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Invitrogen
CD265 (RANK) Monoclonal Antibody (R12-31), eBioscience™
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FIGURE: 1 / 5
CD265 (RANK) Antibody (14-6612-82) in WB
International journal of molecular medicine 2016 -
Figure 1 Effect of interleukin-21 (IL-21) on the expression of IL-21 receptor (IL-21R) and receptor activator of nuclear factor-kappaB (RANK) in RAW264.7 cells. RAW264.7 cells were cultured with various concentrations of IL-21 (0, 1, 10, 20 and 40 ng/ml) for 48 h. (A and C) The mRNA expression of RANK and IL-21R was determined by RT-PCR. (B and D) The protein expression of RANK and IL-21R was determined by ... View More
Product Details
14-6612-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
R12-31
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_468179
Product Specific Information
Description: The R12-31 monoclonal antibody reacts with mouse RANK (Receptor activator of NF-kappaB), a recently cloned member of the TNFR superfamily with no significant homology to other members of this family. RANK ligand (RANKL/TRANCE/ OPGL) binds to RANK on dendritic cells, upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in dendritic cell survival. The cytoplasmic domain of RANK interacts with TRAF2, TRAF5 and TRAF6. Overexpression of RANK activates NF-kappaB and c-Jun-terminal kinase (JNK) pathways. Recent studies have shown that RANK interaction with TRAF6 activates NF-kappaB, whereas JNK activation is mediated through binding of RANK to TRAF2.
Applications Reported: The R12-31 antibody has been reported for use in flow cytometric analysis.
Applications Tested: The R12-31 antibody has been tested by flow cytometric analysis of mouse RANK transfected cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
RANK is a member of the tumor necrosis factor receptor (TNFR) family. The full length mouse RANK cDNA encodes a type I transmembrane protein with an extracellular and a cytoplasmic domain. The extracellular domain contains two potential N-linked glycosylation sites. RANK shares significant amino acid homology with other members of the TNFR family in its extracellular four cysteine-rich repeats. Human and murine RANK share 81% amino acid identity in their extracellular domains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD265; ODFR; Osteoclast differentiation factor receptor; receptor activator of NF-kappaB; Receptor activator of NF-KB; Tumor necrosis factor receptor superfamily member 11A
Gene Aliases: Ly109; mRANK; ODFR; OFE; Rank; Tnfrsf11a; TRANCE-R
UniProt ID: (Mouse) O35305
Entrez Gene ID: (Mouse) 21934
ossification
adaptive immune response
multicellular organism development
programmed cell death
osteoclast differentiation
response to lipopolysaccharide
tumor necrosis factor-mediated signaling pathway
response to cytokine
response to tumor necrosis factor
positive regulation of JUN kinase activity
lymph node development
positive regulation of sequence-specific DNA binding transcription factor activity
positive regulation of NF-kappaB transcription factor activity
circadian temperature homeostasis
mammary gland alveolus development
response to interleukin-1
positive regulation of fever generation by positive regulation of prostaglandin secretion
TNFSF11-mediated signaling pathway
positive regulation of ERK1 and ERK2 cascade via TNFSF11-mediated signaling
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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