Invitrogen

CD265 (RANK) Monoclonal Antibody (R12-31), PE, eBioscience™

4 Published Figures

7 References

View all (43) RANK (CD265) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (4)

CD265 (RANK) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 5

CD265 (RANK) Antibody (12-6612-82) in Flow

Staining of CD265-transfected cells with 0.5 µg of Rat IgG2a kappa Isotype Control PE (Product # 12-4321-80) (open histogram) or 0.5 µg of Anti-Mouse CD265 (RANK) PE (filled histogram). Total viable cells were used for analysis.

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Product Details

12-6612-82

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Flow Cytometry (Flow)

1 µg/test

View 6 publications 6 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

R12-31

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_466134

Product Specific Information

Description: The R12-31 monoclonal antibody reacts with mouse RANK (Receptor activator of NF-kappaB), a recently cloned member of the TNFR superfamily with no significant homology to other members of this family. RANK ligand (RANKL/TRANCE/ OPGL) binds to RANK on dendritic cells, upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in dendritic cell survival. The cytoplasmic domain of RANK interacts with TRAF2, TRAF5 and TRAF6. Overexpression of RANK activates NF-kappaB and c-Jun-terminal kinase (JNK) pathways. Recent studies have shown that RANK interaction with TRAF6 activates NF-kappaB, whereas JNK activation is mediated through binding of RANK to TRAF2.

Applications Reported: The R12-31 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This R12-31 antibody has been tested by flow cytometric analysis of mouse RANK-transfected cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

RANK is a member of the tumor necrosis factor receptor (TNFR) family. The full length mouse RANK cDNA encodes a type I transmembrane protein with an extracellular and a cytoplasmic domain. The extracellular domain contains two potential N-linked glycosylation sites. RANK shares significant amino acid homology with other members of the TNFR family in its extracellular four cysteine-rich repeats. Human and murine RANK share 81% amino acid identity in their extracellular domains.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Antenatal Corticosteroid Therapy Attenuates Angiogenesis Through Inhibiting Osteoclastogenesis in Young Mice.

Frontiers in cell and developmental biology

Chai Y,Su J,Hong W,Zhu R,Cheng C,Wang L,Zhang X,Yu B

12-6612 was used in Flow cytometry/Cell sorting to indicate Ezh2 expression of osteoclast precursors is suppressed after ACT, which reduced the pre-osteoclast number and PDGF-BB secretion, thus inhibiting type H vessel formation and ACT-associated low bone mineralization.

Sat Jan 02 00:00:00 UTC 2021

Antenatal Corticosteroid Therapy Attenuates Angiogenesis Through Inhibiting Osteoclastogenesis in Young Mice.

Frontiers in cell and developmental biology

Chai Y,Su J,Hong W,Zhu R,Cheng C,Wang L,Zhang X,Yu B

Sat Jan 02 00:00:00 UTC 2021

Interleukin-21 promotes osteoclastogenesis in RAW264.7 cells through the PI3K/AKT signaling pathway independently of RANKL.

International journal of molecular medicine

Xing R,Zhang Y,Li C,Sun L,Yang L,Zhao J,Liu X

12-6612-82 was used in Western Blotting to examine the osteoclastogenic activity of interleukin-21 in RAW264.7 cells in the absence of receptor activator of nuclear factor-κB ligand.

Sat Oct 01 00:00:00 UTC 2016

STAT3 restrains RANK- and TLR4-mediated signalling by suppressing expression of the E2 ubiquitin-conjugating enzyme Ubc13.

Nature communications

Zhang H,Hu H,Greeley N,Jin J,Matthews AJ,Ohashi E,Caetano MS,Li HS,Wu X,Mandal PK,McMurray JS,Moghaddam SJ,Sun SC,Watowich SS

12-6612 was used in Flow cytometry/Cell sorting to investigate the mechanism by which STAT3 curbs pro-inflammatory cytokine production mediated by NF-κB signalling, showing that it restrains signalling by suppressing expression of Ubc13.

