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Invitrogen
CD289 (TLR9) Monoclonal Antibody (M9.D6), eBioscience™
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FIGURE: 1 / 2
CD289 (TLR9) Antibody (14-9093-82) in Flow
Frontiers in immunology 2015 -
Figure 4 CpG immunization induces APC maturation in the lung . BALB/cByJ mice were immunized intranasally. (A) Representative FACS plots from the lung at day 1 show (i) interstitial macrophages (F4/80 + ), (ii) alveolar macrophages (F4/80 + CD11c + ), and (iii) conventional DCs (CD11c + ). DCs, interstitial macrophages, and alveolar macrophage populations were enumerated at day 1 (B) and day 3 (F) in the lu... View More
Product Details
14-9093-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
M9.D6
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_468573
Product Specific Information
Description: M9.D6 is generated against a peptide derived from the extracellular portion of mouse TLR9. Predominantly expressed as an intracellular protein, TLR9 is a ~115-120 kDa molecule which mediates response to unmethylated CpG dinucleotides in bacterial DNA. CpG DNA induces a strong T-helper-1-like inflammatory response and the proliferation of TLR9+ B cells. When stimulated with CpG DNA, TLR9-deficient (TLR9-/-) mice lacked splenocyte proliferation, inflammatory cytokine production from macrophages, and dendritic cell maturation, as compared with normal mice. To date, at least ten members of the Toll family have been identified. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Members of the TLR family are involved in recognition and response to different microbial components including lipoproteins, peptidoglycans, and nucleic acids and play important roles in innate immunity and inflammation. TLR9 is not detected by flow cytometry using this antibody on RBC-lysed mouse splenocytes stained for intracellular TLR9. This may be due to limitations of antigen detection by flow cytometry. Further studies are needed to determine the relationship between mRNA expression and protein detection.
Applications Reported: This M9.D6 antibody has been reported for use in intracellular flow cytometric analysis, and immunoblotting (WB). (Fluorochrome conjugated M9.D6 is recommended for use in intracellular flow cytometry.).
Applications Tested: The M9.D6 antibody has been tested by intracellular flow cytometric analysis of fixed and permeabilized mouse TLR9 transfected cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The protein encoded by this gene is a member of the Toll-like receptor family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is preferentially expressed in immune cell rich tissues, such as spleen, lymph node, bone marrow and peripheral blood leukocytes. Studies in mice and human indicate that this receptor mediates cellular response to unmethylated CpG dinucleotides in bacterial DNA to mount an innate immune response.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD289; Toll-like receptor 9
Gene Aliases: Tlr9
UniProt ID: (Mouse) Q9EQU3
Entrez Gene ID: (Mouse) 81897
microglial cell activation
regulation of protein phosphorylation
toll-like receptor signaling pathway
response to molecule of bacterial origin
immune system process
regulation of dendritic cell cytokine production
MyD88-dependent toll-like receptor signaling pathway
inflammatory response
immune response
signal transduction
I-kappaB phosphorylation
response to virus
positive regulation of autophagy
positive regulation of gene expression
maintenance of gastrointestinal epithelium
negative regulation of NF-kappaB transcription factor activity
tumor necrosis factor production
negative regulation of interleukin-6 production
negative regulation of interleukin-8 production
positive regulation of chemokine production
positive regulation of granulocyte macrophage colony-stimulating factor production
positive regulation of interferon-beta production
positive regulation of interleukin-10 production
positive regulation of interleukin-12 production
positive regulation of interleukin-18 production
positive regulation of interleukin-6 production
positive regulation of interleukin-8 production
positive regulation of tumor necrosis factor production
negative regulation of toll-like receptor signaling pathway
positive regulation of toll-like receptor signaling pathway
toll-like receptor 9 signaling pathway
positive regulation of NF-kappaB import into nucleus
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of JUN kinase activity
positive regulation of interferon-gamma biosynthetic process
innate immune response
positive regulation of interferon-alpha biosynthetic process
positive regulation of interferon-beta biosynthetic process
positive regulation of transcription from RNA polymerase II promoter
regulation of cytokine secretion
regulation of inflammatory response
positive regulation of inflammatory response
defense response to Gram-negative bacterium
regulation of B cell activation
positive regulation of NF-kappaB transcription factor activity
defense response to virus
positive regulation of nitric-oxide synthase biosynthetic process
negative regulation of calcium-transporting ATPase activity
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