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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • TLR2 Antibodies

          Invitrogen

          CD282 (TLR2) Monoclonal Antibody (TL2.1), Functional Grade, eBioscience™

          10 Published Figures
          58 References
          View all (75) TLR2 antibodies

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          Datasheet
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          Datasheet
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          Cite CD282 (TLR2) Monoclonal Antibody (TL2.1), Functional Grade, eBioscience™

          • Antibody Testing Data (1)
          • Published Figures (10)
          CD282 (TLR2) Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          CD282 (TLR2) Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 11

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          CD282 (TLR2) Antibody (16-9922-82) in Flow

          Staining of normal human peripheral blood cells with Anti-Human CD282 (TLR2) PE. Appropriate isotype controls were used (open histogram). Cells in the monocyte population were used for analysis. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          CD282 (TLR2) Antibody in Flow Cytometry (Flow)
          CD282 (TLR2) Antibody in Immunocytochemistry (ICC/IF)
          CD282 (TLR2) Antibody in Western Blot (WB)
          CD282 (TLR2) Antibody in Flow Cytometry (Flow)
          CD282 (TLR2) Antibody in Flow Cytometry (Flow)
          CD282 (TLR2) Antibody in Flow Cytometry (Flow)
          CD282 (TLR2) Antibody in Flow Cytometry (Flow)
          CD282 (TLR2) Antibody in Flow Cytometry (Flow)
          CD282 (TLR2) Antibody in Flow Cytometry (Flow)
          CD282 (TLR2) Antibody in Flow Cytometry (Flow)
          CD282 (TLR2) Antibody in Neutralization (Neu)

          Product Details

          16-9922-82

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          -
          View 4 publications 4 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          -
          View 2 publications 2 publications

          Immunohistochemistry (Frozen) (IHC (F))

          -
          View 2 publications 2 publications

          Immunocytochemistry (ICC/IF)

          -
          View 2 publications 2 publications

          Flow Cytometry (Flow)

          1 µg/test
          View 16 publications 16 publications

          ELISA (ELISA)

          -
          View 1 publication 1 publication

          Neutralization (Neu)

          Assay-Dependent
          View 15 publications 15 publications

          Functional Assay (Functional)

          Assay-Dependent
          View 10 publications 10 publications

          Inhibition Assays (IA)

          -
          View 2 publications 2 publications

          In vitro Assay (IV)

          -
          View 2 publications 2 publications

          Miscellaneous PubMed (MISC)

          -
          View 2 publications 2 publications
          Product Specifications

          Species Reactivity

          Human

          Published species

          Human, Mouse

          Host/Isotype

          Mouse / IgG2a, kappa

          Recommended Isotype Control

          Mouse IgG2a kappa Isotype Control (eBM2a), Functional Grade, eBioscience™

          Class

          Monoclonal

          Type

          Antibody

          Clone

          TL2.1

          Conjugate

          Functional Grade Functional Grade Functional Grade
          • Unconjugated
          • Alexa Fluor 488 
          • APC 
          • Biotin 
          • FITC
          • PE 
          • Request custom conjugation

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2

          Contains

          no preservative

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_469283

          Product Specific Information

          Description: The TL2.1 monoclonal antibody reacts with human Toll-like receptor 2 (TLR2). To date, at least ten members of the Toll family have been identified in human. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation. TLR2 is expressed by peripheral blood monocytes and is responsible for distinguishing different pathogens. TL2.1, a blocking antibody, has been used to study the role of TLR-2 as a pattern recognition receptor in microbial lipoprotein/lipopeptide induced cytokine production from human peripheral blood mononuclear cells. TL2.1 has been reported to immunoprecipitate human TLR2 (~90 kDa) from PBMC and HMEC.

