Invitrogen

CD253 (TRAIL) Monoclonal Antibody (N2B2), eBioscience™

2 Published Figures

11 References

View all (40) TRAIL (CD253) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (2)

CD253 (TRAIL) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 3

CD253 (TRAIL) Antibody (14-5951-82) in Flow

Staining of mouse TRAIL-transfected cells with 0.5 µg of Rat IgG2a kappa Isotype Control Purified (Product # 14-4321-82) (open histogram) or 0.25 µg of Anti-Mouse CD253 (TRAIL) Purified (filled histogram) followed by F (ab')2 Anti-Rat IgG PE (Product # 12-4822). Total viable cells were used for analysis.

CD253 (TRAIL) Antibody in Neutralization (Neu)

CD253 (TRAIL) Antibody in Inhibition Assays (IA)

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Product Details

14-5951-82

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

1 µg/test

View 4 publications 4 publications

Immunoprecipitation (IP)

Assay-Dependent

Neutralization (Neu)

Assay-Dependent

View 4 publications 4 publications

Functional Assay (Functional)

Assay-Dependent

View 1 publication 1 publication

Inhibition Assays (IA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

N2B2

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_467743

Product Specific Information

Description: The N2B2 monoclonal antibody reacts with mouse TNF-related apoptosis-inducing ligand (TRAIL), a member of the TNF superfamily. TRAIL is not detected on the surface of freshly isolated T, B, or NK cells, but can be induced preferentially on CD3^- NK1.1^+ NK cells after stimulation with IL-2 or IL-15. N2B2 inhibits IL-2- or IL-15-activated NK cell cytotoxicity against mouse fibrosarcoma L929 target cells.

Applications Reported: N2B2 can be used in flow cytometric analysis, and blocking of TRAIL in functional assays. (Please use Functional Grade purified N2B2, Product # 16-5951, in functional assays).

Applications Tested: The N2B2 antibody has been tested by flow cytometric analysis of mouse TRAIL transfected cells and can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

TRAIL is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. TRAIL preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. TRAIL binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of TRAIL protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3. Receptors for TRAIL include two death domain containing receptors, death receptor 4 (DR4) and DR5, as well as two decoy receptors, DcR1 and DcR2, lacking the intracellular signaling death domain. TRAIL is a type II membrane protein and expressed in a variety of human tissues. Like TNF and Fas ligand, TRAIL induces apoptosis and NF-kB activation in many tissues and cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CD8⁺ T cells maintain killing of MHC-I-negative tumor cells through the NKG2D-NKG2DL axis.

Nature cancer

Lerner EC,Woroniecka KI,D'Anniballe VM,Wilkinson DS,Mohan AA,Lorrey SJ,Waibl-Polania J,Wachsmuth LP,Miggelbrink AM,Jackson JD,Cui X,Raj JA,Tomaszewski WH,Cook SL,Sampson JH,Patel AP,Khasraw M,Gunn MD,Fecci PE

Published figure using CD253 (TRAIL) monoclonal antibody (Product # 14-5951-82) in Inhibition Assays

Fri Sep 01 00:00:00 UTC 2023

Osteoclast-associated receptor blockade prevents articular cartilage destruction via chondrocyte apoptosis regulation.

Nature communications

Park DR,Kim J,Kim GM,Lee H,Kim M,Hwang D,Lee H,Kim HS,Kim W,Park MC,Shim H,Lee SY

14-5951 was used in Neutralization experiments to show osteoclast-associated receptor as a catabolic regulator of osteoarthritis pathogenesis.

Fri Aug 28 00:00:00 UTC 2020

Plasmacytoid dendritic cells orchestrate TLR7-mediated innate and adaptive immunity for the initiation of autoimmune inflammation.

Scientific reports

Takagi H,Arimura K,Uto T,Fukaya T,Nakamura T,Choijookhuu N,Hishikawa Y,Sato K

14-5951 was used in Flow cytometry/Cell sorting to investigate the role of plasmacytoid dendritic cells in autoimmunity, showing that they orchestrate TLR7-mediated immune response and initiate autoimmune inflammation.

Thu Apr 14 00:00:00 UTC 2016

NKp46+ Innate Lymphoid Cells Dampen Vaginal CD8 T Cell Responses following Local Immunization with a Cholera Toxin-Based Vaccine.

PloS one

Luci C,Bekri S,Bihl F,Pini J,Bourdely P,Nouhen K,Malgogne A,Walzer T,Braud VM,Anjuère F

14-5951 was used in Flow cytometry/Cell sorting to show that vaginal NKp46+ Innate lymphoid cells dampen vaccine-induced CD8 T cell responses generated after local immunization with a Cholera Toxin-Based Vaccine.

Thu Jun 23 00:00:00 UTC 2016

Vaccine-elicited CD8+ T cells cure mesothelioma by overcoming tumor-induced immunosuppressive environment.

