Invitrogen

CD24 Monoclonal Antibody (SN3)

CD24 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 16

CD24 Antibody (MA5-11828) in ICC/IF

Immunofluorescent analysis of CD24 (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD24 monoclonal antibody (Product # MA5-11828) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjug... View More

CD24 Antibody in Immunohistochemistry (Frozen) (IHC (F))

Product Image Gallery

CD24 Antibody — Analysis of the role of CD24 in Zika-virus infected neuroblastoma cells. A) Western blot analysis of siRNA-mediated knock-down of CD24 expression in IMR-32 cells. Samples include Negative Control siRNA and CD24 siRNA. B) Western blot analysis of the expression of Envelope protein and NS1 (Non-Structural 1) protein in IMR-32 cells after siRNA-mediated knock-down of CD24 expression, 96 hours after Zika infection (MOI = 10). Samples include control cells treated with non-infected conditioned media and infected IMR-32 cells transfected with either Negative Control siRNA or CD24 siRNA. C) Western blot analysis of CD24 expression in the human neuroblastoma cell line SK-N-AS, comparing wild type (WT) to stably selected Vector Only (VO), CD24 variant 1 (V1), and CD24 variant 7 (V7). D) Western blot analysis of Zika NS1 protein expression 96 hours after Zika infection in CD24-stably expressing SK-N-AS cells, comparing wild type (WT) to stably selected Vector Only (VO), CD24 variant 1 (V1), and CD24 variant 7 (V7). GAPDH was used as a load control for all experiments. All results are representative of the combined data of experiments performed in triplicate. {KD}

Product Details

MA5-11828

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 7 publications 7 publications

Immunohistochemistry (IHC)

View 15 publications 15 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

1:10-1:100

View 4 publications 4 publications

Flow Cytometry (Flow)

Assay-dependent

View 9 publications 9 publications

Miscellaneous PubMed (MISC)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

SN3

Immunogen

Cell membrane antigen preparation isolated from NALM-1 human pre-B leukemia cell line

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10983158

Product Specific Information

MA5-11828 targets CD24 in FACS, IHC (F), ICC/IF and WB applications and shows reactivity with Human and Mouse samples. This antibody detects a few non-specific bands in BAF-3 cell lysates.

The MA5-11828 immunogen is cell membrane antigen preparation isolated from NALM-1 human pre-B leukemia cell line.

Target Information

CD24 is a two-chain glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed at multiple stages of B-cell development, beginning with the bone marrow pro-B-cell compartment and continuing through mature, surface Ig positive B-cells. Plasma cell expression is very low or negative. CD24 is also expressed on the majority of B-lineage acute lymphoblastic leukemias, B-cell CCLs and B-cell non-Hodgkin's lymphomas. CD24 may play a role in regulation of B-cell proliferation and maturation, and control of autoimmunity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Advanced High-Content-Screening Applications of Clonogenicity in Cancer.

SLAS discovery : advancing life sciences R & D

Esquer H,Zhou Q,Abraham AD,LaBarbera DV

MA5-11828 was used in Immunocytochemistry to present a HCS method to quantify the number and size of colonies in 2D and 3D culture models and to distinguish colonies based on fluorescent markers using an Opera Phenix high-content-screening system.

Sat Aug 01 00:00:00 UTC 2020

Transmissible gastroenteritis virus targets Paneth cells to inhibit the self-renewal and differentiation of Lgr5 intestinal stem cells via Notch signaling.

Cell death & disease

Wu A,Yu B,Zhang K,Xu Z,Wu D,He J,Luo J,Luo Y,Yu J,Zheng P,Che L,Mao X,Huang Z,Wang L,Zhao J,Chen D

Published figure using CD24 monoclonal antibody (Product # MA5-11828) in Western Blot

Mon Jan 20 00:00:00 UTC 2020

Highly versatile cancer photoimmunotherapy using photosensitizer-conjugated avidin and biotin-conjugated targeting antibodies.

Cancer cell international

Shirasu N,Shibaguchi H,Yamada H,Kuroki M,Yasunaga S

MA5-11828 was used in Flow Cytometry to demonstrate that AvIR-mediated PIT can greatly broaden the applicable range of target specificity, with feasibility of efficacious and integrative control of CSC and its microenvironment.

Mon Apr 11 00:00:00 UTC 2022

Zika virus as an oncolytic treatment of human neuroblastoma cells requires CD24.

