Invitrogen

CD221 (IGF1R) Monoclonal Antibody (1H7), APC, eBioscience™

CD221 (IGF1R) Antibody in Flow Cytometry (Flow)

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CD221 (IGF1R) Antibody (17-8849-41) in Flow

Staining of the HeLa cell line with Mouse IgG1 kappa Isotype Control APC (Product # 17-4714-81) (blue histogram) or Anti-Human CD221 (Insulin-like Growth Factor-1 Receptor) APC (purple histogram). Total viable cells were used for analysis.

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Product Details

17-8849-41

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 3 publications 3 publications

Product Specifications

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

1H7

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Wet ice

AB_1907365

Product Specific Information

Description: This 1H7 monoclonal antibody reacts with human insulin-like growth factor-1 receptor (IGF-1R), also known as CD221. This receptor is a cell surface-expressed glycoprotein composed of two extracellular alpha subunits and two transmembrane beta subunits that possess tyrosine kinase activity. Expressed on nearly all cell types, this receptor binds IGF-I and IGF-II, as well as insulin. Ligand binding leads to activation of the PI3K/Akt and MAPK pathways, which mediate cell proliferation and survival. Many tumors and transformed cells display altered IGF-1R expression.

This monoclonal antibody has been reported to block binding of IGF-I and IGF-II to the IGF-1R.

Applications Reported: This 1H7 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 1H7 antibody has been pre-titrated and tested by flow cytometric analysis of the HeLa cell line. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IGF1R, also known as Insulin-like Growth Factor 1 (IGF-1) Receptor, is a receptor tyrosine kinase activated by IGF-1 and IGF-2. IGFR has been implicated in a variety of cancers. The protein is a tetramer consisting of two alpha, and two beta subunits. The alpha-subunits binds to insulin growth factor 1 with higher affinity than to insulin growth factor 2. Binding results in a conformational change, followed by autophosphorylation of IGF-1R at positions 1134, 1135, 1136. The activated IGF-1R in turn, causes phosphorylation of substrate proteins, followed by sequential activation of RAS, RAF, and mitogen-activated protein kinase isoforms ERK, p38, and JNK, leading to the transcription of genes that drive proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Antibody targeting of E3 ubiquitin ligases for receptor degradation.

Nature

Marei H,Tsai WK,Kee YS,Ruiz K,He J,Cox C,Sun T,Penikalapati S,Dwivedi P,Choi M,Kan D,Saenz-Lopez P,Dorighi K,Zhang P,Kschonsak YT,Kljavin N,Amin D,Kim I,Mancini AG,Nguyen T,Wang C,Janezic E,Doan A,Mai E,Xi H,Gu C,Heinlein M,Biehs B,Wu J,Lehoux I,Harris S,Comps-Agrar L,Seshasayee D,de Sauvage FJ,Grimmer M,Li J,Agard NJ,de Sousa E Melo F

Published figure using CD221 (IGF1R) monoclonal antibody (Product # 17-8849-42) in Flow Cytometry

Sat Oct 01 00:00:00 UTC 2022

Effects of IGF-1 on Proliferation, Angiogenesis, Tumor Stem Cell Populations and Activation of AKT and Hedgehog Pathways in Oral Squamous Cell Carcinoma.

International journal of molecular sciences

Ferreira Mendes JM,de Faro Valverde L,Torres Andion Vidal M,Paredes BD,Coelho P,Allahdadi KJ,Coletta RD,Souza BSF,Rocha CAG

17-8849 was used in Flow cytometry/Cell sorting to suggest that IGF-1 exerts pro-tumorigenic effects on SCC-4 cells by stimulating proliferation, migration, invasion and stemness, as well as activating AKT and hedgehog pathways.

Sat Sep 05 00:00:00 UTC 2020

Effects of IGF-1 on Proliferation, Angiogenesis, Tumor Stem Cell Populations and Activation of AKT and Hedgehog Pathways in Oral Squamous Cell Carcinoma.

International journal of molecular sciences

Ferreira Mendes JM,de Faro Valverde L,Torres Andion Vidal M,Paredes BD,Coelho P,Allahdadi KJ,Coletta RD,Souza BSF,Rocha CAG

Sat Sep 05 00:00:00 UTC 2020

Functional and differential proteomic analyses to identify platelet derived factors affecting ex vivo expansion of mesenchymal stromal cells.

BMC cell biology

Kinzebach S,Dietz L,Klüter H,Thierse HJ,Bieback K

17-8849 was used in Flow cytometry/Cell sorting to study the functionally bioactive factors which are seen to affect mesenchymal stromal cells expansion.

Wed Oct 30 00:00:00 UTC 2013

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