Invitrogen

CD279 (PD-1) Monoclonal Antibody (J43), PerCP-eFluor™ 710, eBioscience™

40 Published Figures

41 References

View all (273) PD-1 (CD279) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (40)

CD279 (PD-1) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 41

CD279 (PD-1) Antibody (46-9985-82) in Flow

Staining of 3-day Anti-Mouse CD3 and Anti-Mouse CD28 Functional Grade Purified (Product # 16-0031-82 and Product # 16-0281-82)-stimulated C57Bl/6 splenocytes with 0.06 µg of Armenian Hamster IgG Isotype Control PerCP-eFluor® 710 (Product # 46-4888-82) (blue histogram) or 0.06 µg of Anti-Mouse CD279 (PD-1) PerCP-eFluor® 710 (purple histogram). Total viable cells, as determined by Fixable Viability Dye eFluor® 450 (Product # 65-0863-14), were used for analysis.

Product Image Gallery

Product Details

46-9985-82

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.125 µg/test

View 38 publications 38 publications

Functional Assay (Functional)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), PerCP-eFluor™ 710, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

J43

Conjugate

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

Excitation/Emission Max

482/708 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_11150055

Product Specific Information

Description: The J43 monoclonal antibody reacts with mouse PD-1 (programmed death-1), a 55 kDa member of the Ig superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease in mice. PD-1 is expressed mainly on activated T and B lymphocytes. Two novel B7 Family members have been identified as PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). Evidence reported to date suggests overlapping functions for these ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. It is reported that J43 inhibits the binding of mouse PD-L1-Ig and mouse PD-L2-Ig to PD-1/BHK transfected cells. When administrated in vivo, both intact and Fab of J43 are reported to enhance contact hypersensitivity and exacerbate acute GVHD similar to transfer of PD-1-deficient cells. Injection of J43 also exacerbates EAE and NOD diabetes as do specific antibodies to mouse PD-L1 and PD-L2.

Applications Reported: This J43 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This J43 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.

Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

A single-amino acid substitution in the adaptor LAT accelerates TCR proofreading kinetics and alters T-cell selection, maintenance and function.

Nature immunology

Lo WL,Kuhlmann M,Rizzuto G,Ekiz HA,Kolawole EM,Revelo MP,Andargachew R,Li Z,Tsai YL,Marson A,Evavold BD,Zehn D,Weiss A

46-9985-82 was used in Immunocytochemistry to reveal the importance of kinetic proofreading in balancing tolerance and immunity.

Sat Apr 01 00:00:00 UTC 2023

CD153/CD30 signaling promotes age-dependent tertiary lymphoid tissue expansion and kidney injury.

The Journal of clinical investigation

Sato Y,Oguchi A,Fukushima Y,Masuda K,Toriu N,Taniguchi K,Yoshikawa T,Cui X,Kondo M,Hosoi T,Komidori S,Shimizu Y,Fujita H,Jiang L,Kong Y,Yamanashi T,Seita J,Yamamoto T,Toyokuni S,Hamazaki Y,Hattori M,Yoshikai Y,Boor P,Floege J,Kawamoto H,Murakawa Y,Minato N,Yanagita M

Published figure using CD279 (PD-1) monoclonal antibody (Product # 46-9985-82) in Flow Cytometry

Tue Jan 18 00:00:00 UTC 2022

Ultrasound combined with nanobubbles promotes systemic anticancer immunity and augments anti-PD1 efficacy.

Journal for immunotherapy of cancer

Hu J,He J,Wang Y,Zhao Y,Fang K,Dong Y,Chen Y,Zhang Y,Zhang C,Wang H,Tan J,Wang J,Zi R,Liu C,Liang H,Guo Y,Ou J

Published figure using CD279 (PD-1) monoclonal antibody (Product # 46-9985-82) in Flow Cytometry

Tue Mar 01 00:00:00 UTC 2022

Mobilizing phospholipids on tumor plasma membrane implicates phosphatidylserine externalization blockade for cancer immunotherapy.

