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CD298 Antibody (PA5-106750) in ICC/IF
Immunocytochemistry analysis of CD298 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with CD298 polyclonal antibody (Product # PA5-106750) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-106750
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ATP1B3(Accession P54709), corresponding to amino acid residues K86-Q136.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2854418
Product Specific Information
Antibody detects endogenous levels of total ATP1B3.
Target Information
The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 3 subunit. This gene encodes a beta 3 subunit. A pseudogene exists for this gene, and it is located on chromosome 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATPase, Na+K+ transporting, beta polypeptide 3; ATPB-3; CD298; CD298 antigen; Na+/K+ -ATPase beta 3 subunit; Sodium Potassium ATPase; Sodium/potassium-dependent ATPase subunit beta-3; Sodium/potassium-transporting ATPase subunit beta-3
Gene Aliases: ATP1B3; ATPB-3; CD298; NKAB3S
UniProt ID: (Human) P54709, (Rat) Q63377
Entrez Gene ID: (Human) 483, (Rat) 25390
potassium ion transport
sodium ion transport
cellular sodium ion homeostasis
potassium ion import
metal ion transport
cellular potassium ion homeostasis
positive regulation of ATPase activity
sodium ion export from cell
protein stabilization
protein localization to plasma membrane
membrane repolarization
positive regulation of potassium ion transmembrane transporter activity
positive regulation of sodium ion export from cell
positive regulation of potassium ion import
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