Invitrogen

CD282 (TLR2) Monoclonal Antibody (6C2), PE, eBioscience™

4 Published Figures

21 References

View all (75) TLR2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (4)

CD282 (TLR2) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 5

CD282 (TLR2) Antibody (12-9021-82) in Flow

Staining of C57BL/6 splenocytes with Anti-Mouse CD11b FITC (Product # 11-0112-41) and 0.5 µg of Rat IgG2b kappa Isotype Control PE (Product # 12-4031-82) (left) or 0.5 µg of Anti-Mouse CD282 (TLR2) PE (right). Samples were pre-incubated with Anti-Mouse CD16/CD32 Purified (Product # 14-0161-82). Total viable cells were used for analysis.

Product Image Gallery

Product Details

12-9021-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1 µg/test

View 21 publications 21 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

6C2

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_466230

Product Specific Information

Description: The 6C2 monoclonal antibody reacts with mouse Toll-like receptor 2 (TLR2). mouse TLR2 is expressed by the myeloid lineage, including macrophage and dendritic cells in splenocytes and the RAW264.7 cell line. To date, at least twelve members of the Toll family have been identified in human and mouse. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation. TLR2 is expressed on the surface of cells and is responsible for distinguishing different pathogens.

Applications Reported: 6C2 has been reported for use in flow cytometric analysis. For optimal flow cytometry staining with this antibody, it is recommended that a directly conjugated 6C2 (FITC, PE, or Biotin) be used in combination with FcR blocking with anti-mouse CD16/CD32 (clone 93, Product # 14-0161).

Applications Tested: The 6C2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

TLR2 is a member of the Toll-like receptor (TLR) family which play a fundamental role in pathogen recognition and activation of innate immunity. TLR proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors such as Protein Kinase C (PKC) alpha/beta and NF-kappa-B. TLR2 can form heterodimers with either TLR1 or TLR6, and as a heterodimer, can recognize a variety of bacterial and mycoplasma lipoproteins respectively. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. The various TLRs exhibit different patterns of expression. TLR2 is expressed most abundantly in peripheral blood leukocytes, and mediates host response to Gram-positive bacteria and yeast via stimulation of NF-kappaB. TLR2 aids in the recognition of pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. Ten human homologs of TLRs (TLR1-10) have been described. TLR2 is expressed in human cells such as tonsils, microglia, lymph nodes, and appendices, activated B-cells in germinal centers. CD14+ monocytes express the highest level of TLR2 followed by CD15+ granulocytes, CD19+ B-cells, and CD3+ T-cells. The expression of TLR2 on different cell types are regulated by different immune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Glial cell line-derived neurotrophic factor increases matrix metallopeptidase 9 and 14 expression in microglia and promotes microglia-mediated glioma progression.

Journal of neuroscience research

Huang Y,Zhang B,Haneke H,Haage V,Lubas M,Yuan Y,Xia P,Motta E,Nanvuma C,Dzaye O,Hu F,Kettenmann H

Published figure using CD282 (TLR2) monoclonal antibody (Product # 12-9021-82) in Flow Cytometry

Thu Apr 01 00:00:00 UTC 2021

Genomic and epigenomic adaptation in SP-R210 (Myo18A) isoform-deficient macrophages.

Immunobiology

Yau E,Chen Y,Song C,Webb J,Carillo M,Kawasawa YI,Tang Z,Takahashi Y,Umstead TM,Dovat S,Chroneos ZC

12-9021-82 was used in Flow Cytometry to suggest that the inflammatory differences seen in SP-R210L-deficient macrophages are a result of transcriptional differences that are mediated by epigenetic changes brought about by differential expression of the SP-R210 isoforms.

Mon Nov 01 00:00:00 UTC 2021

Induction of Foxp3 and activation of Tregs by HSP gp96 for treatment of autoimmune diseases.

iScience

Xu Y,Liu E,Xie X,Wang J,Zheng H,Ju Y,Chen L,Li C,Zhou X,Li Z,Li X,Meng S

12-9021-82 was used in Flow cytometry/Cell sorting to show that gp96 immunization restricted antibody-induced and Th-induced autoimmune diseases by integrating Treg expansion and activation, indicating its potential clinical usefulness against autoimmune diseases.

Fri Dec 17 00:00:00 UTC 2021

Bacteroides fragilis polysaccharide A induces IL-10 secreting B and T cells that prevent viral encephalitis.

Nature communications

Ramakrishna C,Kujawski M,Chu H,Li L,Mazmanian SK,Cantin EM

12-9021 was used in Flow cytometry/Cell sorting to study polysaccharide A as an immunomodulatory symbiosis factor whcih coordinates anti-inflammatory responses during viral infections.

Tue May 14 00:00:00 UTC 2019

Bacteroides fragilis polysaccharide A induces IL-10 secreting B and T cells that prevent viral encephalitis.

