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Invitrogen
CD205 Monoclonal Antibody (205yekta), NovaFluor™ Blue 660-120S, eBioscience™
FIGURE: 1 / 2
CD205 Antibody (M051T03B08-A) in Flow
C57BL/6 mouse bone marrow cells were unstained (left) or stained with 0.2 µg of CD205 Monoclonal Antibody, NovaFluor Blue 660-120S (right). All cells were co-stained with Ly-6G/Ly-6C (Gr-1) Monoclonal Antibody, eFluor 450 (Product # 48-5931-82). Total viable cells in the granulocyte gate were used for analysis, as determined by LIVE/DEAD Blue (Product # L34962). Data was acquired on a 5-laser Cytek Aurora and unmixed with autofluorescence extraction.
Product Details
M051T03B08-A
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
205yekta
Conjugate
Excitation/Emission Max
492/665 nm
View spectra
Form
Liquid
Concentration
0.1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
RRID
AB_3099224 (AB_2937343)
Product Specific Information
Description: The 205yekta antibody reacts with mouse CD205, also known as DEC-205 (dendritic and epithelial cells, 205 kDa). CD205 is an integral membrane glycoprotein involved in antigen uptake, trafficking and presentation that improves the induction of antigen-specific T cell immunity. CD205 is highly expressed by CD8+ dendritic cells (DCs) and also expressed at different levels by bone marrow Gr1+ cells, Langerhans cells, (BMDC) bone marrow derived DCs and thymic epithelial cells.
CD205 has been shown to bind to PE-Cy5.5 antibody and streptavidin conjugates; therefore, this format should not be used on CD205+ dendritic cells.
Each product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer .
Applications Reported: This 205yekta antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 205yekta antibody has been tested by flow cytometric analyis of mouse bone marrow cells. This can be used at less than or equal to 0.03 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
NovaFluor dyes are not compatible with DNA intercalating viability dyes. Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD) and DAPI. Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes.
This NovaFluor conjugate has been updated to ship with CellBlox Plus Blocking Buffer (Cat. No. (C001T06F01)). This buffer contains formulation improvements over CellBlox. CellBlox Plus Blocking Buffer is required for optimal staining with NovaFluor conjugates and should be used in all experiments where NovaFluor conjugates are used. Whenever possible, we recommend adding CellBlox Plus Blocking Buffer to antibody cocktails/master mixes prior to combining with cells. Add 5 µL per sample (regardless of the number of NovaFluors in your panel) to use the antibody cocktail as intended. For single-color controls, use 5 µL of CellBlox Blocking Buffer per 100 µL of cell sample containing 10^3 to 10^8 cells.
NovaFluor conjugates are based on Phiton™ technology utilizing novel nucleic acid dye structures that allow for engineered fluorescent signatures with consideration for spillover and spread impacts. Learn more
Excitation: 509 nm; Emission: 665 nm; Laser: 488 nm (Blue) Laser
Target Information
CD205 (LY25, Lymphocyte Antigen 75) is an integral membrane glycoprotein involved in antigen uptake, trafficking and presentation that improves the induction of antigen-specific T cell immunity. CD205 is highly expressed by CD8+ dendritic cells (DCs) and also expressed at different levels by bone marrow Gr1+ cells, Langerhans cells, (BMDC) bone marrow derived DCs and thymic epithelial cells. CD205 acts as an endocytic receptor that directs captured antigens to the extracellular space towards a specialized antigen processing region. Further, CD205 also prompts the reduced proliferation of B lymphocytes. Diseases associated with CD205 dysfunction include pneumonic plague and lipid pneumonia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CA-IX; CD205; DCL-1 fusion; DEC-205; gp200; Ly-75; Lymphocyte antigen 75; membrane antigen MN; P54/58N; pMW1; RP23-10L17.4
Gene Aliases: CD205; DEC-205; DEC205; Ly75
UniProt ID: (Mouse) Q60767
Entrez Gene ID: (Mouse) 17076
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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