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Invitrogen
CD209b (SIGN-R1) Monoclonal Antibody (eBio22D1 (22D1)), Alexa Fluor™ 488, eBioscience™
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FIGURE: 1 / 2
CD209b (SIGN-R1) Antibody (53-2093-82) in Flow
C57BL/6 mouse splenocytes were stained with CD11b Monoclonal Antibody, PE (Product # 12-0112-82) and 1.0 µg of Armenian Hamster IgG Isotype Control, Alexa Fluor 488 (Product # 53-4888-80) (left) or 1.0 µg of CD209b Monoclonal Antibody, Alexa Fluor 488 (right). Total viable cells were used for analysis, as determined by 7-AAD (Product # 00-6993-50).
Product Details
53-2093-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Armenian hamster
/ IgG
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
eBio22D1 (22D1)
Conjugate
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2802324
Product Specific Information
Description: The eBio22D1 monoclonal antibody reacts with mouse SIGNR1 (CD209b). SIGNR1 is a type II transmembrane C-type lectin that was identified in a search for mouse homologues of human DC-SIGN. It is expressed at high levels in splenic marginal zone macrophages and lymph node medullary macrophages, where it functions to uptake dextran polysaccharides, including the capsular polysaccharide of Streptococcus pneumoniae. It has also been demonstrated that SIGNR1 physically associates with TLR4/MD2, and it has been suggested that this association plays a role in recognition of LPS. Furthermore, recently it has been shown that SIGNR1 deficient mice have a defect in catabolism of the complement component C3, and that SIGNR1 binds directly to the complement C1 subcomponent, C1q to assemble a non-conventional C3 convertase. The eBio22D1 monoclonal antibody does not cross-react with the closely related SIGNR1, SIGNR2, SIGNR3 or SIGNR4.
Applications Reported: This eBio22D1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBio22D1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser
Target Information
SIGNR1 is a type II transmembrane C-type lectin that was identified in a search for mouse homologues of human DC-SIGN. It is expressed at high levels in splenic marginal zone macrophages and lymph node medullary macrophages, where it functions to uptake dextran polysaccharides, including the capsular polysaccharide of Streptococcus pneumoniae. It has also been demonstrated that SIGNR1 physically associates with TLR4/MD2, and it has been suggested that this association plays a role in recognition of LPS. Furthermore, recently it has been shown that SIGNR1 deficient mice have a defect in catabolism of the complement component C3, and that SIGNR1 binds directly to the complement C1 subcomponent, C1q to assemble a non-conventional C3 convertase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD209; CD209 antigen-like protein A; CD209 antigen-like protein B; DC SIGNR1; DC-SIGN; DC-SIGN-related protein 1; DC-SIGNR1; Dendritic cell-specific ICAM-3-grabbing non-integrin; MGC130443; OtB7
Gene Aliases: 1810030I22Rik; CD209; Cd209a; Cd209b; CDSIGN; CIRE; DC-SIGN; DC-SIGN1; DC-SIGNR1; Dcsign; mSIGNR1; OtB7; SIGN-R1; SIGNR1; SIGNR5
UniProt ID: (Mouse) Q91ZX1, (Mouse) Q8CJ88
Entrez Gene ID: (Mouse) 170786, (Mouse) 69165
detection of yeast
endocytosis
phagocytosis, recognition
detection of bacterium
positive regulation of tumor necrosis factor biosynthetic process
positive regulation of phagocytosis
regulation of gene expression
regulation of blood coagulation
regulation of T cell proliferation
viral entry into host cell
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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