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Invitrogen
CD275 (B7-H2) Chimeric Recombinant Rabbit Monoclonal Antibody (HK5.3)
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CD275 (B7-H2) Chimeric Antibody (MA5-48098) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of CD275 (B7-H2) in mouse splenocytes using CD275 (B7-H2) Chimeric Monoclonal Antibody (Product # MA5-48098). Paraformaldehyde-fixed cells were immobilized on cover slips and stained with CD275 (B7-H2) Chimeric Monoclonal Antibody at 10 µg/mL for 1h followed by Alexa Fluor488 secondary antibody (1 µg/mL), showing weak membrane staining in a subset of cells. Th... View More
Product Details
MA5-48098
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG, kappa
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
HK5.3
Immunogen
This antibody was raised by immunizing SD rat with mouse B7h-transfected L cells and fusing immune splenocytes with P3U1 myeloma cells and screened for binding to mouse B7h-transfected NRK cells.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.02% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3074541
Product Specific Information
Specificity: This antibody specifically binds to mouse ICOSL and competes with binding of ICOS.
Target Information
Inducible co-stimulator ligand (ICOSL) is a specific ligand on antigen-presenting cells and cells of the peripheral tissue that binds to the inducible co-stimulator receptor (ICOS). ICOS belongs to the CD28/CD152 receptor family that regulates T-cell activation and function. ICOSL is expressed on monocytes, dendritic cells and B cells and can be induced by inflammatory stimuli in peripheral tissue. Binding to ICOSL delivers a co-stimulatory signal for T cell proliferation and cytokine secretion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B7 homolog 2; B7-H2; B7-like protein Gl50; B7-related protein 1; B7RP-1; CD275; ICOS ligand; LICOS
Gene Aliases: AU044799; B7-H2; B7h; B7h2; B7RP-1; B7rp1; BG071784; GI50; GL50; GL50-B; ICOS-L; Icosl; Icoslg; LICOS; Ly115l; mKIAA0653
UniProt ID: (Mouse) Q9JHJ8
Entrez Gene ID: (Mouse) 50723
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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