PloS one

Mazar J,Li Y,Rosado A,Phelan P,Kedarinath K,Parks GD,Alexander KA,Westmoreland TJ

Published figure using CD24 monoclonal antibody (Product # MA5-11828) in Western Blot

Thu Jan 17 00:00:00 UTC 2019

A CD44v⁺ subpopulation of breast cancer stem-like cells with enhanced lung metastasis capacity.

Cell death & disease

Hu J,Li G,Zhang P,Zhuang X,Hu G

Published figure using CD24 monoclonal antibody (Product # MA5-11828) in Flow Cytometry

Thu Mar 16 00:00:00 UTC 2017

A CD44v⁺ subpopulation of breast cancer stem-like cells with enhanced lung metastasis capacity.

Cell death & disease

Hu J,Li G,Zhang P,Zhuang X,Hu G

Published figure using CD24 monoclonal antibody (Product # MA5-11828) in Flow Cytometry

Thu Mar 16 00:00:00 UTC 2017

Secreted Frizzled-related protein 4 (sFRP4) chemo-sensitizes cancer stem cells derived from human breast, prostate, and ovary tumor cell lines.

Scientific reports

Deshmukh A,Kumar S,Arfuso F,Newsholme P,Dharmarajan A

MA5-11828 was used in Immunocytochemistry-immunoflourescence to investigate molecular signals essential to sustain cancer stem cells and assess their activity in the presence of secreted frizzled-related protein 4 alone or in combination with chemotherapeutic drugs.

Tue May 23 00:00:00 UTC 2017

Increased Steady-State Memory B Cell Subsets Among High-Risk Participants in an HIV Vaccine Trial.

AIDS research and human retroviruses

Keefer MC,Zheng B,Rosenberg AF,Kobie JJ

MA511828 was used in flow cytometry to investigate how differences in B cell populations may contribute to HIV susceptibility

Mon Dec 18 00:00:00 UTC 2017

CD24 Expression May Play a Role as a Predictive Indicator and a Modulator of Cisplatin Treatment Response in Head and Neck Squamous Cellular Carcinoma.

PloS one

Modur V,Joshi P,Nie D,Robbins KT,Khan AU,Rao K

MA511828 was used in immunohistochemistry - paraffin section and western blot to evaluate the use of CD24 expression as a predictive indicator to identify patients with head and neck cancer that will react favorably to platinum-based therapy

Fri Jul 14 00:00:00 UTC 2017

Differential expression of ligands for NKG2D and DNAM-1 receptors by epithelial ovarian cancer-derived exosomes and its influence on NK cell cytotoxicity.

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

Labani-Motlagh A,Israelsson P,Ottander U,Lundin E,Nagaev I,Nagaeva O,Dehlin E,Baranov V,Mincheva-Nilsson L

MA5-11828 was used in immunocytochemistry and western blot to characterize the influence on NK cell cytotoxicity by differential expression of ligands for DNAM-1 and NKG2D receptors by epithelial ovarian cancer-derived exosomes

Fri Apr 01 00:00:00 UTC 2016

The Hedgehog signaling pathway is associated with poor prognosis in breast cancer patients with the CD44+/CD24‑ phenotype.

Molecular medicine reports

Zhao H,Tang H,Xiao Q,He M,Zhao L,Fu Y,Wu H,Yu Z,Jiang Q,Yan Y,Jin F,Wei M

MA5-11828 was used in Immunohistochemistry to investigate the expression of Patched (PTCH) and Gli1, which are the main components of the Hh signaling pathway, as well as the expression of cluster of differentiation (CD)44/CD24, which are markers for breast CSCs, in 266 patients with breast cancer.

Thu Dec 01 00:00:00 UTC 2016

Humoral Dysregulation Associated with Increased Systemic Inflammation among Injection Heroin Users.

PloS one

Piepenbrink MS,Samuel M,Zheng B,Carter B,Fucile C,Bunce C,Kiebala M,Khan AA,Thakar J,Maggirwar SB,Morse D,Rosenberg AF,Haughey NJ,Valenti W,Keefer MC,Kobie JJ

MA511828 was used in flow cytometry to characterize B cells and antibody repertoires from injection drug users

Mon Jul 31 00:00:00 UTC 2017

Diversity, cellular origin and autoreactivity of antibody-secreting cell population expansions in acute systemic lupus erythematosus.

