Invitrogen

CD202b (TIE2) Monoclonal Antibody (33.1), eBioscience™

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Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

14-2029-82

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

-
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Product Specifications

Species Reactivity

Human, Mouse, Pig, Rabbit, Rat

Published species

Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

33.1

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10870984

Product Specific Information

Description: This 33.1 monoclonal antibody reacts with human, mouse, rabbit, rat, pig, and zebrafish CD202b. Also known as Tie-2, this molecule is a member of the tyrosine kinase receptor family. CD202b is expressed on endothelial and a subset of hematopoietic cells and is believed to play a role in both angiogenesis and hematopoiesis during development of the mouse embryo. In fetal liver and adult bone marrow, Tie-2 is expressed by a subpopulation of hematopoietic progenitor cells characterized as Lineage markers-, c-Kit+, Sca1+ cells. Long-term multilineage repopulating cells were detected in Tie-2+, Lineage-, c-Kit+, Sca1+ cells but not in Tie-2-, Lineage-, c-Kit+, Sca1+ cells.

Applications Reported: This 33.1 antibody has been reported for use in western blotting.

Applications Tested: This 33.1 antibody has been tested by immunoblotting of reduced cell lysate prepared from the bEND3 cell line at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells. TEK signaling appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis, and defects in TEK are associated with inherited venous malformations. The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. Immunoblotting showed that TIE2 expression was increased by thyroid-stimulating hormone and agents that increased intracellular cAMP. HSCs expressing the receptor tyrosine kinase TIE2 are quiescent and antiapoptotic and comprise a side population of HSCs that adhere to osteoblasts in the bone marrow niche.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Angiopoietin-1 receptor; CD202b; CD202b antigen; EC 2.7.10; EC 2.7.10.1; Endothelial tyrosine kinase; endothelial-specific receptor tyrosine kinase; hTIE2; HYK; mTIE2; p140 TEK; STK1; TEK tyrosine kinase, endothelial; TEKT1; Tektin-1; Tunica interna endothelial cell kinase; Tyrosine kinase with Ig and EGF homology domains-2; tyrosine kinases that contain immunoglobulin-like loops and epidermal growth factor-similar domains 2; Tyrosine-protein kinase receptor TEK; Tyrosine-protein kinase receptor TIE-2

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Gene Aliases: AA517024; CD202B; Hyk; STK1; TEK; TIE-2; TIE2; VMCM; VMCM1

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UniProt ID: (Human) Q02763, (Mouse) Q02858

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Entrez Gene ID: (Rabbit) 100356675, (Pig) 396729, (Human) 7010, (Mouse) 21687, (Rat) 89804

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Function(s)
protein kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity receptor activity Ras guanyl-nucleotide exchange factor activity protein binding ATP binding growth factor binding nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups receptor signaling protein tyrosine kinase activity
Process(es)
MAPK cascade angiogenesis response to hypoxia positive regulation of protein phosphorylation endothelial cell proliferation positive regulation of endothelial cell proliferation endochondral ossification sprouting angiogenesis signal transduction transmembrane receptor protein tyrosine kinase signaling pathway cell-cell signaling heart development positive regulation of endothelial cell migration positive regulation of phosphatidylinositol 3-kinase signaling negative regulation of angiogenesis peptidyl-tyrosine phosphorylation organ regeneration regulation of establishment or maintenance of cell polarity substrate adhesion-dependent cell spreading negative regulation of apoptotic process regulation of vascular permeability response to peptide hormone positive regulation of GTPase activity positive regulation of phosphatidylinositol 3-kinase activity response to estrogen positive regulation of angiogenesis protein autophosphorylation Tie signaling pathway negative regulation of inflammatory response leukocyte migration protein oligomerization response to cAMP positive regulation of focal adhesion assembly positive regulation of protein kinase B signaling definitive hemopoiesis heart trabecula formation positive regulation of ERK1 and ERK2 cascade positive regulation of intracellular signal transduction positive regulation of actin cytoskeleton reorganization regulation of endothelial cell apoptotic process negative regulation of endothelial cell apoptotic process patterning of blood vessels vasculogenesis regulation of endothelial cell proliferation positive regulation of cytokine secretion involved in immune response protein phosphorylation cell-matrix adhesion phosphorylation single organismal cell-cell adhesion hemopoiesis regulation of cell migration positive regulation of vascular endothelial growth factor receptor signaling pathway response to retinoic acid positive regulation of peptidyl-serine phosphorylation intracellular signal transduction positive regulation of protein import into nucleus regulation of angiogenesis positive regulation of cell adhesion regulation of NIK/NF-kappaB signaling response to organic substance signal transduction by protein phosphorylation
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