Cell reports

Wang W,Wu S,Cen Z,Zhang Y,Chen Y,Huang Y,Cillo AR,Prokopec JS,Quarato G,Vignali DAA,Stewart-Ornstein J,Li S,Lu B,Gong YN

Published figure using CD279 (PD-1) monoclonal antibody (Product # 46-9985-82) in Flow Cytometry

Tue Nov 01 00:00:00 UTC 2022

CD4⁺ CTLs Act as a Key Effector Population for Allograft Rejection of MSCs in a Donor MHC-II Dependent Manner in Injured Liver.

Aging and disease

Shen S,Li Y,Jin M,Fan D,Pan R,Lin A,Chen Y,Xiang L,Zhao RC,Shao J

Published figure using CD279 (PD-1) monoclonal antibody (Product # 46-9985-82) in Flow Cytometry

Thu Dec 01 00:00:00 UTC 2022

PRDM1/BLIMP1 induces cancer immune evasion by modulating the USP22-SPI1-PD-L1 axis in hepatocellular carcinoma cells.

Nature communications

Li Q,Zhang L,You W,Xu J,Dai J,Hua D,Zhang R,Yao F,Zhou S,Huang W,Dai Y,Zhang Y,Baheti T,Qian X,Pu L,Xu J,Xia Y,Zhang C,Tang J,Wang X

Published figure using CD279 (PD-1) monoclonal antibody (Product # 46-9985-82) in Flow Cytometry

Mon Dec 12 00:00:00 UTC 2022

Nucleolin Therapeutic Targeting Decreases Pancreatic Cancer Immunosuppression.

Cancers

Ponzo M,Debesset A,Cossutta M,Chalabi-Dchar M,Houppe C,Pilon C,Nicolas-Boluda A,Meunier S,Raineri F,Thiolat A,Nicolle R,Maione F,Brundu S,Cojocaru CF,Bouvet P,Bousquet C,Gazeau F,Tournigand C,Courty J,Giraudo E,Cohen JL,Cascone I

46-9985-82 was used in Flow cytometry/Cell sorting to show that nucleolin (NCL) inhibition normalizes tumour vessels and impairs PDAC growth.

Wed Aug 31 00:00:00 UTC 2022

NF-κB-inducing kinase maintains T cell metabolic fitness in antitumor immunity.

Nature immunology

Gu M,Zhou X,Sohn JH,Zhu L,Jie Z,Yang JY,Zheng X,Xie X,Yang J,Shi Y,Brightbill HD,Kim JB,Wang J,Cheng X,Sun SC

Published figure using CD279 (PD-1) monoclonal antibody (Product # 46-9985-82) in Flow Cytometry

Mon Feb 01 00:00:00 UTC 2021

Graded RhoA GTPase Expression in Treg Cells Distinguishes Tumor Immunity From Autoimmunity.

Frontiers in immunology

Kalim KW,Yang JQ,Modur V,Nguyen P,Li Y,Zheng Y,Guo F

Published figure using CD279 (PD-1) monoclonal antibody (Product # 46-9985-82) in Flow Cytometry

Tue Jan 11 00:00:00 UTC 2022

Stearoyl-CoA Desaturase-Mediated Monounsaturated Fatty Acid Availability Supports Humoral Immunity.

Cell reports

Zhou X,Zhu X,Li C,Li Y,Ye Z,Shapiro VS,Copland JA,Hitosugi T,Bernlohr DA,Sun J,Zeng H

Published figure using CD279 (PD-1) monoclonal antibody (Product # 46-9985-82) in Flow Cytometry

Tue Jan 05 00:00:00 UTC 2021

Ketogenic diet and ketone bodies enhance the anticancer effects of PD-1 blockade.

JCI insight

Ferrere G,Tidjani Alou M,Liu P,Goubet AG,Fidelle M,Kepp O,Durand S,Iebba V,Fluckiger A,Daillère R,Thelemaque C,Grajeda-Iglesias C,Alves Costa Silva C,Aprahamian F,Lefevre D,Zhao L,Ryffel B,Colomba E,Arnedos M,Drubay D,Rauber C,Raoult D,Asnicar F,Spector T,Segata N,Derosa L,Kroemer G,Zitvogel L

Published figure using CD279 (PD-1) monoclonal antibody (Product # 46-9985-82) in Flow Cytometry

Mon Jan 25 00:00:00 UTC 2021

Histone deacetylase 3 represses cholesterol efflux during CD4⁺ T-cell activation.

eLife

Wilfahrt D,Philips RL,Lama J,Kizerwetter M,Shapiro MJ,McCue SA,Kennedy MM,Rajcula MJ,Zeng H,Shapiro VS

Published figure using CD279 (PD-1) monoclonal antibody (Product # 46-9985-82) in Flow Cytometry

Thu Dec 02 00:00:00 UTC 2021

PTENα functions as an immune suppressor and promotes immune resistance in PTEN-mutant cancer.