Nature immunology

Tipton CM,Fucile CF,Darce J,Chida A,Ichikawa T,Gregoretti I,Schieferl S,Hom J,Jenks S,Feldman RJ,Mehr R,Wei C,Lee FE,Cheung WC,Rosenberg AF,Sanz I

MA5-11828 was used in flow cytometry to examine the antibody repertoire of patients with acute systemic lupus erythematosus.

Wed Jul 01 00:00:00 UTC 2015

Addition of niclosamide to palladium(II) saccharinate complex of terpyridine results in enhanced cytotoxic activity inducing apoptosis on cancer stem cells of breast cancer.

Bioorganic & medicinal chemistry

Karakas D,Cevatemre B,Aztopal N,Ari F,Yilmaz VT,Ulukaya E

MA5-11828 was used in flow cytometry to assess the effects of palladium(II) and niclosamide on the Wnt signaling pathway associated with breast cancer stem cells

Tue Sep 01 00:00:00 UTC 2015

The clinicopathological and prognostic significance of CD24, CD44, CD133, ALDH1 expressions in invasive ductal carcinoma of the breast: CD44/CD24 expression in breast cancer.

Pathology, research and practice

Kapucuoğlu N,Bozkurt KK,Başpınar Ş,Koçer M,Eroğlu HE,Akdeniz R,Akçil M

MA5-11828 was used in immunohistochemistry to evaluate the expression of CD24, CD44, CD133 and ALDH1 in invasive ductal carcinoma of the breast

Thu Oct 01 00:00:00 UTC 2015

CD24 Overexpression Is Associated with Poor Prognosis in Luminal A and Triple-Negative Breast Cancer.

PloS one

Kwon MJ,Han J,Seo JH,Song K,Jeong HM,Choi JS,Kim YJ,Lee SH,Choi YL,Shin YK

MA5-11828 was used in western blot to elucidate a poor prognosis in triple-negative breast cancer and luminal A by CD24 overexpression

Wed Jun 01 00:00:00 UTC 2016

Fibromatosis-like metaplastic carcinoma of the breast has a claudin-low immunohistochemical phenotype.

Virchows Archiv : an international journal of pathology

Rito M,Schmitt F,Pinto AE,André S

MA5-11828 was used in immunohistochemistry to study the claudin expression in fibromatosis-like metaplastic carcinoma of the breast

Fri Aug 01 00:00:00 UTC 2014

CD24 and CD44 in salivary gland pleomorphic adenoma and in human salivary gland morphogenesis: differential markers of glandular structure or stem cell indicators?

Histopathology

Ianez RC,Coutinho-Camillo CM,Buim ME,Pinto CA,Soares FA,Lourenço SV

MA5-11828 was used in immunohistochemistry to study whether CD24 and CD44 expression in salivary glands can be used as markers of cancer stem cells

Sat Jun 01 00:00:00 UTC 2013

Genomic and phenotypic profiles of two Brazilian breast cancer cell lines derived from primary human tumors.

Oncology reports

Corrêa NC,Kuasne H,Faria JA,Seixas CC,Santos IG,Abreu FB,Nonogaki S,Rocha RM,Aparecida Borges Silva G,Gobbi H,Rogatto SR,Goes AM,Gomes DA

MA5-11828 was used in immunohistochemistry - paraffin section to characterize Brazilian breast cancer cell lines

Mon Apr 01 00:00:00 UTC 2013

Profiling of normal and malignant breast tissue show CD44high/CD24low phenotype as a predominant stem/progenitor marker when used in combination with Ep-CAM/CD49f markers.

BMC cancer

Ghebeh H,Sleiman GM,Manogaran PS,Al-Mazrou A,Barhoush E,Al-Mohanna FH,Tulbah A,Al-Faqeeh K,Adra CN

MA5-11828 was used in flow cytometry to determine the phenotype and function of stem/progenitor cells in normal mammary epithelial cell populations and their malignant counterparts

Fri Jun 14 00:00:00 UTC 2013

Autoimmunity, intestinal lymphoid hyperplasia, and defects in mucosal B-cell homeostasis in patients with PTEN hamartoma tumor syndrome.

Gastroenterology

Heindl M,Händel N,Ngeow J,Kionke J,Wittekind C,Kamprad M,Rensing-Ehl A,Ehl S,Reifenberger J,Loddenkemper C,Maul J,Hoffmeister A,Aretz S,Kiess W,Eng C,Uhlig HH

MA5-11828 was used in Flow Cytometry to examine the effects of Phosphatase And Tensin Homolog Deleted On Chromosome 10 hamartoma tumour syndrome on the immune system.

