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Invitrogen
CD2AP Monoclonal Antibody (5F8)
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FIGURE: 1 / 21
CD2AP Antibody (MA5-33009) in ICC/IF
Immunofluorescence analysis of CD2AP was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CD2AP Monoclonal Antibody (5F8) (Product # MA5-33009) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then with Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32723) at a... View More
Product Details
MA5-33009
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
5F8
Immunogen
E.coli-derived human CD2AP recombinant protein (Position: K253-K337).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2802643
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human K562 whole cell, human A431 whole cell, human HEK293 whole cell, human U20S whole cell, human HL-60 whole cell, human MCF-7 whole cell, human Hela whole cell, human PANC-1 whole cell, rat liver tissue, rat testicular tissue, mouse pancreas tissue, mouse testicular tissue, mouse RAW2467 whole cell. IHC: human mammary cancer, human mammary cancer, human colon cancer, human placenta tissue, mouse kidney tissue, rat brain tissue. ICC/IF: A431 cell. Flow: K562 cell, U20S cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
This gene encodes a scaffolding molecule that regulates the actin cytoskeleton. The protein directly interacts with filamentous actin and a variety of cell membrane proteins through multiple actin binding sites, SH3 domains, and a proline-rich region containing binding sites for SH3 domains. The cytoplasmic protein localizes to membrane ruffles, lipid rafts, and the leading edges of cells. It is implicated in dynamic actin remodeling and membrane trafficking that occurs during receptor endocytosis and cytokinesis. Haploinsufficiency of this gene is implicated in susceptibility to glomerular disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Adapter protein CMS; Cas ligand with multiple SH3 domains; Cas ligand with multiple Src homology 3 (SH3) domains; CD2-associated protein; mesenchyme to epithelium transition protein, SH3 domains; Mesenchyme-to-epithelium transition protein with SH3 domains 1; METS-1; SH3 domain-containing adapter protein; SH3 domain-containing adaptor protein METS-1
Gene Aliases: AL024079; C78928; CD2AP; CMS; METS-1; Mets1
UniProt ID: (Human) Q9Y5K6, (Mouse) Q9JLQ0
Entrez Gene ID: (Human) 23607, (Mouse) 12488, (Rat) 316258
cell cycle
mitotic nuclear division
vesicle organization
single organismal cell-cell adhesion
cell migration
negative regulation of transforming growth factor beta1 production
proteasome-mediated ubiquitin-dependent protein catabolic process
regulation of receptor-mediated endocytosis
cell division
positive regulation of protein localization to nucleus
regulation of actin cytoskeleton reorganization
protein complex assembly
substrate-dependent cell migration, cell extension
cytoskeleton organization
signal transduction
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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