Nature communications

Ramakrishna C,Kujawski M,Chu H,Li L,Mazmanian SK,Cantin EM

12-9021 was used in Flow cytometry/Cell sorting to study polysaccharide A as an immunomodulatory symbiosis factor whcih coordinates anti-inflammatory responses during viral infections.

Tue May 14 00:00:00 UTC 2019

Traditional Herbal Medicine-Derived Sulforaphene LFS-01 Reverses Colitis in Mice by Selectively Altering the Gut Microbiota and Promoting Intestinal Gamma-Delta T Cells.

Frontiers in pharmacology

Li M,Gao J,Tang Y,Liu M,Wu S,Qu K,Long X,Li H,Liu M,Liu Y,Yuan J,Mao L,Liu Y,Zheng X,Wang E,Wang J,Yang Y

Published figure using CD282 (TLR2) monoclonal antibody (Product # 12-9021-82) in Flow Cytometry

Wed Sep 30 00:00:00 UTC 2020

TLR2^-/- Mice Display Increased Clearance of Dermatophyte Trichophyton mentagrophytes in the Setting of Hyperglycemia.

Frontiers in cellular and infection microbiology

Almeida DF,Fraga-Silva TF,Santos AR,Finato AC,Marchetti CM,Golim MA,Lara VS,Arruda MS,Venturini J

12-9021 was used in Flow cytometry/Cell sorting to demonstrate that TLR2 diminishes the development of adaptive immune responses during experimental deep dermatophytosis and, in a diabetic scenario, acts to intensify a non-protective inflammatory response.

Mon Sep 18 00:00:00 UTC 2017

Immunosuppression, peripheral inflammation and invasive infection from endogenous gut microbiota activate retinal microglia in mouse models.

Microbiology and immunology

Maneu V,Noailles A,Gómez-Vicente V,Carpena N,Cuenca N,Gil ML,Gozalbo D

12-9021 was used in Flow cytometry/Cell sorting to determine whether non-ocular conditions could lead to the activation of retinal microglia.

Thu Sep 01 00:00:00 UTC 2016

Porphyromonas gingivalis gingipains selectively reduce CD14 expression, leading to macrophage hyporesponsiveness to bacterial infection.

Journal of innate immunity

Wilensky A,Tzach-Nahman R,Potempa J,Shapira L,Nussbaum G

12-9021 was used in Flow cytometry/Cell sorting to study how porphyromonas gingivalis gingipains can reduce CD14 expression without affecting expression of the bacterial-sensing TLRs.

Mon Dec 21 00:00:00 UTC 2015

CX3CR1-dependent renal macrophage survival promotes Candida control and host survival.

The Journal of clinical investigation

Lionakis MS,Swamydas M,Fischer BG,Plantinga TS,Johnson MD,Jaeger M,Green NM,Masedunskas A,Weigert R,Mikelis C,Wan W,Lee CC,Lim JK,Rivollier A,Yang JC,Laird GM,Wheeler RT,Alexander BD,Perfect JR,Gao JL,Kullberg BJ,Netea MG,Murphy PM

12-9021 was used in Flow cytometry/Cell sorting to elucidate the roles of innate immune cells in the pathogenesis of C. albicans.

Sun Dec 01 00:00:00 UTC 2013

Protective role of Toll-like receptor 4 in experimental gonococcal infection of female mice.

Mucosal immunology

Packiam M,Wu H,Veit SJ,Mavrogiorgos N,Jerse AE,Ingalls RR

12-9021 was used in Flow cytometry/Cell sorting to provide evidence that TLR4-regulated factors modulate early inflammatory responses to gonococcal infection in the female reproductive tract and control bacterial replication.

Sun Jan 01 00:00:00 UTC 2012

Macrophage p38 mitogen-activated protein kinase activity regulates invariant natural killer T-cell responses during Borrelia burgdorferi infection.

The Journal of infectious diseases

Hawley K,Navasa N,Olson CM,Bates TC,Garg R,Hedrick MN,Conze D,Rincón M,Anguita J

12-9021 was used in Flow cytometry/Cell sorting to demonstrate that p38 mitogen-activated protein kinase-mediated responses are critical components to the immune response to Borrelia burgdorferi.

Sun Jul 15 00:00:00 UTC 2012

Comparison of blood monocytes and adipose tissue macrophages in a mouse model diet-induced weight gain.

Comparative medicine

McFarlin BK,Carpenter KC,Strohacker K,Breslin WL

12-9021 was used in Flow cytometry/Cell sorting to compare blood monocytes and adipose tissue macrophages in diet-induced weight gain in mice.

Sat Dec 01 00:00:00 UTC 2012

Impact of enzymatic tissue disintegration on the level of surface molecule expression and immune cell function.

European journal of microbiology & immunology

Autengruber A,Gereke M,Hansen G,Hennig C,Bruder D

12-9021 was used in Flow cytometry/Cell sorting to indicate that enzymatic tissue disintegration can have profound effects on the expression of a variety of cell-surface molecules.

