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Invitrogen
CD29 (Integrin beta 1) Monoclonal Antibody (KMI6), eBioscience™
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-WB-20170216130641.jpg?time=20250104 "CD29 (Integrin beta 1) Antibody in Western Blot (WB)")
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-WB-20170216130641.jpg?time=20250104?time=20250104 "CD29 (Integrin beta 1) Antibody (14-0292-82) in WB")
Product Details
14-0292-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
KMI6
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_914295
Product Specific Information
Description: The KMI6 monoclonal antibody reacts with mouse CD29 (integrin beta1), a 120-130 kDa member of the beta integrin family expressed by leukocytes, endothelial, smooth muscle and epithelial cells. CD29 binds non-covalently with the alpha integrins CD49a-f, to form the VLA-1 through VLA-6 complexes, respectively, and also with CD51. These alpha beta integrin heterodimers are capable of mediating a variety of cellular responses including adhesion, trafficking, proliferaton and differentiation. All of the integrins which include CD29 bind to extracellular matrix proteins including collagen, laminin, fibronectin and vitronectin, whereas some CD29-containing integrins also interact with cellular receptors including VCAM-1 and MadCAM-1. The KMI6 antibody has been shown to be an activating antibody for beta1 integrins, enhancing lymphocyte binding to fibronectin.
Applications Reported: This KMI6 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistology staining of frozen tissue sections. The KMI6 antibody has also been found useful for immunoblotting, recognizing a protein of approximately 120 kD under non-reducing conditions.
Applications Tested: This KMI6 antibody has been tested by flow cytometric analysis of mouse splenocytes and thymocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The KMI6 antibody has also been tested by immunoblotting at 2 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
ITGB1 (Integrin Subunit Beta 1, beta1 integrin subunit, GPIIa, CD29) is a 110 kDa cell surface glycoprotein that is widely expressed by a variety of cells including all leucocytes. ITGB1 forms non-covalently linked heterodimers with at least 6 different alpha chains (alpha1-alpha6, CDa-f) determining the binding properties of beta1 (VLA) integrins. ITGB1 is a cell adhesion molecule appearing on platelets, as the common Beta subunit of the very late activation antigen (VLA), and as a component of various protein complexes binding to extracellular matrix proteins. Decreased expression of ITGB1 correlates with acquiring multidrug resistance of tumor cells in the presence of anti-tumor drug. ITGB1 is up-regulated in leukocytes during emigration and extravascular migration and appear to be critically involved in regulating the immune cell trafficking from blood to tissue. Further, ITGB1 also regulates tissue damage and disease symptoms related to inflammatory bowel disease. Through an ITGB1-dependent mechanism, fibronectin and type I collagen enhance cytokine secretion of human airway smooth muscle in response to IL-1beta. More than 8 beta subunits with numerous splice variant isoforms have been identified in mammals. There are two major forms of integrin beta1: beta1A and beta1D, which differ in 13 amino acids. The distribution pattern in adult tissues for integrin beta types are mutually exclusive. Beta1A is present in all tissues, except cardiac and skeletal muscle which express the beta1D variant.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD29; Fibronectin receptor subunit beta; Integrin beta-1; Integrin beta1; ITBG1D; VLA-4 subunit beta
Gene Aliases: 4633401G24Rik; AA409975; AA960159; CD29; ENSMUSG00000051907; Fnrb; Gm9863; gpIIa; Itgb1
UniProt ID: (Mouse) Q3TWH6
Entrez Gene ID: (Mouse) 16412
glycoprotein binding
fibronectin binding
protease binding
actin binding
receptor activity
receptor binding
integrin binding
protein binding
collagen binding
kinase binding
protein kinase binding
protein domain specific binding
protein complex binding
peptide binding
laminin binding
metal ion binding
protein heterodimerization activity
cell adhesion molecule binding
alpha-actinin binding
collagen binding involved in cell-matrix adhesion
G1/S transition of mitotic cell cycle
in utero embryonic development
cell fate specification
tissue homeostasis
cell migration involved in sprouting angiogenesis
cellular calcium ion homeostasis
cell adhesion
leukocyte cell-cell adhesion
cell-matrix adhesion
calcium-independent cell-matrix adhesion
transforming growth factor beta receptor signaling pathway
integrin-mediated signaling pathway
regulation of G-protein coupled receptor protein signaling pathway
positive regulation of cell proliferation
negative regulation of cell proliferation
germ cell migration
visual learning
regulation of collagen catabolic process
positive regulation of cell-substrate adhesion
positive regulation of neuron projection development
cell migration
formation of radial glial scaffolds
positive regulation of cell migration
neuron projection development
negative regulation of cell projection organization
cell-substrate adhesion
protein transport within lipid bilayer
heterotypic cell-cell adhesion
negative regulation of Rho protein signal transduction
positive regulation of apoptotic process
stress fiber assembly
positive regulation of MAPK cascade
sarcomere organization
negative regulation of cell differentiation
negative regulation of neuron differentiation
positive regulation of neuron differentiation
positive regulation of endocytosis
axon extension
cardiac muscle tissue development
dendrite morphogenesis
positive regulation of peptidyl-tyrosine phosphorylation
leukocyte tethering or rolling
regulation of cell cycle
cardiac muscle cell differentiation
bicellular tight junction assembly
positive regulation of establishment of protein localization to plasma membrane
negative regulation of anoikis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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