Invitrogen

CD253 (TRAIL) Monoclonal Antibody (RIK-2), Functional Grade, eBioscience™

CD253 (TRAIL) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 2

CD253 (TRAIL) Antibody (16-9927-38) in Flow

Staining of human Trail transfected cells with Anti-Human CD253 (TRAIL) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

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Product Details

16-9927-38

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

1 µg/test

View 5 publications 5 publications

Neutralization (Neu)

Assay-Dependent

View 1 publication 1 publication

Functional Assay (Functional)

Assay-Dependent

Inhibition Assays (IA)

View 1 publication 1 publication

Product Specifications

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Functional Grade, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

RIK-2

Conjugate

Functional Grade Functional Grade Functional Grade

Form

Liquid

Concentration

1 mg/mL

Storage conditions

4°C

Shipping conditions

Wet ice

AB_2866394

Product Specific Information

Description: The RIK-2 monoclonal antibody reacts with human TNF-related apoptosis-inducing ligand (TRAIL), a member of the TNF superfamily. TRAIL is not expressed by resting human cells but has been shown to be induced under certain activation conditions. Iin addition it is expressed on several human tumor lines. Interaction of TRAIL with its ligand, Apo-2, induces apoptosis. RIK-2 blocks TRAIL-induced apoptosis.

Applications Reported: The RIK-2 antibody has been reported for use in flow cytometric analysis. It has also been reported in blocking of TRAIL in functional assays.

Applications Tested: The RIK-2 antibody has been tested for blocking of staining with fluorochrome conjugated RIK-2 in flow cytometric analysis of human TRAIL transfected cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

TRAIL is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. TRAIL preferentially induces apoptosis in transformed and tumor cells, but does not appear to kill normal cells although it is expressed at a significant level in most normal tissues. TRAIL binds to several members of TNF receptor superfamily including TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly also to TNFRSF11B/OPG. The activity of this protein may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG that cannot induce apoptosis. The binding of TRAIL protein to its receptors has been shown to trigger the activation of MAPK8/JNK, caspase 8, and caspase 3. Receptors for TRAIL include two death domain containing receptors, death receptor 4 (DR4) and DR5, as well as two decoy receptors, DcR1 and DcR2, lacking the intracellular signaling death domain. TRAIL is a type II membrane protein and expressed in a variety of human tissues. Like TNF and Fas ligand, TRAIL induces apoptosis and NF-kB activation in many tissues and cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Fluid shear stress enhances natural killer cell's cytotoxicity toward circulating tumor cells through NKG2D-mediated mechanosensing.

APL bioengineering

Hu B,Xin Y,Hu G,Li K,Tan Y

16-9927-82 was used in Flow cytometry/Cell sorting to demonstrate that human epithelial cells recapitulate maturation of ILC from a stringent systemic human ILCP population, but only when the organoid-associated stromal cells are depleted.

Fri Sep 01 00:00:00 UTC 2023

Canonical TGF-β Signaling Pathway Represses Human NK Cell Metabolism.

Journal of immunology (Baltimore, Md. : 1950)

Zaiatz-Bittencourt V,Finlay DK,Gardiner CM

16-9927 was used in Flow cytometry/Cell sorting to suggest that pharmacological inhibition of TGF-β could provide a metabolic advantage to NK cells that is likely to result in improved functional responses.

Fri Jun 15 00:00:00 UTC 2018

Dengue virus activates membrane TRAIL relocalization and IFN-α production by human plasmacytoid dendritic cells in vitro and in vivo.

PLoS neglected tropical diseases

Gandini M,Gras C,Azeredo EL,Pinto LM,Smith N,Despres P,da Cunha RV,de Souza LJ,Kubelka CF,Herbeuval JP

16-9927 was used in Immunocytochemistry-immunofluorescence to characterise the activation of human plasmacytoid dendritic cells during DENV-2 infection in vivo, and their antiviral role in vitro.

Mon Dec 16 00:00:00 UTC 2013

MADD knock-down enhances doxorubicin and TRAIL induced apoptosis in breast cancer cells.

PloS one

Turner A,Li LC,Pilli T,Qian L,Wiley EL,Setty S,Christov K,Ganesh L,Maker AV,Li P,Kanteti P,Das Gupta TK,Prabhakar BS

16-9927 was used in Flow cytometry/Cell sorting to investigate how strategies to decrease MADD expression in breast cancer may be utilised to increase tumour cell sensitivity to TRAIL and doxorubicin induced apoptosis.

Wed Aug 28 00:00:00 UTC 2013

TRAIL death receptor-4, decoy receptor-1 and decoy receptor-2 expression on CD8+ T cells correlate with the disease severity in patients with rheumatoid arthritis.

BMC musculoskeletal disorders

Bisgin A,Terzioglu E,Aydin C,Yoldas B,Yazisiz V,Balci N,Bagci H,Gorczynski RM,Akdis CA,Sanlioglu S

Published figure using CD253 (TRAIL) monoclonal antibody (Product # 16-9927-82) in Flow Cytometry

Fri Aug 27 00:00:00 UTC 2010

Bystander macrophage apoptosis after Mycobacterium tuberculosis H37Ra infection.

Infection and immunity

Kelly DM,ten Bokum AM,O'Leary SM,O'Sullivan MP,Keane J

16-9927 was used in Blocking experiments to investigate bystander apoptosis of macrophages not infected with M.tuberculosis.

Tue Jan 01 00:00:00 UTC 2008

HIV turns plasmacytoid dendritic cells (pDC) into TRAIL-expressing killer pDC and down-regulates HIV coreceptors by Toll-like receptor 7-induced IFN-alpha.

Proceedings of the National Academy of Sciences of the United States of America

Hardy AW,Graham DR,Shearer GM,Herbeuval JP

16-9927 was used in Flow cytometry/Cell sorting to show that infectious and noninfectious HIV-1 virions induce activation of plasmacytoid dendritic cells (pDC) into TRAIL-expressing, IFN-producing killer pDCs.

Tue Oct 30 00:00:00 UTC 2007

Anti-HIV state but not apoptosis depends on IFN signature in CD4+ T cells.

Journal of immunology (Baltimore, Md. : 1950)

Audigé A,Urosevic M,Schlaepfer E,Walker R,Powell D,Hallenberger S,Joller H,Simon HU,Dummer R,Speck RF

16-9927-82 was used in Inhibition Assays to gain insights into the molecular mechanisms underlying early host responses to HIV in the CD4(+) T cell target population.

Wed Nov 01 00:00:00 UTC 2006

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CD253 (TRAIL) Monoclonal Antibody (RIK-2), Functional Grade, eBioscience™

CD253 (TRAIL) Antibody (16-9927-38) in Flow

Product Details

16-9927-38

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Neutralization (Neu)

Functional Assay (Functional)

Inhibition Assays (IA)

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Performance Guarantee

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