Mon Dec 15 00:00:00 UTC 2014

Inhibition of Ca²⁺/calmodulin-dependent protein kinase kinase 2 stimulates osteoblast formation and inhibits osteoclast differentiation.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Cary RL,Waddell S,Racioppi L,Long F,Novack DV,Voor MJ,Sankar U

12-6612 was used in Flow cytometry/Cell sorting to reveal a novel function for CaMKK2 in bone remodelling and highlight the potential for therapeutic inhibition as a bone anabolic strategy.

Mon Jul 01 00:00:00 UTC 2013

Inhibition of Ca²⁺/calmodulin-dependent protein kinase kinase 2 stimulates osteoblast formation and inhibits osteoclast differentiation.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Cary RL,Waddell S,Racioppi L,Long F,Novack DV,Voor MJ,Sankar U

12-6612 was used in Flow cytometry/Cell sorting to reveal a novel function for CaMKK2 in bone remodelling and highlight the potential for therapeutic inhibition as a bone anabolic strategy.

Mon Jul 01 00:00:00 UTC 2013

Resolvin E1 regulates osteoclast fusion via DC-STAMP and NFATc1.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Zhu M,Van Dyke TE,Gyurko R

12-6612 was used in Flow cytometry/Cell sorting to evaluate the contribution of the proresolution mediator resolvin E1 (RvE1) on the resolution phase during inflammatory bone disease.

Thu Aug 01 00:00:00 UTC 2013

Lineage-committed osteoclast precursors circulate in blood and settle down into bone.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Muto A,Mizoguchi T,Udagawa N,Ito S,Kawahara I,Abiko Y,Arai A,Harada S,Kobayashi Y,Nakamichi Y,Penninger JM,Noguchi T,Takahashi N

12-6612 was used in Flow cytometry/Cell sorting to demonstrate that receptor activator of NF-κB-positive cells isolated from bone marrow and peripheral blood possess characteristics of quiescent osteoclast precursors in mice.

Thu Dec 01 00:00:00 UTC 2011

Phospholipase Cgamma2 mediates RANKL-stimulated lymph node organogenesis and osteoclastogenesis.

The Journal of biological chemistry

Chen Y,Wang X,Di L,Fu G,Chen Y,Bai L,Liu J,Feng X,McDonald JM,Michalek S,He Y,Yu M,Fu YX,Wen R,Wu H,Wang D

Published figure using CD265 (RANK) monoclonal antibody (Product # 12-6612-82) in Flow Cytometry

Fri Oct 24 00:00:00 UTC 2008

Bioinformatics

Protein Aliases: CD265; ODFR; Osteoclast differentiation factor receptor; receptor activator of NF-kappaB; Receptor activator of NF-KB; Tumor necrosis factor receptor superfamily member 11A

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Gene Aliases: Ly109; mRANK; ODFR; OFE; Rank; Tnfrsf11a; TRANCE-R

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UniProt ID: (Mouse) O35305

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Entrez Gene ID: (Mouse) 21934

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Function(s)

transmembrane signaling receptor activity tumor necrosis factor-activated receptor activity protein binding cytokine binding metal ion binding

Process(es)

ossification adaptive immune response multicellular organism development programmed cell death osteoclast differentiation response to lipopolysaccharide tumor necrosis factor-mediated signaling pathway response to cytokine response to tumor necrosis factor positive regulation of JUN kinase activity lymph node development positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of NF-kappaB transcription factor activity circadian temperature homeostasis mammary gland alveolus development response to interleukin-1 positive regulation of fever generation by positive regulation of prostaglandin secretion TNFSF11-mediated signaling pathway positive regulation of ERK1 and ERK2 cascade via TNFSF11-mediated signaling

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD265 (RANK) Monoclonal Antibody (R12-31), PE, eBioscience™

CD265 (RANK) Antibody (12-6612-82) in Flow

Product Details

12-6612-82

Western Blot (WB)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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