Nature communications

Sun Y,Lu D,Yin Y,Song J,Liu Y,Hao W,Qi F,Zhang G,Zhang X,Liu L,Lin Z,Liang H,Zhao X,Jin Y,Yin Y

46-9985-82 was used in Flow Cytometry to propose that PTENα protects tumor from T cell killing and thus PTENα is a potential target in antitumor immunotherapy.

Thu Aug 26 00:00:00 UTC 2021

Activation of regulatory T cells triggers specific changes in glycosylation associated with Siglec-1-dependent inflammatory responses.

Wellcome open research

Wu G,Murugesan G,Nagala M,McCraw A,Haslam SM,Dell A,Crocker PR

Published figure using CD279 (PD-1) monoclonal antibody (Product # 46-9985-82) in Flow Cytometry

Thu Aug 22 00:00:00 UTC 2024

CD122-targeted interleukin-2 and αPD-L1 treat bladder cancer and melanoma via distinct mechanisms, including CD122-driven natural killer cell maturation.

Oncoimmunology

Reyes RM,Zhang C,Deng Y,Ji N,Mukherjee N,Padron AS,Clark CA,Svatek RS,Curiel TJ

46-9985-82 was used in Flow cytometry/Cell sorting to support that αPD-L1 and IL-2c mediate nonredundant, immune microenvironment-specific treatment mechanisms involving CD8+ T and NK cells in primary and metastatic BC and melanoma.

Mon Jan 03 00:00:00 UTC 2022

IL4I1 Is a Metabolic Immune Checkpoint that Activates the AHR and Promotes Tumor Progression.

Cell

Sadik A,Somarribas Patterson LF,Öztürk S,Mohapatra SR,Panitz V,Secker PF,Pfänder P,Loth S,Salem H,Prentzell MT,Berdel B,Iskar M,Faessler E,Reuter F,Kirst I,Kalter V,Foerster KI,Jäger E,Guevara CR,Sobeh M,Hielscher T,Poschet G,Reinhardt A,Hassel JC,Zapatka M,Hahn U,von Deimling A,Hopf C,Schlichting R,Escher BI,Burhenne J,Haefeli WE,Ishaque N,Böhme A,Schäuble S,Thedieck K,Trump S,Seiffert M,Opitz CA

46-9985 was used in Flow cytometry/Cell sorting to show that IL4I1 blockade opens new avenues for cancer therapy.

Thu Sep 03 00:00:00 UTC 2020

Transplantation tolerance modifies donor-specific B cell fate to suppress de novo alloreactive B cells.

The Journal of clinical investigation

Khiew SH,Jain D,Chen J,Yang J,Yin D,Young JS,Dent A,Sciammas R,Alegre ML,Chong AS

46-9985 was used in Flow cytometry/Cell sorting to showed that alloreactive B cells were not deleted but rapidly lost their ability to differentiate into germinal center B cells and secrete donor-specific antibodies., by using a murine model of heart tolerance.

Wed Jul 01 00:00:00 UTC 2020

Serum HBsAg clearance has minimal impact on CD8+ T cell responses in mouse models of HBV infection.

The Journal of experimental medicine

Fumagalli V,Di Lucia P,Venzin V,Bono EB,Jordan R,Frey CR,Delaney W,Chisari FV,Guidotti LG,Iannacone M

Published figure using CD279 (PD-1) monoclonal antibody (Product # 46-9985-82) in Flow Cytometry

Mon Nov 02 00:00:00 UTC 2020

B cells are sufficient to prime the dominant CD4+ Tfh response to Plasmodium infection.

