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CD235a Monoclonal Antibody (CLB-ery-1 (AME-1)), FITC

2 Published Figures

9 References

View all (87) CD235a (Glycophorin A) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Published Figures (2)

CD235a Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 2

CD235a Antibody (MHGLA01) in Flow

Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie 2018 - Published figure using CD235a monoclonal antibody (Product # MHGLA01) in Flow Cytometry

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Product Details

MHGLA01

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-Dependent

View 9 publications 9 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Non-human primate

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

CLB-ery-1 (AME-1)

Immunogen

Human Glycophorin A (CD235a)

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Purification

purified

Storage buffer

PBS with BSA

Contains

0.1% sodium azide

Storage conditions

4°C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10372963

Product Specific Information

Commonly used, FITC conjugates provide relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility.

This antibody recognizes a papain-sensitive epitope in the extracellular domain of the sialoglycoprotein known as glycophorin A (CD235a).

Target Information

CD235a (Glycophorins A, GPA) is a single pass membrane sialoglycoprotein expressed in mature erythrocytes and erythroid precursor cells. CD235a is the carrier of blood group M and N specificities. CD235a provides cells with a large mucin-like that may serve as a barrier to cell fusion, minimizing aggregation between red blood cells in the circulation. CD235a has been shown to act as a receptor for Sandei virus, parvovirus, and Hsa, an Streptococcus adhesin.

Analyte Specific Reagent

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

The timing of gamma irradiation and its effect on cell-free and microvesicle-bound hemoglobin. The BEST collaborative study.

Transfusion

William N,Bicahlo B,Hansen A,de Korte D,Acker JP

MHGLA01 was used in Flow cytometry/Cell sorting to study how irradiation timing can alter the state of extracellular Hb, with "early" irradiation promoting an increased proportion of cell-free Hb as well as mechanical damage to the RBC membrane.

Fri Apr 01 00:00:00 UTC 2022

Characteristics of Extracellular Vesicles in Red Blood Concentrates Change with Storage Time and Blood Manufacturing Method.

Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie

Almizraq RJ,Holovati JL,Acker JP

MHGLA01 was used in Flow cytometry/Cell sorting to assess the impact of different blood manufacturing methods and duration of hypothermic storage on extracellular vesicle subpopulations.

Tue May 01 00:00:00 UTC 2018

Extracellular vesicle characteristics in stored red blood cell concentrates are influenced by the method of detection.

Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis

Almizraq RJ,Seghatchian J,Holovati JL,Acker JP

MHGLA01 was used in flow cytometry to evaluate the impact of different approaches to characterize extracellular vesicles in stored red blood cell products

Sat Apr 01 00:00:00 UTC 2017

Inhibition of G9a methyltransferase stimulates fetal hemoglobin production by facilitating LCR/γ-globin looping.

Blood

Krivega I,Byrnes C,de Vasconcellos JF,Lee YT,Kaushal M,Dean A,Miller JL

MHGLA01 was used in flow cytometry to investigate the effects of UNC0638 treatment on beta-globin gene expression using ex vivo differentiated CD34(+) erythroid progenitor cells

Thu Jul 30 00:00:00 UTC 2015

Differences in Rat and Human Erythrocytes Following Blood Component Manufacturing: The Effect of Additive Solutions.

Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie

da SilveiraCavalcante L,Acker JP,Holovati JL

MHGLA01 was used in flow cytometry to assess the effect of blood component manufacturing on the quality of rat red blood cells.

Fri May 01 00:00:00 UTC 2015

Erythroid-Specific Expression of LIN28A Is Sufficient for Robust Gamma-Globin Gene and Protein Expression in Adult Erythroblasts.

PloS one

Lee YT,de Vasconcellos JF,Byrnes C,Kaushal M,Rabel A,Tumburu L,Allwardt JM,Miller JL

MHGLA01 was used in flow cytometry to analyze gamma-globulin gene and protein expression in adult erythroblasts created by erythroid-specific expression of LIN28A

Mon Jul 04 00:00:00 UTC 2016

The effects of rejuvenation during hypothermic storage on red blood cell membrane remodeling.

Transfusion

Kurach JD,Almizraq R,Bicalho B,Acker JP,Holovati JL

MHGLA01 was used in flow cytometry to investigate the effect of cold rejuvenation treatment on RBC membrane storage lesions

Sun Jun 01 00:00:00 UTC 2014

Storage of red blood cells affects membrane composition, microvesiculation, and in vitro quality.

Transfusion

Almizraq R,Tchir JD,Holovati JL,Acker JP

MHGLA01 was used in flow cytometry to discuss red blood cell microparticles.

Tue Oct 01 00:00:00 UTC 2013

Identification of a cross-reacting, monoclonal anti-human CD233 antibody for identification and sorting of rhesus macaque erythrocytes.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Byrnes C,Terry Lee Y,Donahue RE,Miller JL

MHGLA01 was used in Flow cytometry/Cell sorting to identify a cross-reacting, monoclonal anti-human CD233 antibody for the identification and sorting of rhesus macaque erythrocytes.

Wed Feb 01 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: CD235; CD235a; erythroid-lineage-specific membrane sialoglycoprotein; glycophorin A (MN blood group); glycophorin A, GPA; glycophorin Erik; glycophorin MiI; glycophorin MiV; glycophorin SAT; glycophorin Sta type C; Glycophorin-A; HGNC:4702; HGpMiX; HGpStaC; Mi.V glycoprotein; Mi.V glycoprotein (24 AA); MN sialoglycoprotein; PAS-2; recombinant glycophorin A-B Miltenberger-DR; Sialoglycoprotein alpha

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Gene Aliases: CD235a; GPA; GPErik; GPSAT; GYPA; HGpMiV; HGpMiXI; HGpSta(C); MN; MNS; PAS-2

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UniProt ID: (Human) P02724

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Entrez Gene ID: (Human) 2993

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Function(s)

virus receptor activity identical protein binding

Process(es)

viral entry into host cell

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CD235a Monoclonal Antibody (CLB-ery-1 (AME-1)), FITC

CD235a Antibody (MHGLA01) in Flow

Product Details

MHGLA01

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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