Invitrogen

CD244 (2B4) Monoclonal Antibody (M085)

View all (2) CD244 (2B4) antibodies

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Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

MA5-47660

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunocytochemistry (ICC/IF)

1:200
-

ELISA (ELISA)

Assay-dependent
-

Immunoprecipitation (IP)

1:100
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

M085

Immunogen

Clone (M085) was generated from a recombinant protein that included the extracellular region of human CD244 protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein G

Storage buffer

PBS with 1mg/mL BSA, 50% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2942641

Product Specific Information

Clone M085 mouse monoclonal antibody detects a 110 kDa* protein on SDS-PAGE "Native" immunoblots of human THP1 monocytes. The antibody detects only the native CD244 protein, and the aldehyde-fixed CD244 protein. The antibody works for native western blot, immunoprecipitation, immunocytochemistry, and ELISA capture.

Target Information

CD244 (Natural killer (NK) cell receptor 2B4/SLAMF4) is an Ig superfamily signaling lymphocyte activation molecule (SLAM) receptor. Like all SLAM family receptors, it has an extracellular segment with two immunoglobulin (Ig)-like domains, and a cytoplasmic domain containing four immunoreceptor tyrosine-based switch motifs. CD244 does not act as a selfligand similar to other SLAM family receptors. It binds CD48, a transmembrane receptor ubiquitously expressed on hematopoietic cells. CD244 activity is controlled by the presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Downstream signaling involves predominantly VAV1, and, to a lesser degree, INPP5D/SHIP1 and CBL. Activation of CD244 stimulates NK cell cytotoxicity, production of IFN-gamma and granule exocytosis. CD244 is involved in the regulation of CD8+ T-cell proliferation, and inhibits inflammatory responses in dendritic cells (DCs). In cancers, CD244 shows increased expression in intratumoral DCs and myeloid suppressor cells, and anti-CD244 therapies may increase infiltrating T-cells and impair tumor growth.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CD244; CD244 molecule, natural killer cell receptor 2B4; h2B4; NAIL; Natural killer cell receptor 2B4; NK cell activation inducing ligand NAIL; NK cell activation-inducing ligand; NK cell type I receptor protein 2B4; NKR2B4; Signaling lymphocytic activation molecule 4; SLAM family member 4; SLAMF4

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Gene Aliases: 2B4; CD244; NAIL; NKR2B4; Nmrk; SLAMF4

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UniProt ID: (Human) Q9BZW8

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Entrez Gene ID: (Human) 51744

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