          Applications Reported: TL2.1 has been reported for use in flow cytometric analysis and blocking of TLR2-mediated cytokine production. Inhibition of cytokine production has been reported in the literature using Listeria monocytogenes and peptidoglycans. Optimal conditions for the inhibition assays using TL2.1 and specific stimulatory reagents/suppliers have to be evaluated by individual investigators. Neutralization Protocol: (Important note: make sure to eliminate LPS contamination in the culture system) 1) Prepare target cells of interest at the concentration of 2 x 10^6 /mL in 10% FBS-IMDM (or 5% human serum). 2) Pre-incubate the cells with 10-20 µg/mL of TL2.1 or isotype control antibody at room temperature for 30min. 3) Add the stimuli and incubate at 37°C, 5% CO2 for 14 hours. Harvest the supernatants. 4) Follow the protocol of eBioscience Ready-SET-Go ELISA set to detect IL-6 or TNF alpha production.

          Applications Tested: The TL2.1 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Storage and handling: Use in a sterile environment.

          Filtration: 0.2 µm post-manufacturing filtered.

          Purity: Greater than 90%, as determined by SDS-PAGE.

          Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

          Aggregation: Less than 10%, as determined by HPLC.

          Target Information

          TLR2 is a member of the Toll-like receptor (TLR) family which play a fundamental role in pathogen recognition and activation of innate immunity. TLR proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors such as Protein Kinase C (PKC) alpha/beta and NF-kappa-B. TLR2 can form heterodimers with either TLR1 or TLR6, and as a heterodimer, can recognize a variety of bacterial and mycoplasma lipoproteins respectively. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. The various TLRs exhibit different patterns of expression. TLR2 is expressed most abundantly in peripheral blood leukocytes, and mediates host response to Gram-positive bacteria and yeast via stimulation of NF-kappaB. TLR2 aids in the recognition of pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. Ten human homologs of TLRs (TLR1-10) have been described. TLR2 is expressed in human cells such as tonsils, microglia, lymph nodes, and appendices, activated B-cells in germinal centers. CD14+ monocytes express the highest level of TLR2 followed by CD15+ granulocytes, CD19+ B-cells, and CD3+ T-cells. The expression of TLR2 on different cell types are regulated by different immune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: CD282; TLR-2; Toll-like receptor 2; Toll/interleukin-1 receptor-like protein 4

          View more View less

          Gene Aliases: CD282; TIL4; TLR2

          View more View less

          UniProt ID: (Human) O60603

          View more View less

          Entrez Gene ID: (Human) 7097

          View more View less

          Function(s)
          lipopolysaccharide binding lipopolysaccharide receptor activity receptor activity transmembrane signaling receptor activity protein binding signaling pattern recognition receptor activity Toll-like receptor binding triacyl lipopeptide binding peptidoglycan binding protein heterodimerization activity
          Process(es)
          cytokine secretion involved in immune response MyD88-dependent toll-like receptor signaling pathway apoptotic process inflammatory response immune response signal transduction I-kappaB phosphorylation positive regulation of gene expression positive regulation of Wnt signaling pathway lipopolysaccharide-mediated signaling pathway interleukin-10 production positive regulation of chemokine production positive regulation of interferon-beta production positive regulation of interleukin-12 production positive regulation of interleukin-18 production positive regulation of interleukin-6 production positive regulation of interleukin-8 production positive regulation of tumor necrosis factor production positive regulation of toll-like receptor signaling pathway toll-like receptor 2 signaling pathway toll-like receptor TLR1:TLR2 signaling pathway toll-like receptor TLR6:TLR2 signaling pathway positive regulation of NF-kappaB import into nucleus detection of triacyl bacterial lipopeptide detection of diacyl bacterial lipopeptide innate immune response positive regulation of transcription from RNA polymerase II promoter regulation of cytokine secretion positive regulation of inflammatory response defense response to Gram-positive bacterium positive regulation of NF-kappaB transcription factor activity positive regulation of nitric-oxide synthase biosynthetic process cellular response to bacterial lipopeptide cellular response to lipoteichoic acid cellular response to diacyl bacterial lipopeptide cellular response to triacyl bacterial lipopeptide
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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