The Journal of experimental medicine

Arroyo EN,Pepper M

Published figure using CD279 (PD-1) monoclonal antibody (Product # 46-9985-82) in Flow Cytometry

Mon Feb 03 00:00:00 UTC 2020

Inhibition of the Wnt/β-catenin pathway enhances antitumor immunity in ovarian cancer.

Therapeutic advances in medical oncology

Doo DW,Meza-Perez S,Londoño AI,Goldsberry WN,Katre AA,Boone JD,Moore DJ,Hudson CT,Betella I,McCaw TR,Gangrade A,Bao R,Luke JJ,Yang ES,Birrer MJ,Starenki D,Cooper SJ,Buchsbaum DJ,Norian LA,Randall TD,Arend RC

Published figure using CD279 (PD-1) monoclonal antibody (Product # 46-9985-82) in Flow Cytometry

Thu Apr 14 00:00:00 UTC 2022

Gene silencing of indoleamine 2,3-dioxygenase 1 inhibits lung cancer growth by suppressing T-cell exhaustion.

Oncology letters

Shang K,Wang Z,Hu Y,Huang Y,Yuan K,Yu Y

Published figure using CD279 (PD-1) monoclonal antibody (Product # 46-9985-82) in Flow Cytometry

Mon Jun 01 00:00:00 UTC 2020

The Selenoprotein MsrB1 Instructs Dendritic Cells to Induce T-Helper 1 Immune Responses.

Antioxidants (Basel, Switzerland)

Lee HJ,Park JS,Yoo HJ,Lee HM,Lee BC,Kim JH

Published figure using CD279 (PD-1) monoclonal antibody (Product # 46-9985-82) in Flow Cytometry

Tue Oct 20 00:00:00 UTC 2020

Lysosomal Acid Lipase Is Required for Donor T Cells to Induce Graft-versus-Host Disease.

Cell reports

Nguyen HD,Ticer T,Bastian D,Kuril S,Li H,Du H,Yan C,Yu XZ

Published figure using CD279 (PD-1) monoclonal antibody (Product # 46-9985-82) in Flow Cytometry

Tue Oct 27 00:00:00 UTC 2020

5-Azacytidine Potentiates Anti-tumor Immunity in a Model of Pancreatic Ductal Adenocarcinoma.

Frontiers in immunology

Ebelt ND,Zuniga E,Johnson BL,Diamond DJ,Manuel ER

Published figure using CD279 (PD-1) monoclonal antibody (Product # 46-9985-82) in Flow Cytometry

Fri Mar 26 00:00:00 UTC 2021

Endogenous retroviral proteins provide an immunodominant but not requisite antigen in a murine immunotherapy tumor model.

Oncoimmunology

Ye X,Waite JC,Dhanik A,Gupta N,Zhong M,Adler C,Malahias E,Ni M,Wei Y,Gurer C,Zhang W,Macdonald LE,Murphy AJ,Sleeman MA,Skokos D

46-9985 was used in Flow cytometry/Cell sorting to reveal that endogenous retroviral antigens expressed by tumour cells may represent an important and underappreciated category of tumour antigens that could be readily targeted in the clinic.

Wed May 13 00:00:00 UTC 2020

Endogenous retroviral proteins provide an immunodominant but not requisite antigen in a murine immunotherapy tumor model.

Oncoimmunology

Ye X,Waite JC,Dhanik A,Gupta N,Zhong M,Adler C,Malahias E,Ni M,Wei Y,Gurer C,Zhang W,Macdonald LE,Murphy AJ,Sleeman MA,Skokos D

Wed May 13 00:00:00 UTC 2020

Diverse CD8 T Cell Responses to Viral Infection Revealed by the Collaborative Cross.

Cell reports

Martin MD,Sompallae R,Winborn CS,Harty JT,Badovinac VP

46-9985 was used in Flow cytometry/Cell sorting to advance the utility of the Collaborative Cross (CC) as a tool to analyze the immune response to viral infection.

Tue Apr 14 00:00:00 UTC 2020

Repurposing the selective estrogen receptor modulator bazedoxifene to suppress gastrointestinal cancer growth.

