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          • Primary Antibodies ›
          • CD23 Antibodies

          Invitrogen

          CD23 Monoclonal Antibody (B3B4), Biotin, eBioscience™

          10 Published Figures
          26 References
          View all (149) CD23 antibodies

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          Datasheet
          Protocols
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          Datasheet
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          Cite CD23 Monoclonal Antibody (B3B4), Biotin, eBioscience™

          • Antibody Testing Data (1)
          • Published Figures (10)
          CD23 Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          CD23 Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 11

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          CD23 Antibody (13-0232-81) in Flow

          Surface staining of mouse splenocytes with Anti-Human/Mouse CD45R (B220) PE (Product # 12-0452-82) and Anti-Mouse CD23 FITC. Total viable cells were used for analysis. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          CD23 Antibody in Flow Cytometry (Flow)
          CD23 Antibody in Flow Cytometry (Flow)
          CD23 Antibody in Flow Cytometry (Flow)
          CD23 Antibody in Flow Cytometry (Flow)
          CD23 Antibody in Flow Cytometry (Flow)
          CD23 Antibody in Flow Cytometry (Flow)
          CD23 Antibody in Flow Cytometry (Flow)
          CD23 Antibody in Flow Cytometry (Flow)
          CD23 Antibody in Flow Cytometry (Flow)
          CD23 Antibody in Flow Cytometry (Flow)
          CD23 Antibody in Flow Cytometry (Flow)

          Product Details

          13-0232-81

          Applications
          Tested Dilution
          Publications

          Flow Cytometry (Flow)

          0.25 µg/test
          View 23 publications 23 publications

          Miscellaneous PubMed (MISC)

          -
          View 3 publications 3 publications
          Product Specifications

          Species Reactivity

          Mouse

          Published species

          Mouse

          Host/Isotype

          Rat / IgG2a, kappa

          Recommended Isotype Control

          Rat IgG2a kappa Isotype Control (eBR2a), Biotin, eBioscience™

          Class

          Monoclonal

          Type

          Antibody

          Clone

          B3B4

          Conjugate

          Biotin Biotin Biotin
          • Unconjugated
          • Brilliant UV 805
          • eFluor 450
          • eFluor 660
          • FITC
          • NovaFluor Blue 510
          • NovaFluor Blue 610-70S
          • NovaFluor Blue 660-120S
          • NovaFluor Red 685
          • NovaFluor Red 710
          • NovaFluor Red 725
          • NovaFluor Yellow 610
          • NovaFluor Yellow 660
          • NovaFluor Yellow 755
          • PE 
          • PE-Cyanine7
          • PerCP-eFluor 710
          • Super Bright 436
          • Super Bright 600
          • Super Bright 645
          • Request custom conjugation

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2

          Contains

          0.09% sodium azide

          Storage conditions

          4°C, store in dark, DO NOT FREEZE!

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_466392

          Product Specific Information

          Description: The B3B4 monoclonal antibody reacts with mouse CD23, a 45 kDa type II transmembrane glycoprotein. CD23 is expressed on resting conventional B cells, and its expression is modulated upon B-cell activation. B-1 cell lineage (CD5+ B cells) does not express CD23. Soluble forms of the antigen have been reported to be biologically active. CD23 is a low affinity receptor for IgE and is thought to play a role in the regulation of IgE response and B-cell activation. CD21 is thought to bind to CD23.

          Applications Reported: The B3B4 antibody has been reported for use in flow cytometric analysis.

          Applications Tested: The B3B4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Filtration: 0.2 µm post-manufacturing filtered.

          Target Information

          CD23 is a 45 kDa glycoprotein which is present on a subpopulation of freshly isolated peripheral blood and tonsil B cells and strongly expressed on EBV-transformed B lymphoblasts. The CD23 molecule is identical to the low affinity IgE receptor found on B cells. Expression of CD23 has been detected in neoplastic cells from cases of B cell chronic lymphocyctic leukaemia and some cases of centroblastic/centrocytic lymphoma. CD23 is present on a subpopulation of freshly isolated peripheral blood and tonsil B cells and strongly expressed on EBV-transformed B lymphoblasts. Functionally, CD23 is involved in B cell growth and differentiation, and IgE production. Further, CD23 has a soluble form that is a potent mitogenic factor. Diseases associated with CD23 dysfunction include chronic conjunctivitis and chronic lymphocytic leukemia.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: CD23; FC epsilon RII; Fc-epsilon-RII; FceRII; Low affinity immunoglobulin epsilon Fc receptor; low-affinity IgE receptor; Lymphocyte IgE receptor

          View more View less

          Gene Aliases: CD23; Fce2; Fcer2; Fcer2a; Ly-42

          View more View less

          UniProt ID: (Mouse) P20693

          View more View less

          Entrez Gene ID: (Mouse) 14128

          View more View less

          Function(s)
          IgE binding carbohydrate binding metal ion binding
          Process(es)
          positive regulation of humoral immune response mediated by circulating immunoglobulin positive regulation of nitric-oxide synthase activity positive regulation of killing of cells of other organism positive regulation of nitric-oxide synthase biosynthetic process
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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