Invitrogen

CD244 (2B4) Monoclonal Antibody (M053)

View all (2) CD244 (2B4) antibodies

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Product Details

MA5-47659

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500
-

Immunocytochemistry (ICC/IF)

1:200
-

ELISA (ELISA)

Assay-dependent
-

Immunoprecipitation (IP)

1:100
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

M053

Immunogen

Clone (M053) was generated from a recombinant protein that included the extracellular region of human CD244 protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein G

Storage buffer

PBS with 1mg/mL BSA, 50% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2942640

Product Specific Information

Clone M053 mouse monoclonal antibody detects a 110 kDa* protein on SDS-PAGE "Native" or denatured immunoblots of human THP1 monocytes. The antibody prefers the native CD244 protein, but also detects denatured CD244. The antibody works for western blot, immunoprecipitation, immunocytochemistry, and ELISA capture.

Target Information

CD244 (Natural killer (NK) cell receptor 2B4/SLAMF4) is an Ig superfamily signaling lymphocyte activation molecule (SLAM) receptor. Like all SLAM family receptors, it has an extracellular segment with two immunoglobulin (Ig)-like domains, and a cytoplasmic domain containing four immunoreceptor tyrosine-based switch motifs. CD244 does not act as a selfligand similar to other SLAM family receptors. It binds CD48, a transmembrane receptor ubiquitously expressed on hematopoietic cells. CD244 activity is controlled by the presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Downstream signaling involves predominantly VAV1, and, to a lesser degree, INPP5D/SHIP1 and CBL. Activation of CD244 stimulates NK cell cytotoxicity, production of IFN-gamma and granule exocytosis. CD244 is involved in the regulation of CD8+ T-cell proliferation, and inhibits inflammatory responses in dendritic cells (DCs). In cancers, CD244 shows increased expression in intratumoral DCs and myeloid suppressor cells, and anti-CD244 therapies may increase infiltrating T-cells and impair tumor growth.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CD244; CD244 molecule, natural killer cell receptor 2B4; h2B4; NAIL; Natural killer cell receptor 2B4; NK cell activation inducing ligand NAIL; NK cell activation-inducing ligand; NK cell type I receptor protein 2B4; NKR2B4; Signaling lymphocytic activation molecule 4; SLAM family member 4; SLAMF4

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Gene Aliases: 2B4; CD244; NAIL; NKR2B4; Nmrk; SLAMF4

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UniProt ID: (Human) Q9BZW8

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Entrez Gene ID: (Human) 51744

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