EMBO molecular medicine

Thilakasiri P,Huynh J,Poh AR,Tan CW,Nero TL,Tran K,Parslow AC,Afshar-Sterle S,Baloyan D,Hannan NJ,Buchert M,Scott AM,Griffin MD,Hollande F,Parker MW,Putoczki TL,Ernst M,Chand AL

46-9985-82 was used in Flow Cytometry to establish the in vivo utility of bazedoxifene, a steroid analog clinically approved for the treatment of osteoporosis, to suppress gp130-dependent tumor growth of the gastrointestinal epithelium.

Mon Apr 01 00:00:00 UTC 2019

Combination PD-1 and PD-L1 Blockade Promotes Durable Neoantigen-Specific T Cell-Mediated Immunity in Pancreatic Ductal Adenocarcinoma.

Cell reports

Burrack AL,Spartz EJ,Raynor JF,Wang I,Olson M,Stromnes IM

Published figure using CD279 (PD-1) monoclonal antibody (Product # 46-9985-82) in Flow Cytometry

Tue Aug 20 00:00:00 UTC 2019

CXCR5⁺PD-1⁺ follicular helper CD8 T cells control B cell tolerance.

Nature communications

Chen Y,Yu M,Zheng Y,Fu G,Xin G,Zhu W,Luo L,Burns R,Li QZ,Dent AL,Zhu N,Cui W,Malherbe L,Wen R,Wang D

46-9985 was used in Flow cytometry/Cell sorting to highlight the diversity of follicular T cell subsets that contribute to the breakdown of B cell tolerance.

Fri Sep 27 00:00:00 UTC 2019

Initiation of Antiviral B Cell Immunity Relies on Innate Signals from Spatially Positioned NKT Cells.

Cell

Gaya M,Barral P,Burbage M,Aggarwal S,Montaner B,Warren Navia A,Aid M,Tsui C,Maldonado P,Nair U,Ghneim K,Fallon PG,Sekaly RP,Barouch DH,Shalek AK,Bruckbauer A,Strid J,Batista FD

Published figure using CD279 (PD-1) monoclonal antibody (Product # 46-9985-82) in Flow Cytometry

Thu Jan 25 00:00:00 UTC 2018

Galectin-8 Favors the Presentation of Surface-Tethered Antigens by Stabilizing the B Cell Immune Synapse.

Cell reports

Obino D,Fetler L,Soza A,Malbec O,Saez JJ,Labarca M,Oyanadel C,Del Valle Batalla F,Goles N,Chikina A,Lankar D,Segovia-Miranda F,Garcia C,Léger T,Gonzalez A,Espéli M,Lennon-Duménil AM,Yuseff MI

46-9985 was used in Flow cytometry/Cell sorting to examine the mechanism by which Galectin-8 favours the presentation of surface-tethered antigens.

Tue Dec 11 00:00:00 UTC 2018

Oncolytic Adenovirus and Tumor-Targeting Immune Modulatory Therapy Improve Autologous Cancer Vaccination.

Cancer research

Jiang H,Rivera-Molina Y,Gomez-Manzano C,Clise-Dwyer K,Bover L,Vence LM,Yuan Y,Lang FF,Toniatti C,Hossain MB,Fueyo J

Published figure using CD279 (PD-1) monoclonal antibody (Product # 46-9985-82) in Flow Cytometry

Sat Jul 15 00:00:00 UTC 2017

Therapeutic efficacy of combined vaccination against tumor pericyte-associated antigens DLK1 and DLK2 in mice.

Oncoimmunology

Fabian KP,Chi-Sabins N,Taylor JL,Fecek R,Weinstein A,Storkus WJ

Published figure using CD279 (PD-1) monoclonal antibody (Product # 46-9985-82) in Flow Cytometry

Tue Jan 12 00:00:00 UTC 2021

Intratumoral modulation of the inducible co-stimulator ICOS by recombinant oncolytic virus promotes systemic anti-tumour immunity.

Nature communications

Zamarin D,Holmgaard RB,Ricca J,Plitt T,Palese P,Sharma P,Merghoub T,Wolchok JD,Allison JP

Published figure using CD279 (PD-1) monoclonal antibody (Product # 46-9985-82) in Flow Cytometry

Mon Feb 13 00:00:00 UTC 2017

Cblb-deficient T cells are less susceptible to PD-L1-mediated inhibition.

