Invitrogen

CD29 (Integrin beta 1) Monoclonal Antibody (TS2/16), PE-Cyanine7, eBioscience™

CD29 (Integrin beta 1) Antibody in Flow Cytometry (Flow)

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CD29 (Integrin beta 1) Antibody (25-0299-42) in Flow

Normal human peripheral blood cells were stained with Mouse IgG1 kappa Isotype Control, PE-Cyanine7 (Product # 25-4714-42) (blue histogram) or CD29 Monoclonal Antibody, PE-Cyanine7 (purple histogram). Cells in the monocyte gate were used for analysis.

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Product Details

25-0299-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.5 µg)/test

View 14 publications 14 publications

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

TS2/16

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2848300

Product Specific Information

Description: The TS2/16 monoclonal antibody reacts with human CD29, also known as integrin beta 1, an approximately 130 kDa single-pass transmembrane glycoprotein. CD29 complexes with one of nine integrin alpha subunits to form the very late antigen (VLA) subfamily of adhesion molecules. Integrin heterodimers containing CD29 are involved in cell-cell and cell-matrix adhesion. CD29 is expressed broadly on lymphocytes and monocytes, with lower levels of expression on granulocytes. The TS2/16 antibody has been found to possess activating activity for beta 1 integrins.

Applications Reported: This IP26 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This IP26 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor 450 is an alternative to Pacific Blue. eFluor 450 emits at 446 nm and is excited with the violet laser line (405 nm). Please make sure that your instrument is capable of detecting this fluorochrome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser

Target Information

CD29, also known as ITGB1 or Integrin Subunit Beta 1, is a 110 kDa cell surface glycoprotein widely expressed by various cells, including all leukocytes. It forms non-covalently linked heterodimers with at least six different alpha integrins (CD49a-f), creating VLA-1 through VLA-6 complexes, as well as with CD51. These alpha-beta integrin heterodimers mediate a range of cellular responses, including adhesion, trafficking, proliferation, and differentiation. CD29-containing integrins bind to extracellular matrix proteins such as collagen, laminin, fibronectin, and vitronectin. Some integrins also interact with cellular receptors like VCAM-1 and MadCAM-1. CD29 plays a crucial role in immune cell trafficking from blood to tissue, particularly during leukocyte emigration and extravascular migration. It is involved in regulating tissue damage and disease symptoms related to inflammatory bowel disease. In the presence of anti-tumor drugs, decreased expression of ITGB1 correlates with multidrug resistance in tumor cells. ITGB1-dependent mechanisms enhance cytokine secretion in human airway smooth muscle in response to IL-1beta, facilitated by fibronectin and type I collagen. More than eight beta subunits with numerous splice variant isoforms have been identified in mammals, including two major forms: beta1A and beta1D. Beta1A is present in all tissues except cardiac and skeletal muscle, which express the beta1D variant.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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