Tue May 01 00:00:00 UTC 2012

Multiparameter flow cytometry and bioanalytics for B cell profiling in systemic lupus erythematosus.

Methods in molecular biology (Clifton, N.J.)

Kaminski DA,Wei C,Rosenberg AF,Lee FE,Sanz I

MA5-11828 was used in flow cytometry to generate specialized B cell reagent panels to characterize B cell disorders.

Thu Jan 03 00:00:00 UTC 2013

CD24 expression as a marker for predicting clinical outcome in human gliomas.

Journal of biomedicine & biotechnology

Deng J,Gao G,Wang L,Wang T,Yu J,Zhao Z

MA5-11828 was used in western blot to study the prognostic value of CD24 expression in human gliomas

Fri Sep 07 00:00:00 UTC 2012

Acetaminophen-induced differentiation of human breast cancer stem cells and inhibition of tumor xenograft growth in mice.

Biochemical pharmacology

Takehara M,Hoshino T,Namba T,Yamakawa N,Mizushima T

MA5-11828 was used in immunohistochemistry to screen compounds able to induce differentiation of cancer stem cells

Sun May 01 00:00:00 UTC 2011

Different prognostic significance of CD24 and CD44 expression in breast cancer according to hormone receptor status.

Breast (Edinburgh, Scotland)

Kim HJ,Kim MJ,Ahn SH,Son BH,Kim SB,Ahn JH,Noh WC,Gong G

MA5-11828 was used in immunohistochemistry to study the clinical significance of CD24 and CD44 expression in breast cancer.

Tue Feb 01 00:00:00 UTC 2011

Characterization of sphere-propagating cells with stem-like properties from DU145 prostate cancer cells.

Biochimica et biophysica acta

Rybak AP,He L,Kapoor A,Cutz JC,Tang D

MA5-11828 was used in immunohistochemistry to study sphere-propagating cells with stem-like properties from DU145 prostate cancer cells

Sun May 01 00:00:00 UTC 2011

CD24 is a novel predictor for poor prognosis of hepatocellular carcinoma after surgery.

Clinical cancer research : an official journal of the American Association for Cancer Research

Yang XR,Xu Y,Yu B,Zhou J,Li JC,Qiu SJ,Shi YH,Wang XY,Dai Z,Shi GM,Wu B,Wu LM,Yang GH,Zhang BH,Qin WX,Fan J

MA5-11828 was used in immunohistochemistry to study the role of CD24 in tumor invasion and its prognostic significance in hepatocellular carcinoma

Tue Sep 01 00:00:00 UTC 2009

Association of breast cancer stem cells identified by aldehyde dehydrogenase 1 expression with resistance to sequential Paclitaxel and epirubicin-based chemotherapy for breast cancers.

Clinical cancer research : an official journal of the American Association for Cancer Research

Tanei T,Morimoto K,Shimazu K,Kim SJ,Tanji Y,Taguchi T,Tamaki Y,Noguchi S

MA5-11828 was used in immunohistochemistry to study whether cancer stem cells are clinically significant for resistance to chemotherapy in human breast cancers

Mon Jun 15 00:00:00 UTC 2009

CD24 expression is a novel prognostic factor in esophageal squamous cell carcinoma.

Annals of surgical oncology

Sano A,Kato H,Sakurai S,Sakai M,Tanaka N,Inose T,Saito K,Sohda M,Nakajima M,Nakajima T,Kuwano H

MA5-11828 was used in western blot to study the prognostic value of CD24 overexpression in esophageal squamous cell carcinoma

Sun Feb 01 00:00:00 UTC 2009

A new population of cells lacking expression of CD27 represents a notable component of the B cell memory compartment in systemic lupus erythematosus.

Journal of immunology (Baltimore, Md. : 1950)

Wei C,Anolik J,Cappione A,Zheng B,Pugh-Bernard A,Brooks J,Lee EH,Milner EC,Sanz I

MA5-11828 was used in flow cytometry to discuss the frequency and contribution of CD27- memory B cells to systemic lupus erythematosus

Tue May 15 00:00:00 UTC 2007

The role of extracellular factors in human metastatic chordoma cell growth in vitro.

