Invitrogen

CD271 (NGF Receptor) Monoclonal Antibody (ME20.4), Super Bright™ 600, eBioscience™

2 Published Figures
3 References
View all (99) NGFR (CD271) antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

63-9400-42

Applications
Tested Dilution
Publications

Immunohistochemistry (IHC)

-
View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL (0.25 µg)/test
View 2 publications 2 publications
Product Specifications

Species Reactivity

Pig, Rabbit, Rat, Dog, Human, Mouse, Non-human primate, Sheep

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Super Bright™ 600, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

ME20.4

Conjugate

Super Bright™ 600 Super Bright™ 600 Super Bright™ 600

Excitation/Emission Max

414/601 nm View spectra spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2762622

Product Specific Information

Description: The monoclonal antibody ME20.4 recognizes CD271, a 75 kDa, type I transmembrane protein that belongs to the low-affinity neurotrophin receptor and tumor necrosis factor receptor superfamily. CD271 is expressed by neurons, Schwann cells, melanocytes, mesenchymal stem/stromal cells, neural crest stem cells, astrocytes, oligodendrocytes, and a subset of lymphoid cells including follicular dendritic cells. CD271 binds to the ligands NGF, BDNF, NTF3, and NTF4. CD271 contains a death domain which induces apoptosis and regulates cell growth and proliferation through interactions with TRAF2, TRAF4, TRAF6, PTPN13, and RANBP9.

The ME20.4 monoclonal antibody recognizes human, non-human primate, mouse, rat, sheep, rabbit, cat, dog, and pig CD271.

Applications Reported: This ME20.4 antibody has been reported for use in flow cytometric analysis, western blotting, and microscopy.

Applications Tested: This ME20.4 antibody has been pre-titrated and tested by flow cytometric analysis of stimulated HEK-293T cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 405 nm; Emission: 600 nm; Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

Neurotrophic factors control the survival, differentiation and maintenance of neurons in the peripheral and central nervous systems, and of other neural crest-derived cell types. Developing sympathetic neurons are absolutely dependent upon nerve growth factor (NGF) during the period of target competition in vivo. Antibodies reacting specifically with NGFR p75 are useful tools in the detection and characterization of NGFR p75, to enhance our understanding of a wide range of phenomena in the development, plasticity and repair of the nervous system.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Bioinformatics

Protein Aliases: CD271; CD271 antigen; Gp80-LNGFR; low affinity nerve growth factor receptor; Low affinity neurotrophin receptor p75NTR; Low-affinity nerve growth factor receptor; Low-affinity nerve growth factor receptor p75NGFR; Low-affinity nerve growth factor receptor p75NGR; nerve growth factor receptor (TNFR superfamily, member 16); nerve growth factor receptor, fast; NGF receptor; p-75; p75 ICD; p75 neurotrophin receptor; p75 ntr; TNFR superfamily, member 16; TNR16; Tumor necrosis factor receptor superfamily member 16

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Gene Aliases: CD271; Gp80-LNGFR; LNGFR; NGFR; p75; p75(NTR); p75NGFR; p75NTR; RNNGFRR; TNFRSF16

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UniProt ID: (Human) P08138, (Rat) P07174, (Mouse) Q9Z0W1

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Entrez Gene ID: (Sheep) 101119064, (Dog) 491071, (Human) 4804, (Rat) 24596, (Mouse) 18053

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Function(s)
beta-amyloid binding signal transducer activity receptor activity transmembrane signaling receptor activity tumor necrosis factor-activated receptor activity neurotrophin TRKA receptor binding protein binding Rab GTPase binding ubiquitin protein ligase binding nerve growth factor binding preprotein binding death receptor activity neurotrophin binding
Process(es)
intracellular protein transport apoptotic process signal transduction axon guidance central nervous system development response to wounding regulation of gene expression positive regulation of cell death negative regulation of neuron projection development programmed cell death detection of temperature stimulus sensory perception of pain nerve development hair follicle morphogenesis positive regulation of myelination positive regulation of synaptic transmission, cholinergic response to lipopolysaccharide circadian regulation of gene expression cellular response to oxidative stress positive regulation of Rho protein signal transduction skeletal muscle cell differentiation negative regulation of fibroblast growth factor receptor signaling pathway positive regulation of odontogenesis of dentin-containing tooth glucose homeostasis positive regulation of apoptotic process positive regulation of MAPK cascade negative regulation of neuron apoptotic process skin development positive regulation of fibroblast proliferation negative regulation of hair follicle development positive regulation of protein kinase B signaling negative regulation of mitochondrial depolarization positive regulation of synaptic transmission, glutamatergic negative regulation of cell death positive regulation of neuron death regulation of reactive oxygen species metabolic process positive regulation of apoptotic signaling pathway regulation of glucose import in response to insulin stimulus activation of cysteine-type endopeptidase activity involved in apoptotic process inflammatory response immune response membrane protein intracellular domain proteolysis tumor necrosis factor-mediated signaling pathway regulation of cell proliferation regulation of apoptotic process negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process regulation of cysteine-type endopeptidase activity involved in apoptotic process neurotrophin TRK receptor signaling pathway negative regulation of axonogenesis positive regulation of axonogenesis apoptotic signaling pathway multicellular organism development nervous system development cell differentiation rhythmic process
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