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Invitrogen
CD28 Monoclonal Antibody (5B8), APC, eBioscience™
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FIGURE: 1 / 2
CD28 Antibody (17-0282-42) in Flow
Canine and human PBMCs were stained with CD28 APC antibody (Product # 17-0282-42) for 30 min on ice with indicated concentrations. After stanning, cells were washed by PBS with 5% heat inactivated calf serum + 5mM EDTA. Data were acquired immediately after resuspending cells in PBS with 1% formaldehyde. Total viable lymphocytes were gated for analysis. Data courtesy of Thermo Scientific KOL Program.
Product Details
17-0282-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog
Published species
Dog
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
5B8
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2573140
Product Specific Information
Description: The 5B8 monoclonal antibody reacts with canine CD28, which is expressed by thymocytes, a majority of T cells, and a subset of NK cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent costimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells.
The 5B8 antibody has been reported to have agonist activity. The 5B8 and 1C6 antibodies recognize different epitopes of canine CD28.
Applications Reported: This 5B8 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 5B8 antibody has been pre-titrated and tested by flow cytometric analysis of normal canine peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. CD28 is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes. Moreover, CD28 is the critical T cell costimulatory receptor that provides the cell the important second activation signal by binding CD80 and CD86 which are expressed by antigen presenting cells. In addition to its co-stimulation role, CD28 functions by preventing T cells from entering an anergic-hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells. Diseases associated with CD28 dysfunction include mycosis fungiodes and Sezary's Disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: CD28 antigen (Tp44); cell surface protein; costimulatory molecule B7 receptor CD28; MGC138290; sCD28; soluble CD28; T-cell costimulatory molecule CD28; T44
Entrez Gene ID: (Dog) 403646
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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