Fri Jun 01 00:00:00 UTC 2012

Characterization of iNOS(+) Neutrophil-like ring cell in tumor-bearing mice.

Journal of translational medicine

Virtuoso LP,Harden JL,Sotomayor P,Sigurdson WJ,Yoshimura F,Egilmez NK,Minev B,Kilinc MO

12-9021 was used in Flow cytometry/Cell sorting to investigate whether inducible nitric oxide synthase can serve as a specific marker to identify a homogenous myeloid-derived suppressor cell subset.

Mon Jul 30 00:00:00 UTC 2012

Sex-specific signaling through Toll-Like Receptors 2 and 4 contributes to survival outcome of Coxsackievirus B3 infection in C57Bl/6 mice.

Biology of sex differences

Roberts BJ,Dragon JA,Moussawi M,Huber SA

12-9021 was used in Flow cytometry/Cell sorting to determine which TLRs show differential expression in CVB3 infected mice corresponding to male susceptibility and female resistance in Coxsackievirus B3.

Sat Dec 15 00:00:00 UTC 2012

The sensing of environmental stimuli by follicular dendritic cells promotes immunoglobulin A generation in the gut.

Immunity

Suzuki K,Maruya M,Kawamoto S,Sitnik K,Kitamura H,Agace WW,Fagarasan S

12-9021 was used in Flow cytometry/Cell sorting to investigate whether follicular dendritic cells within the germinal centers directly contribute to the IgA production in Peyer's patches, showing that sensing of environmental stimuli promotes IgA production.

Fri Jul 23 00:00:00 UTC 2010

The Toll-like receptor 2 (TLR2) ligand FSL-1 is internalized via the clathrin-dependent endocytic pathway triggered by CD14 and CD36 but not by TLR2.

Immunology

Shamsul HM,Hasebe A,Iyori M,Ohtani M,Kiura K,Zhang D,Totsuka Y,Shibata K

12-9021 was used in Flow cytometry/Cell sorting to investigate how FSL-1 is processed in macrophages after recognition by Toll-like receptor 2.

Tue Jun 01 00:00:00 UTC 2010

Theiler's virus infection induces TLR3-dependent upregulation of TLR2 critical for proinflammatory cytokine production.

Glia

So EY,Kim BS

12-9021 was used in Flow cytometry/Cell sorting to indicate that TMEV utilizes TLR3-induced TLR2 to induce inflammatory cytokines, which are critical to the development of immune-mediated demyelinating disease.

Sat Aug 15 00:00:00 UTC 2009

Requirement for lymphoid tissue-inducer cells in isolated follicle formation and T cell-independent immunoglobulin A generation in the gut.

Immunity

Tsuji M,Suzuki K,Kitamura H,Maruya M,Kinoshita K,Ivanov II,Itoh K,Littman DR,Fagarasan S

12-9021 was used in Flow cytometry/Cell sorting to investigate the sites and mechanisms for T cell-independent IgA synthesis.

Fri Aug 15 00:00:00 UTC 2008

Toll-like receptor 2 down-regulation in established mouse allergic lungs contributes to decreased mycoplasma clearance.

American journal of respiratory and critical care medicine

Wu Q,Martin RJ,Lafasto S,Efaw BJ,Rino JG,Harbeck RJ,Chu HW

12-9021 was used in Flow cytometry/Cell sorting to investigate the effect of established allergic airway inflammation on host immunity and the elimination of Mycoplasma pneumoniae from the lungs.

Tue Apr 01 00:00:00 UTC 2008

Toll-like receptor 2 deficiency delays pneumococcal phagocytosis and impairs oxidative killing by granulocytes.

Infection and immunity

Letiembre M,Echchannaoui H,Bachmann P,Ferracin F,Nieto C,Espinosa M,Landmann R

12-9021 was used in Flow cytometry/Cell sorting to investigate phagocytosis and bactericidal activity of wild-type (WT) and Toll-like receptor 2 (TLR2)-deficient granulocytes against live S. pneumoniae.

Thu Dec 01 00:00:00 UTC 2005

Bioinformatics

Protein Aliases: CD282; TLR-2; Toll-like receptor 2

View more View less

Gene Aliases: Ly105; Tlr2

View more View less

UniProt ID: (Mouse) Q9QUN7

View more View less

Entrez Gene ID: (Mouse) 24088

View more View less

Function(s)

receptor activity transmembrane signaling receptor activity protein binding signaling pattern recognition receptor activity triacyl lipopeptide binding diacyl lipopeptide binding peptidoglycan binding protein heterodimerization activity lipoteichoic acid binding lipopeptide binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD282 (TLR2) Monoclonal Antibody (6C2), PE, eBioscience™

CD282 (TLR2) Antibody (12-9021-82) in Flow

Product Details

12-9021-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!