Invitrogen

CD289 (TLR9) Monoclonal Antibody (eB72-1665), eBioscience™

5 Published Figures

15 References

View all (39) TLR9 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (5)

CD289 (TLR9) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 6

CD289 (TLR9) Antibody (14-9099-82) in Flow

HEK 293 parent cells (left panel) and 293/hTLR9 transfected cells (right panel) were fixed and permeabilized and subsequently stained with either Anti-Rat IgG FITC (Product # 11-4811-85) alone (thin line) or with 1 µg of Anti-Human CD289 (TLR9) Purified followed by Anti-Rat IgG FITC (thick line).

CD289 (TLR9) Antibody in Western Blot (WB)

CD289 (TLR9) Antibody in Western Blot, Immunoprecipitation (WB, IP)

CD289 (TLR9) Antibody in Western Blot, Flow Cytometry (WB, Flow)

Product Image Gallery

Product Details

14-9099-82

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

1 µg/test

View 8 publications 8 publications

Immunoprecipitation (IP)

Assay-Dependent

View 2 publications 2 publications

Inhibition Assays (IA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Hamster, Human

Host/Isotype

Rat / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

eB72-1665

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_468575

Product Specific Information

Description: eB72-1665 is generated against a portion of human toll-like receptor 9 (aa 273-288), a molecule reported to be expressed predominantly intracellularly. TLR9 is a ~115-120 kDa molecule, which mediates response to unmethylated CpG dinucleotides in bacterial DNA. CpG DNA induces a strong T-helper-1-like inflammatory response and the proliferation of TLR9-positive human B cells. When stimulated with CpG DNA, TLR9-deficient (TLR9-/-) mice lacked splenocyte proliferation, inflammatory cytokine production from macrophages, and dendritic cell maturation, as compared with normal mice. To date, at least twelve members of the Toll family have been identified. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Members of the TLR family are involved in recognition and response to different microbial components including lipoproteins, peptidoglycans, and nucleic acids and play important roles in innate immunity and inflammation. TLR9 is not detected by flow cytometry using this antibody on lysed whole human blood and/or isolated human PBMC stained for cell surface or intracellular TLR9. This may be due to limitations of antigen detection by flow cytometry. Human pDCs matured in the presence of IL-3 have been reported to stain with eB72-1665 by immunofluorescence microscopy (Nat Immunol. 5:190). Human Epithelial Cell lines were also reported to stain with this mAb (J. Immunol. 173: 1219). Further studies are needed to determine the relationship between mRNA expression and protein detection by flow cytometry.

Applications Reported: This eB72-1665 antibody has been reported for use in intracellular flow cytometric analysis, immunoprecipitation, and immunoblotting (WB). (Fluorochrome conjugated eB72-1665 is recommended for use in intracellular flow cytometry.) Preliminary in vitro blocking assays suggested that eB72-1665 may not be effective in blocking of CpG binding. Please contact us for further information.

Applications Tested: The eB72-1665 antibody has been tested by intracellular flow cytometric analysis of hTLR9 transfected cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The protein encoded by this gene is a member of the Toll-like receptor family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is preferentially expressed in immune cell rich tissues, such as spleen, lymph node, bone marrow and peripheral blood leukocytes. Studies in mice and human indicate that this receptor mediates cellular response to unmethylated CpG dinucleotides in bacterial DNA to mount an innate immune response.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

TLR9 expression in chronic lymphocytic leukemia identifies a promigratory subpopulation and novel therapeutic target.

Blood

Kennedy E,Coulter E,Halliwell E,Profitos-Peleja N,Walsby E,Clark B,Phillips EH,Burley TA,Mitchell S,Devereux S,Fegan CD,Jones CI,Johnston R,Chevassut T,Schulz R,Seiffert M,Agathanggelou A,Oldreive C,Davies N,Stankovic T,Liloglou T,Pepper C,Pepper AGS

14-9099-82 was used in Inhibition Assays to explore the molecular signalling mechanisms of chronic lymphocytic leukaemia to help improve current treatment options.

Thu Jun 03 00:00:00 UTC 2021

Human CD40 ligand-expressing type 3 innate lymphoid cells induce IL-10-producing immature transitional regulatory B cells.

The Journal of allergy and clinical immunology

Komlósi ZI,Kovács N,van de Veen W,Kirsch AI,Fahrner HB,Wawrzyniak M,Rebane A,Stanic B,Palomares O,Rückert B,Menz G,Akdis M,Losonczy G,Akdis CA

14-9099 was used in Flow cytometry/Cell sorting to investigate the ILC3-B-cell interaction that probably takes place in human tonsils.

Sun Jul 01 00:00:00 UTC 2018

Altered Toll-like receptor expression and function in HPV-associated oropharyngeal carcinoma.

Oncotarget

Tobouti PL,Bolt R,Radhakrishnan R,de Sousa SCOM,Hunter KD

Published figure using CD289 (TLR9) monoclonal antibody (Product # 14-9099-82) in Flow Cytometry

Tue Jan 02 00:00:00 UTC 2018

ICOS⁺ Foxp3⁺ TILs in gastric cancer are prognostic markers and effector regulatory T cells associated with Helicobacter pylori.

