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          • Primary Antibodies ›
          • CD25 Antibodies

          Invitrogen

          CD25 Monoclonal Antibody (PC61.5.3)

          39 References
          View all (229) CD25 antibodies
          Datasheet
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          Datasheet
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          Cite CD25 Monoclonal Antibody (PC61.5.3)

          • Antibody Testing Data (2)
          CD25 Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          CD25 Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 2

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          CD25 Antibody (MA5-17812) in Flow

          Flow cytometric analysis of Con A Activated T-Cell Blasts stained using anti-CD25/IL-2R alpha chain Monoclonal Antibody (Product # MA5-17812) at a 0.5 µg/10^6 cells dilution. The Isotype control was a Rat IgG1 {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          CD25 Antibody in Flow Cytometry (Flow)
          CD25 Antibody in Flow Cytometry (Flow)

          Product Details

          MA5-17812

          Applications
          Tested Dilution
          Publications

          Immunohistochemistry (IHC)

          -
          View 1 publication 1 publication

          Flow Cytometry (Flow)

          0.5-1 µg/1x10^6 cells
          View 20 publications 20 publications

          Immunoprecipitation (IP)

          Assay-dependent
          -

          Functional Assay (Functional)

          Assay-dependent
          -

          Miscellaneous PubMed (MISC)

          -
          View 18 publications 18 publications
          Product Specifications

          Species Reactivity

          Mouse

          Published species

          Mouse

          Host/Isotype

          Rat / IgG1

          Class

          Monoclonal

          Type

          Antibody

          Clone

          PC61.5.3

          Immunogen

          B6.1 CTL Cell line
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated
          • Biotin 
          • FITC
          • PE 
          • Request custom conjugation

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Protein G

          Storage buffer

          PBS

          Contains

          0.02% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2539196

          Product Specific Information

          MA5-17812 targets CD25/IL-2R alpha chain in FACS, FN, IHC (F), and IP applications and shows reactivity with Mouse samples.

          The MA5-17812 immunogen is b6.1 CTL Cell line.

          Target Information

          CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor. The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. IL2 is primarily produced by mature T cells. IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: CD25; IL 2 RA; IL 2 receptor; IL 2R; IL 2R subunit alpha; il-2 receptor alpha; IL-2 receptor subunit alpha; IL-2-RA; IL-2R alpha chain; IL-2R subunit alpha; IL2 RA; IL2 RA antibody; IL2 receptor; IL2 receptor subunit alpha; IL2-RA; IL2R subunit alpha; Il2ra antibody; interleukin-2 receptor alpha; Interleukin-2 receptor subunit alpha; Interleukin2 receptor subunit alpha; p55; p55 chain; sIL 2R; soluble IL 2 receptor

          View more View less

          Gene Aliases: CD25; Il2r; Il2ra; Ly-43

          View more View less

          UniProt ID: (Mouse) P01590

          View more View less

          Entrez Gene ID: (Mouse) 16184

          View more View less

          Function(s)
          interleukin-2 receptor activity protein binding drug binding interleukin-2 binding
          Process(es)
          inflammatory response to antigenic stimulus activation-induced cell death of T cells inflammatory response immune response Notch signaling pathway interleukin-2-mediated signaling pathway positive regulation of T cell proliferation positive regulation of activated T cell proliferation negative regulation of T cell proliferation T cell homeostasis positive regulation of T cell differentiation regulation of T cell homeostatic proliferation negative regulation of lymphocyte proliferation negative regulation of defense response to virus negative regulation of inflammatory response negative regulation of immune response
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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