Oncotarget

Peer S,Baier G,Gruber T

Published figure using CD279 (PD-1) monoclonal antibody (Product # 46-9985-82) in Flow Cytometry

Tue Jun 27 00:00:00 UTC 2017

Growth and metastasis of lung adenocarcinoma is potentiated by BMP4-mediated immunosuppression.

Oncoimmunology

Chen L,Yi X,Goswami S,Ahn YH,Roybal JD,Yang Y,Diao L,Peng D,Peng D,Fradette JJ,Wang J,Byers LA,Kurie JM,Ullrich SE,Qin FX,Gibbons DL

Published figure using CD279 (PD-1) monoclonal antibody (Product # 46-9985-82) in Flow Cytometry

Tue Jan 12 00:00:00 UTC 2021

The stress kinase GCN2 does not mediate suppression of antitumor T cell responses by tryptophan catabolism in experimental melanomas.

Oncoimmunology

Sonner JK,Deumelandt K,Ott M,Thomé CM,Rauschenbach KJ,Schulz S,Munteanu B,Mohapatra S,Adam I,Hofer AC,Feuerer M,Opitz CA,Hopf C,Wick W,Platten M

Published figure using CD279 (PD-1) monoclonal antibody (Product # 46-9985-82) in Flow Cytometry

Tue Jan 12 00:00:00 UTC 2021

Metastasis is regulated via microRNA-200/ZEB1 axis control of tumour cell PD-L1 expression and intratumoral immunosuppression.

Nature communications

Chen L,Gibbons DL,Goswami S,Cortez MA,Ahn YH,Byers LA,Zhang X,Yi X,Dwyer D,Lin W,Diao L,Wang J,Roybal J,Patel M,Ungewiss C,Peng D,Antonia S,Mediavilla-Varela M,Robertson G,Suraokar M,Welsh JW,Erez B,Wistuba II,Chen L,Peng D,Wang S,Ullrich SE,Heymach JV,Kurie JM,Qin FX

Published figure using CD279 (PD-1) monoclonal antibody (Product # 46-9985-82) in Flow Cytometry

Tue Oct 28 00:00:00 UTC 2014

The programmed death-1 (PD-1) pathway regulates autoimmune diabetes in nonobese diabetic (NOD) mice.

The Journal of experimental medicine

Ansari MJ,Salama AD,Chitnis T,Smith RN,Yagita H,Akiba H,Yamazaki T,Azuma M,Iwai H,Khoury SJ,Auchincloss H,Sayegh MH

Mon Jul 07 00:00:00 UTC 2003

The programmed death-1 (PD-1) pathway regulates autoimmune diabetes in nonobese diabetic (NOD) mice.

The Journal of experimental medicine

Ansari MJ,Salama AD,Chitnis T,Smith RN,Yagita H,Akiba H,Yamazaki T,Azuma M,Iwai H,Khoury SJ,Auchincloss H,Sayegh MH

Mon Jul 07 00:00:00 UTC 2003

The programmed death-1 (PD-1) pathway regulates autoimmune diabetes in nonobese diabetic (NOD) mice.

The Journal of experimental medicine

Ansari MJ,Salama AD,Chitnis T,Smith RN,Yagita H,Akiba H,Yamazaki T,Azuma M,Iwai H,Khoury SJ,Auchincloss H,Sayegh MH

Mon Jul 07 00:00:00 UTC 2003

Bioinformatics

Protein Aliases: CD279; hPD1; mPD-1; Programmed cell death protein 1; Protein PD-1; Protein PD1; sCD279; soluble CD279

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Gene Aliases: Ly101; PD-1; Pd1; Pdc1; Pdcd1

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UniProt ID: (Mouse) Q02242

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Entrez Gene ID: (Mouse) 18566

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Function(s)

protein binding

Process(es)

immune system process negative regulation of tolerance induction apoptotic process positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of T cell apoptotic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD279 (PD-1) Monoclonal Antibody (J43), PerCP-eFluor™ 710, eBioscience™

CD279 (PD-1) Antibody (46-9985-82) in Flow

Product Details

46-9985-82

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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