Spine

Ostroumov E,Hunter CJ

MA5-11828 was used in western blot to study the role of the extracellular microenvironment in human metastatic chordoma cell growth in vitro

Sat Dec 15 00:00:00 UTC 2007

Laser capture microdissection-microarray analysis of focal segmental glomerulosclerosis glomeruli.

Nephron. Experimental nephrology

Bennett MR,Czech KA,Arend LJ,Witte DP,Devarajan P,Potter SS

MA5-11828 was used in immunohistochemistry to analyse the focal segmental glomerulosclerosis glomeruli by laser capture microdissection-microarray method

Tue Oct 02 00:00:00 UTC 2007

CD24 expression is a prognostic factor in intrahepatic cholangiocarcinoma.

Cancer letters

Su MC,Hsu C,Kao HL,Jeng YM

MA5-11828 was used in immunohistochemistry to study CD24 expression as a prognostic factor in intrahepatic cholangiocarcinoma

Sat Apr 08 00:00:00 UTC 2006

Identification and purification of classical Hodgkin cells from lymph nodes by flow cytometry and flow cytometric cell sorting.

American journal of clinical pathology

Fromm JR,Kussick SJ,Wood BL

MA5-11828 was used in flow cytometry to evaluate the use of flow cytometry to identify the Hodgkin and Reed-Sternberg cells of classical Hodgkin lymphoma

Wed Nov 01 00:00:00 UTC 2006

Cytoplasmic CD24 expression in colorectal cancer independently correlates with shortened patient survival.

Clinical cancer research : an official journal of the American Association for Cancer Research

Weichert W,Denkert C,Burkhardt M,Gansukh T,Bellach J,Altevogt P,Dietel M,Kristiansen G

MA5-11828 was used in immunohistochemistry to study the prognostic value of cytoplasmic CD24 expression in colorectal cancer

Thu Sep 15 00:00:00 UTC 2005

Expression profiling of microdissected matched prostate cancer samples reveals CD166/MEMD and CD24 as new prognostic markers for patient survival.

The Journal of pathology

Kristiansen G,Pilarsky C,Wissmann C,Kaiser S,Bruemmendorf T,Roepcke S,Dahl E,Hinzmann B,Specht T,Pervan J,Stephan C,Loening S,Dietel M,Rosenthal A

MA5-11828 was used in immunohistochemistry to profile the expression patterns of microdissected prostate cancer samples to identify new prognostic markers

Tue Feb 01 00:00:00 UTC 2005

Molecular and prognostic distinction between serous ovarian carcinomas of varying grade and malignant potential.

Oncogene

Meinhold-Heerlein I,Bauerschlag D,Hilpert F,Dimitrov P,Sapinoso LM,Orlowska-Volk M,Bauknecht T,Park TW,Jonat W,Jacobsen A,Sehouli J,Luttges J,Krajewski M,Krajewski S,Reed JC,Arnold N,Hampton GM

MA5-11828 was used in immunohistochemistry to distinguish low malignant potential serous ovarian carcinomas from invasive counterparts

Thu Feb 03 00:00:00 UTC 2005

CD24 expression is a significant predictor of PSA relapse and poor prognosis in low grade or organ confined prostate cancer.

The Prostate

Kristiansen G,Pilarsky C,Pervan J,Stürzebecher B,Stephan C,Jung K,Loening S,Rosenthal A,Dietel M

MA5-11828 was used in immunohistochemistry to study the prognostic value of CD24 expression in low grade or organ confined prostate cancer

Sun Feb 01 00:00:00 UTC 2004

Bioinformatics

Protein Aliases: CD24; CD24 antigen (small cell lung carcinoma cluster 4 antigen); cluster of differentiation 24; FLJ22950; FLJ43543; heat stable antigen; HSA; Ly-52; Lymphocyte antigen 52; M1/69-J11D heat stable antigen; MGC75043; Nectadrin; R13-Ag; Signal transducer CD24; Small cell lung carcinoma cluster 4 antigen; X62 heat stable antigen

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Gene Aliases: CD24; CD24A; HSA; Ly-52; nectadrin

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UniProt ID: (Human) P25063, (Mouse) P24807

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Entrez Gene ID: (Human) 100133941, (Mouse) 12484

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Function(s)

signal transducer activity protein binding protein kinase binding protein tyrosine kinase activator activity carbohydrate binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD24 Monoclonal Antibody (SN3)

CD24 Antibody (MA5-11828) in ICC/IF

Product Details

MA5-11828

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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