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Invitrogen
CD298 Monoclonal Antibody (M016)
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Product Details
MA5-47672
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
M016
Immunogen
Clone (M016) was generated from a proprietary antigen related to the native human Na+/K+ ATPase beta3 subunit expressed in A431 epidermoid carcinoma cell line.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein G
Storage buffer
PBS with 1mg/mL BSA, 50% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2942653
Product Specific Information
Clone M016 mouse monoclonal antibody detects a 40 kDa* protein on SDS-PAGE "Native" immunoblots of human A431, LNCaP, MeWo, MDA-MB-231, and MCF7 cells. This antibody does not detect denatured Na+/K+ ATPase beta3 subunit. In addition, mass spectrometry analysis of immunoprecipitates using NM0161 in human A431 cell lysates confirms that this antibody only detects Na+/K+ ATPase beta3 protein.The antibody works for western blot, immunoprecipitation, ELISA, and immunocytochemistry, as well as detects the beta3 subunit on live cells.
Target Information
The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 3 subunit. This gene encodes a beta 3 subunit. A pseudogene exists for this gene, and it is located on chromosome 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATPB-3; CD298; CD298 antigen; Sodium Potassium ATPase; Sodium/potassium-dependent ATPase subunit beta-3; Sodium/potassium-transporting ATPase subunit beta-3
Gene Aliases: ATP1B3; ATPB-3; CD298
UniProt ID: (Human) P54709
Entrez Gene ID: (Human) 483
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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