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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • CD3 Antibodies

          Invitrogen

          CD3 Monoclonal Antibody (7D6)

          1 Published Figure
          42 References
          View all (501) CD3 antibodies

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          Datasheet
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          Cite CD3 Monoclonal Antibody (7D6)

          • Antibody Testing Data (2)
          • Published Figures (1)
          CD3 Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          CD3 Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

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          CD3 Antibody (MHCD0300) in Flow

          Flow cytometry analysis of CD3 was performed in Jurkat cells. Cells were fixed with 1% paraformaldehyde for 20 mins and blocked in PBS containing 2.5% BSA for 45 minutes at room temperature. Cells were labeled with CD3 Mouse Monoclonal Antibody (MHCD03004, red histogram) at a dilution of 1:20 or a matched isotype control (pink histogram) in PBS. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Rabbit Anti-Mouse Secondary Antibody (Product # A-11059) at a dilution of 1:400 for 30 minutes at room... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          CD3 Antibody in Flow Cytometry (Flow)
          CD3 Antibody in Flow Cytometry (Flow)
          CD3 Antibody in Immunohistochemistry (IHC)

          Product Details

          MHCD0300

          Applications
          Tested Dilution
          Publications

          Immunohistochemistry (IHC)

          -
          View 1 publication 1 publication

          Flow Cytometry (Flow)

          1:20
          View 7 publications 7 publications

          Miscellaneous PubMed (MISC)

          -
          View 34 publications 34 publications
          Product Specifications

          Species Reactivity

          Human

          Published species

          Human, Mouse, Non-human primate

          Host/Isotype

          Mouse / IgG2a

          Class

          Monoclonal

          Type

          Antibody

          Clone

          7D6

          Immunogen

          Human CD3 epsilon

          Conjugate

          Unconjugated Unconjugated Unconjugated
          • Alexa Fluor 488 
          • APC 
          • APC-Alexa Fluor 750
          • FITC
          • PE 
          • PE-Alexa Fluor 610
          • PE-Alexa Fluor 700
          • PE-Cyanine5.5
          • PE-Cyanine7
          • PE-Texas Red
          • TRI-COLOR
          • Request custom conjugation

          Form

          Liquid

          Purification

          purified

          Storage buffer

          PBS

          Contains

          0.1% sodium azide

          Storage conditions

          4°C, store in dark

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10372049

          Target Information

          The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

          Analyte Specific Reagent

          Bioinformatics

          Protein Aliases: CD247; CD247 antigen, zeta subunit; CD3 antigen, delta subunit; CD3 delta; CD3 TCR complex; CD3-epsilon; CD3d; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3e; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3g; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3Z antigen, zeta polypeptide (TiT3 complex); CD3zeta chain; FLJ18683; Leu-4; OKT3, delta chain; T cell antigen receptor complex epsilon subunit of T3; T-cell antigen receptor complex, epsilon subunit of T3; T-cell antigen receptor complex, gamma subunit of T3; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor T3 delta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T3/TCR complex; TCR zeta chain

          View more View less

          Gene Aliases: CD247; CD3-DELTA; CD3-GAMMA; CD3-ZETA; CD3D; CD3E; CD3G; CD3H; CD3Q; CD3Z; IMD17; IMD18; IMD19; IMD25; T3D; T3E; T3G; T3Z; TCRE; TCRZ

          View more View less

          UniProt ID: (Human) P20963, (Human) P04234, (Human) P07766, (Human) P09693

          View more View less

          Entrez Gene ID: (Human) 919, (Human) 915, (Human) 916, (Human) 917

          View more View less

          Function(s)
          transmembrane signaling receptor activity protein binding identical protein binding protein homodimerization activity transcription coactivator activity protein heterodimerization activity receptor signaling protein activity SH3 domain binding protein kinase binding receptor signaling complex scaffold activity T cell receptor binding
          Process(es)
          positive regulation of T cell anergy protein complex assembly cell surface receptor signaling pathway transmembrane receptor protein tyrosine kinase signaling pathway signal complex assembly G-protein coupled receptor signaling pathway response to nutrient positive regulation of gene expression negative regulation of gene expression T cell costimulation positive regulation of interferon-gamma production positive regulation of interleukin-4 production positive regulation of T cell proliferation T cell activation regulation of apoptotic process negative thymic T cell selection positive regulation of interleukin-2 biosynthetic process negative regulation of smoothened signaling pathway positive regulation of alpha-beta T cell proliferation positive regulation of peptidyl-tyrosine phosphorylation regulation of immune response positive regulation of calcium-mediated signaling T cell receptor signaling pathway apoptotic signaling pathway T cell differentiation positive thymic T cell selection positive regulation of transcription from RNA polymerase II promoter viral process Fc-gamma receptor signaling pathway involved in phagocytosis regulation of defense response to virus by virus establishment or maintenance of cell polarity protein transport regulation of lymphocyte apoptotic process
          It has to be done as per old AB suggested Products section.
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