comprise a side population of HSCs that adhere to osteoblasts in the bone marrow niche.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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