Cancer research

Tan Z,Zhou J,Cheung AK,Yu Z,Cheung KW,Liang J,Wang H,Lee BK,Man K,Liu L,Yuen KY,Chen Z

14-5951 was used in Flow cytometry/Cell sorting to demonstrate that antigen-specific CD8(+) T-cell immune response correlates with the elimination of mesothelioma by a model PD-1-based DNA vaccine.

Sat Nov 01 00:00:00 UTC 2014

Vaccine-elicited CD8+ T cells cure mesothelioma by overcoming tumor-induced immunosuppressive environment.

Cancer research

Tan Z,Zhou J,Cheung AK,Yu Z,Cheung KW,Liang J,Wang H,Lee BK,Man K,Liu L,Yuen KY,Chen Z

14-5951 was used in Flow cytometry/Cell sorting to demonstrate that antigen-specific CD8(+) T-cell immune response correlates with the elimination of mesothelioma by a model PD-1-based DNA vaccine.

Sat Nov 01 00:00:00 UTC 2014

RIPK1 promotes death receptor-independent caspase-8-mediated apoptosis under unresolved ER stress conditions.

Cell death & disease

Estornes Y,Aguileta MA,Dubuisson C,De Keyser J,Goossens V,Kersse K,Samali A,Vandenabeele P,Bertrand MJ

Published figure using CD253 (TRAIL) monoclonal antibody (Product # 14-5951-82) in Neutralization

Thu Dec 04 00:00:00 UTC 2014

Fasting enhances TRAIL-mediated liver natural killer cell activity via HSP70 upregulation.

PloS one

Dang VT,Tanabe K,Tanaka Y,Tokumoto N,Misumi T,Saeki Y,Fujikuni N,Ohdan H

14-5951 was used in Flow cytometry/Cell sorting to investigate the molecular mechanisms underlying the enhancement of natural killer cell function by fasting in mice.

Mon Jun 29 00:00:00 UTC 2015

Intratumoral delivery of low doses of anti-CD40 mAb combined with monophosphoryl lipid a induces local and systemic antitumor effects in immunocompetent and T cell-deficient mice.

Journal of immunotherapy (Hagerstown, Md. : 1997)

Van De Voort TJ,Felder MA,Yang RK,Sondel PM,Rakhmilevich AL

14-5951 was used in Neutralization experiments to assess the immunomodulatory and antitumour synergy between an agonistic anti-CD40 monoclonal antibody and monophosphoryl lipid A in mice.

Tue Jan 01 00:00:00 UTC 2013

c-FLIP maintains tissue homeostasis by preventing apoptosis and programmed necrosis.

Science signaling

Piao X,Komazawa-Sakon S,Nishina T,Koike M,Piao JH,Ehlken H,Kurihara H,Hara M,Van Rooijen N,Schütz G,Ohmuraya M,Uchiyama Y,Yagita H,Okumura K,He YW,Nakano H

14-5951 was used in Neutralization experiments to investigate the role of c-Flip in postnatal intestine and liver development, showing that homeostasis is maintained by inhibiting apoptosis and programmed necrosis.

Tue Dec 18 00:00:00 UTC 2012

TRAIL limits excessive host immune responses in bacterial meningitis.

The Journal of clinical investigation

Hoffmann O,Priller J,Prozorovski T,Schulze-Topphoff U,Baeva N,Lunemann JD,Aktas O,Mahrhofer C,Stricker S,Zipp F,Weber JR

14-5951 was used in Immunocytochemistry to demonstrate that TRAIL may act as a negative regulator of acute CNS inflammation during bacterial meningitis.

Sun Jul 01 00:00:00 UTC 2007

Bioinformatics

Protein Aliases: CD253; CD253 antigen; Protein TRAIL; TN; TNF superfamily member 10; TNF-related apoptosis inducing ligand; TNF-related apoptosis-inducing ligand; TRAIL protein; TRAIL/APO2L; Tumor necrosis factor ligand superfamily member 10

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Gene Aliases: A330042I21Rik; AI448571; APO-2L; Ly81; TL2; Tnfsf10; Trail

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UniProt ID: (Mouse) P50592

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Entrez Gene ID: (Mouse) 22035

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Function(s)

cytokine activity tumor necrosis factor receptor binding tumor necrosis factor receptor superfamily binding

Process(es)

apoptotic process immune response positive regulation of apoptotic process positive regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of release of cytochrome c from mitochondria positive regulation of extrinsic apoptotic signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD253 (TRAIL) Monoclonal Antibody (N2B2), eBioscience™

CD253 (TRAIL) Antibody (14-5951-82) in Flow

Product Details

14-5951-82

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Neutralization (Neu)

Functional Assay (Functional)

Inhibition Assays (IA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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