Invitrogen

CD254 (RANK Ligand) Monoclonal Antibody (12A380)

1 Published Figure
2 References
View all (68) RANKL (RANK Ligand) antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA1-20347

Applications
Tested Dilution
Publications

Western Blot (WB)

4-6 µg/mL
-

Immunohistochemistry (IHC)

-
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:250
View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:10-1:500
-

Flow Cytometry (Flow)

1:10-1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

12A380

Immunogen

A bacterially expressed fusion protein containing amino acid residues 1-317 of mouse TRANCE.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_561794

Product Specific Information

MA1-20347 detects sRANKL in Human and Mouse samples.

Target Information

This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CD254; H-RANK-L; M-Rank-L; ODF; OPG ligand; OPGL; Osteoclast differentiation factor; Osteoprotegerin ligand; rank-l; RANKL; receptor activator of NF-kappaB ligand; receptor activator of nuclear factor kappa B ligand; Receptor activator of nuclear factor kappa-B ligand; RP11-86N24.2; srankl; TNF-related activation-induced cytokine; TRANCE; tumor necrosis factor (ligand) superfamily member 11; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand 6B; Tumor necrosis factor ligand superfamily member 11; tumor necrosis factor-related activation-induced cytokine

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Gene Aliases: CD254; hRANKL2; Ly109l; ODF; OPG; OPGL; OPTB2; RANKL; sOdf; TNFSF11; TNLG6B; TRANCE

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UniProt ID: (Human) O14788, (Mouse) O35235

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Entrez Gene ID: (Human) 8600, (Mouse) 21943

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Function(s)
cytokine activity tumor necrosis factor receptor binding tumor necrosis factor receptor superfamily binding protein binding
Process(es)
ossification osteoclast proliferation monocyte chemotaxis immune response activation of JUN kinase activity organ morphogenesis cytokine-mediated signaling pathway osteoclast differentiation tumor necrosis factor-mediated signaling pathway mammary gland epithelial cell proliferation positive regulation of homotypic cell-cell adhesion paracrine signaling positive regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of MAP kinase activity bone resorption positive regulation of osteoclast differentiation positive regulation of bone resorption positive regulation of transcription from RNA polymerase II promoter positive regulation of T cell activation positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of NF-kappaB transcription factor activity protein homooligomerization positive regulation of corticotropin-releasing hormone secretion positive regulation of protein kinase B signaling calcium ion homeostasis mammary gland alveolus development ERK1 and ERK2 cascade positive regulation of fever generation by positive regulation of prostaglandin secretion TNFSF11-mediated signaling pathway positive regulation of ERK1 and ERK2 cascade via TNFSF11-mediated signaling positive regulation of intracellular signal transduction positive regulation of osteoclast development multicellular organism development cell differentiation positive regulation of phosphorylation regulation of osteoclast differentiation positive regulation of JNK cascade lymph node development
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