International journal of cancer

Nagase H,Takeoka T,Urakawa S,Morimoto-Okazawa A,Kawashima A,Iwahori K,Takiguchi S,Nishikawa H,Sato E,Sakaguchi S,Mori M,Doki Y,Wada H

14909982 was used in flow cytometry to establish the T Cell receptor-inducible costimulator as a promising target for direct T regulatory cell-targeting therapeutic agents for gastric cancer

Wed Feb 01 00:00:00 UTC 2017

Toll-like receptor-9 in hypoxic nasopharyngeal carcinoma cells and its correlation with cell proliferation and apoptosis.

Oncology letters

Zhang F,Wang D,Chen F

Published figure using CD289 (TLR9) monoclonal antibody (Product # 14-9099-82) in Western Blot

Fri Dec 01 00:00:00 UTC 2017

Lactoferrin suppresses the Epstein-Barr virus-induced inflammatory response by interfering with pattern recognition of TLR2 and TLR9.

Laboratory investigation; a journal of technical methods and pathology

Zheng Y,Qin Z,Ye Q,Chen P,Wang Z,Yan Q,Luo Z,Liu X,Zhou Y,Xiong W,Ma J,Li G

14-9099 was used in Immunoprecipitation to report that lactoferrin reduces synthesis of IL-8 and MCP-1 induced by Epstein-Barr virus, by suppressing NF-κB activity.

Sat Nov 01 00:00:00 UTC 2014

A consensus surface activation marker signature is partially dependent on human immunodeficiency virus type 1 Nef expression within productively infected macrophages.

Retrovirology

Babu R,Brown A

14-9099 was used in Flow cytometry/Cell sorting to determine whether macrophage surface marker phenotype in non-polarised cultures is altered by HIV infection independently of host genetic background.

Mon Dec 16 00:00:00 UTC 2013

Double-stranded RNA-mediated TLR3 activation is enhanced by CD14.

Immunity

Lee HK,Dunzendorfer S,Soldau K,Tobias PS

14-9099 was used in Immunocytochemistry-immunoflourescence to study the effect of CD14 on double-stranded RNA-mediated Toll-like receptor 3 activation.

Wed Feb 01 00:00:00 UTC 2006

Human monocyte-derived dendritic cells express TLR9 and react directly to the CpG-A oligonucleotide D19.

Journal of leukocyte biology

Hoene V,Peiser M,Wanner R

14-9099 was used in Flow cytometry/Cell sorting to indicate that moDC themselves sense viral and bacterial DNA and do not need help from pDC.

Fri Dec 01 00:00:00 UTC 2006

Activation of TLR-9 induces IL-8 secretion through peroxynitrite signaling in human neutrophils.

Journal of immunology (Baltimore, Md. : 1950)

József L,Khreiss T,El Kebir D,Filep JG

Sun Jan 15 00:00:00 UTC 2006

Cytoplasmic targeting motifs control localization of toll-like receptor 9.

The Journal of biological chemistry

Leifer CA,Brooks JC,Hoelzer K,Lopez J,Kennedy MN,Mazzoni A,Segal DM

14-9099 was used in Flow cytometry/Cell sorting to investigate the structural features of TLR9 that allow its correct localisation within the cell.

Fri Nov 17 00:00:00 UTC 2006

Human lung cancer cells express functionally active Toll-like receptor 9.

Respiratory research

Droemann D,Albrecht D,Gerdes J,Ulmer AJ,Branscheid D,Vollmer E,Dalhoff K,Zabel P,Goldmann T

14-9099 was used in Flow cytometry/Cell sorting to investigate the expression of Toll-like receptor 9 in human lung cancer cells.

Tue Jan 04 00:00:00 UTC 2005

TLR9 is localized in the endoplasmic reticulum prior to stimulation.

Journal of immunology (Baltimore, Md. : 1950)

Leifer CA,Kennedy MN,Mazzoni A,Lee C,Kruhlak MJ,Segal DM

Published figure using CD289 (TLR9) monoclonal antibody (Product # 14-9099-82) in Flow Cytometry

Thu Jul 15 00:00:00 UTC 2004

TLR9 is localized in the endoplasmic reticulum prior to stimulation.

Journal of immunology (Baltimore, Md. : 1950)

Leifer CA,Kennedy MN,Mazzoni A,Lee C,Kruhlak MJ,Segal DM

Published figure using CD289 (TLR9) monoclonal antibody (Product # 14-9099-82) in Flow Cytometry

Thu Jul 15 00:00:00 UTC 2004

TLR9 is localized in the endoplasmic reticulum prior to stimulation.

Journal of immunology (Baltimore, Md. : 1950)

Leifer CA,Kennedy MN,Mazzoni A,Lee C,Kruhlak MJ,Segal DM

Published figure using CD289 (TLR9) monoclonal antibody (Product # 14-9099-82) in Flow Cytometry

Thu Jul 15 00:00:00 UTC 2004

Bioinformatics

Protein Aliases: CD289; Toll-like receptor 9

View more View less

Gene Aliases: CD289; TLR9; UNQ5798/PRO19605

View more View less

UniProt ID: (Human) Q9NR96

View more View less

Entrez Gene ID: (Human) 54106

View more View less

Function(s)

transmembrane signaling receptor activity interleukin-1 receptor binding 1-phosphatidylinositol-3-kinase activity siRNA binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD289 (TLR9) Monoclonal Antibody (eB72-1665), eBioscience™

CD289 (TLR9) Antibody (14-9099-82) in Flow

Product Details

14-9099-82

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Inhibition Assays (IA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!