Invitrogen

CD262 (DR5) Monoclonal Antibody (MD5-1), Biotin, eBioscience™

CD262 (DR5) Antibody in Flow Cytometry (Flow)

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CD262 (DR5) Antibody (13-5883-82) in Flow

Staining of non-transfected (left) and mouse DR5 transfected (right) BHK cells with 0.06 µg of Golden Syrian Hamster IgG Isotype Control Biotin (Product # 13-4914-81) (open histogram) or 0.06 µg of Anti-Mouse CD262 (DR5) Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.

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Product Details

13-5883-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.125 µg/test

View 5 publications 5 publications

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MD5-1

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_466748

Product Specific Information

Description: The MD5-1 monoclonal antibody reacts with mouse DR5, also known as TRAIL-R2, Apo2, TRICK2, and KILLER. DR5 binds to TRAIL, activates NF-kappaB, and induces TRAIL-mediated apoptosis. DR5 mRNA is expressed broadly by normal tissue and human antigen is also expressed by some tumor cell lines.

Applications Reported: The MD5-1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The MD5-1 antibody has been tested by flow cytometric analysis of mouse DR5-transfected cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

DR5 (Apo2, TRAIL-R2, TRICK2, KILLER) is a recently identified death domain containing receptor for TRAIL, which mediates TRAIL induced apoptosis. DR5 is a member of the TNF-receptor superfamily, and contains an intracellular death domain. DR5 can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces an apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by DR5. Two transcript variants encoding different isoforms of DR5 and one non-coding transcript have been found. Overexpression of DR5 induces apoptosis and activates NF-kappa-B. DR5 is expressed in a number of cell types, and to particularly high levels in lymphocytes and spleen. Diseases associated with DR5 dysfunction include squamous cell carcinoma, and diffuse infiltrative lymphocytosis syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

IRF-5 Promotes Cell Death in CD4 T Cells during Chronic Infection.

Cell reports

Fabié A,Mai LT,Dagenais-Lussier X,Hammami A,van Grevenynghe J,Stäger S

13-5883-81 was used in Flow Cytometry to demonstrate that IRF-5 is increasingly expressed in interferon (IFN)-γ+ CD4 T cells over the course of L. donovani infection.

Tue Jul 31 00:00:00 UTC 2018

Trypanosoma brucei Co-opts NK Cells to Kill Splenic B2 B Cells.

PLoS pathogens

Frenkel D,Zhang F,Guirnalda P,Haynes C,Bockstal V,Radwanska M,Magez S,Black SJ

13-5883 was used in Flow cytometry/Cell sorting to investigate the mechanism by which natural killer cells in Trypanosoma brucei infected mice kill B cells, suppress humoral immunity and induce early mortality.

Fri Jul 01 00:00:00 UTC 2016

Tissues in different anatomical sites can sculpt and vary the tumor microenvironment to affect responses to therapy.

Molecular therapy : the journal of the American Society of Gene Therapy

Devaud C,Westwood JA,John LB,Flynn JK,Paquet-Fifield S,Duong CP,Yong CS,Pegram HJ,Stacker SA,Achen MG,Stewart TJ,Snyder LA,Teng MW,Smyth MJ,Darcy PK,Kershaw MH

13-5883 was used in Flow cytometry/Cell sorting to explore the relationship between tumour microenvironments and efficacy of immunotherapy.

Wed Jan 01 00:00:00 UTC 2014

Interleukin-2 rescues helpless effector CD8+ T cells by diminishing the susceptibility to TRAIL mediated death.

Immunology letters

Wolkers MC,Bensinger SJ,Green DR,Schoenberger SP,Janssen EM

13-5883 was used in Flow cytometry/Cell sorting to demonstrate that IL-2R signalling at the time of reactivation can play an important role in restoring CD8(+) T cell function.

Fri Sep 30 00:00:00 UTC 2011

CD11c+ dendritic cells and B cells contribute to the tumoricidal activity of anti-DR5 antibody therapy in established tumors.

Journal of immunology (Baltimore, Md. : 1950)

Haynes NM,Hawkins ED,Li M,McLaughlin NM,Hämmerling GJ,Schwendener R,Winoto A,Wensky A,Yagita H,Takeda K,Kershaw MH,Darcy PK,Smyth MJ

13-5883 was used in Flow cytometry/Cell sorting to investigate the cellular and molecular mechanisms contributing to the antitumour activity of selective antagonism of death receptors 4 and 5, showing that CD11c+ dendritic cells and B cells contribute to the tumoricidal activity of anti-DR5 antibody therapy.

Thu Jul 01 00:00:00 UTC 2010

Induction of tumor-specific T cell immunity by anti-DR5 antibody therapy.

The Journal of experimental medicine

Takeda K,Yamaguchi N,Akiba H,Kojima Y,Hayakawa Y,Tanner JE,Sayers TJ,Seki N,Okumura K,Yagita H,Smyth MJ

Mon Feb 16 00:00:00 UTC 2004

Bioinformatics

Protein Aliases: CD262; Death receptor 5; dr-5; KILLER/DR5 TRAIL death-inducing receptor; MK; sCD262; soluble CD262; Tumor necrosis factor receptor superfamily member 10B

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Gene Aliases: DR5; KILLER; Ly98; MK; Tnfrsf10b; TRAILR2; TRICK2A; TRICK2B; TRICKB

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UniProt ID: (Mouse) Q9QZM4

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Entrez Gene ID: (Mouse) 21933

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Function(s)

protease binding transcription factor binding cysteine-type endopeptidase activator activity involved in apoptotic process TRAIL binding

Process(es)

apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process signal transduction extrinsic apoptotic signaling pathway via death domain receptors TRAIL-activated apoptotic signaling pathway regulation of apoptotic process extrinsic apoptotic signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD262 (DR5) Monoclonal Antibody (MD5-1), Biotin, eBioscience™

CD262 (DR5) Antibody (13-5883-82) in Flow

Product Details

13-5883-82

Flow Cytometry (Flow)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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